##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511779_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1173396 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.878033502756104 31.0 29.0 33.0 24.0 34.0 2 29.087324313360536 31.0 29.0 33.0 25.0 34.0 3 29.103199601839446 31.0 28.0 34.0 24.0 34.0 4 32.67015568486683 35.0 32.0 37.0 26.0 37.0 5 32.1418557758847 35.0 32.0 37.0 24.0 37.0 6 32.10425551135337 35.0 32.0 37.0 23.0 37.0 7 31.840017351346006 35.0 32.0 37.0 19.0 37.0 8 31.692450800923133 35.0 32.0 37.0 19.0 37.0 9 32.86442854756621 37.0 32.0 39.0 18.0 39.0 10 32.52401917170333 37.0 32.0 39.0 17.0 39.0 11 32.56892813679269 37.0 32.0 39.0 17.0 39.0 12 32.26870894395413 37.0 32.0 39.0 16.0 39.0 13 32.268503557196375 37.0 32.0 39.0 15.0 39.0 14 32.83557213421556 38.0 32.0 40.0 11.0 41.0 15 32.744232978465924 38.0 32.0 40.0 10.0 41.0 16 32.572035357202516 37.0 32.0 40.0 10.0 41.0 17 32.477537847410424 37.0 31.0 40.0 10.0 41.0 18 32.3432916082891 37.0 31.0 40.0 10.0 41.0 19 32.35838625664311 37.0 31.0 40.0 8.0 41.0 20 32.15483093516596 37.0 31.0 40.0 2.0 41.0 21 31.97733160842546 37.0 31.0 40.0 2.0 41.0 22 32.033773764355765 37.0 31.0 40.0 2.0 41.0 23 31.843975946739207 37.0 31.0 40.0 2.0 41.0 24 31.580302813372466 37.0 31.0 40.0 2.0 41.0 25 30.995547965051866 37.0 30.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.022523788552998525 1101 2 -0.03153606550292665 1101 3 0.18473969442330684 1101 4 0.22503454928224897 1101 5 0.3966744548735335 1101 6 0.1378519028664229 1101 7 0.1885352496609336 1101 8 0.21934397721101107 1101 9 0.10008090791614421 1101 10 0.07895084831363164 1101 11 -0.16231531904918484 1101 12 -0.18355325040669612 1101 13 0.1835322980523557 1101 14 -0.04554042713697726 1101 15 -0.04994120120725398 1101 16 0.026954401811998707 1101 17 -0.11284534646055278 1101 18 0.02125622129798188 1101 19 -0.008782825268475847 1101 20 0.11883996259950891 1101 21 -0.13646869905037562 1101 22 -0.3216798851831939 1101 23 -0.21555210596151042 1101 24 -0.6076610086561125 1101 25 -0.35681299970146085 1102 1 0.6972902764199205 1102 2 0.517958326686859 1102 3 0.5659187170405531 1102 4 0.5223502908655107 1102 5 0.5883869106972597 1102 6 0.712135907913698 1102 7 0.7546318769313309 1102 8 0.6227173352110036 1102 9 0.8478457016261487 1102 10 1.0424711756830618 1102 11 0.9061717284425086 1102 12 0.861267757139089 1102 13 0.9928453852888666 1102 14 1.2427075531967233 1102 15 1.295708912658192 1102 16 1.2341882507655981 1102 17 1.3673984965107024 1102 18 1.2365178353217843 1102 19 1.5789105323293384 1102 20 1.5268540286263885 1102 21 1.408418290126125 1102 22 1.0502510263938802 1102 23 1.2140228717932544 1102 24 1.2564771581354002 1102 25 1.342174402205849 1103 1 0.5409066455476363 1103 2 0.4902440936595376 1103 3 0.6442382449653579 1103 4 0.7074848063803998 1103 5 0.6644437471156337 1103 6 0.6172723173113468 1103 7 0.723732930528211 1103 8 0.5099254031515521 1103 9 0.8620585441448156 1103 10 0.6993095876125324 1103 11 0.5536316827899626 1103 12 0.7121469174512853 1103 13 0.4609210010499609 1103 14 1.0138966125350635 1103 15 0.7793729278889927 1103 16 0.5173411668466557 1103 17 0.5505456811044667 1103 18 0.8089177336501834 1103 19 0.8909488984069114 1103 20 0.7468010995871808 1103 21 0.7982902536838345 1103 22 1.0397088276956765 1103 23 0.8207967421918667 1103 24 0.9272704956552111 1103 25 1.1495029808966777 1104 1 0.07738385497781763 1104 2 -0.06271085569073165 1104 3 0.05085634769231717 1104 4 0.0735927707218309 1104 5 0.15451755755229613 1104 6 0.1890970338006568 1104 7 0.14766550043834314 1104 8 0.3078195993508359 1104 9 0.30368295246771737 1104 10 0.26044493246714495 1104 11 0.4006058406975157 1104 12 0.3342958356135668 1104 13 0.41567865155838746 1104 14 0.29910004335797424 1104 15 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-0.5616499037859057 2314 5 -0.5873211338130275 2314 6 -0.6230862650448934 2314 7 -0.7765407356150362 2314 8 -0.5302556947545014 2314 9 -0.6295733828633772 2314 10 -0.6154343751263625 2314 11 -0.8367437712168524 2314 12 -0.8300826910998538 2314 13 -0.6242888052316076 2314 14 -0.7568761090614515 2314 15 -0.7768163109205837 2314 16 -0.621723745168353 2314 17 -0.671606131867307 2314 18 -0.6151996930378445 2314 19 -0.6858383673494828 2314 20 -0.39648305354895186 2314 21 -0.6748506099852598 2314 22 -0.5272729535786524 2314 23 -0.4940202867503203 2314 24 -0.7708172953807662 2314 25 -0.57917696522032 2315 1 -0.4522487782230584 2315 2 -0.42898825952389075 2315 3 -0.5495802213883216 2315 4 -0.7321083078606065 2315 5 -0.8016957801061793 2315 6 -0.6634088456900535 2315 7 -0.7595345103745146 2315 8 -0.5128816143923061 2315 9 -0.626517355698688 2315 10 -0.8935328471127804 2315 11 -0.8830086269044592 2315 12 -0.8989847850443908 2315 13 -0.906009235339134 2315 14 -1.2492077446019145 2315 15 -1.2039809968289035 2315 16 -1.1006145204937638 2315 17 -1.213909470859953 2315 18 -1.0577859975086987 2315 19 -1.25853219870206 2315 20 -0.9825045589252959 2315 21 -1.2042507231714517 2315 22 -1.2107194731032713 2315 23 -1.122939358623551 2315 24 -1.0264483083971783 2315 25 -1.2620858503363337 2316 1 -1.789620085833949 2316 2 -1.2375741667050058 2316 3 -0.9053693743438345 2316 4 -1.21985967897578 2316 5 -1.1443911840639736 2316 6 -1.0155448988027267 2316 7 -0.9638659514085433 2316 8 -1.1302330368240696 2316 9 -1.4032998054244672 2316 10 -1.5872112845225743 2316 11 -1.3723524159932694 2316 12 -1.133605844090546 2316 13 -1.48673344073379 2316 14 -1.6959619702024789 2316 15 -1.3458842009085714 2316 16 -1.452152414589147 2316 17 -1.6831275343000875 2316 18 -1.4211764205498483 2316 19 -1.4617731621710597 2316 20 -1.3292603881693381 2316 21 -1.5653864234825576 2316 22 -1.2262732111962187 2316 23 -1.4706220937978678 2316 24 -1.2951419804200128 2316 25 -1.493959868104703 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49178.0 3 3.0 4 836.0 5 6099.0 6 7411.0 7 6037.0 8 4874.0 9 4557.0 10 4080.0 11 3926.0 12 3740.0 13 3715.0 14 3433.0 15 3517.0 16 3549.0 17 3793.0 18 4002.0 19 4613.0 20 5542.0 21 6824.0 22 8763.0 23 10765.0 24 12924.0 25 14889.0 26 17008.0 27 19369.0 28 22467.0 29 26892.0 30 33329.0 31 42295.0 32 52830.0 33 67342.0 34 86454.0 35 112098.0 36 150016.0 37 198322.0 38 167904.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.54170319214898 22.86331273652477 14.935665262662761 21.65931880866349 2 17.104967121074218 24.147176230360422 38.5497308666469 20.198125781918467 3 20.432403042110252 27.607900487133076 30.91599085048867 21.043705620268007 4 15.237311189061492 20.303375842426597 37.28638924966508 27.172923718846835 5 12.876797572265176 39.73753526949732 35.76399485129272 11.621672306944788 6 30.382581839379036 39.647740404773835 17.80242987022284 12.167247885624292 7 26.746980558992874 33.062239857643966 23.28020548902502 16.910574094338145 8 24.772284889329775 38.68566110673635 20.6659132978125 15.876140706121378 9 26.79385305557544 15.004738383290892 20.864567460601535 37.33684110053213 10 15.39863780002659 30.38743953447941 34.69672642483867 19.51719624065533 11 34.17789049903017 22.455249549171803 25.637295508080822 17.729564443717212 12 23.352048242877938 25.843023156717766 32.46951583267712 18.335412767727178 13 29.76539889346819 22.75293251383165 26.211781870740996 21.269886721959168 14 20.77167469464699 23.55700888702535 28.415641437332322 27.25567498099533 15 22.867386628214177 32.99730014419684 23.60081336564979 20.534499861939192 16 21.535185052616505 28.971549246801594 28.886582193905554 20.606683506676347 17 21.293067302087277 29.976666018973987 28.397829888630948 20.33243679030779 18 21.20537312211734 27.729768978247755 31.351052841495964 19.713805058138938 19 23.6559524661751 27.210933052439245 28.71485841097123 20.41825607041442 20 23.746885109545286 27.92083831886252 29.10551936430668 19.22675720728552 21 22.961983848589902 27.123494540632485 28.211362574953387 21.703159035824225 22 23.324180413091575 28.74008433640476 28.463792274730782 19.471942975772887 23 22.026323594080772 28.786360273939916 28.962174747485076 20.22514138449424 24 23.25358419426154 27.472118198723994 29.4351367797734 19.83916082724107 25 22.48145124314595 28.2772879356806 29.272605791405105 19.96865502976835 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 367.0 1 367.0 2 502.5 3 638.0 4 638.0 5 638.0 6 1452.5 7 2267.0 8 2267.0 9 2267.0 10 3014.0 11 3761.0 12 3761.0 13 3761.0 14 5503.5 15 7246.0 16 7246.0 17 7246.0 18 12997.0 19 18748.0 20 18748.0 21 18748.0 22 28933.0 23 39118.0 24 39118.0 25 39118.0 26 56016.5 27 72915.0 28 72915.0 29 72915.0 30 91021.5 31 109128.0 32 109128.0 33 109128.0 34 119303.5 35 129479.0 36 129479.0 37 129479.0 38 133670.0 39 137861.0 40 137861.0 41 137861.0 42 141098.0 43 144335.0 44 144335.0 45 144335.0 46 143618.5 47 142902.0 48 142902.0 49 142902.0 50 135025.5 51 127149.0 52 127149.0 53 127149.0 54 110616.5 55 94084.0 56 94084.0 57 94084.0 58 79405.5 59 64727.0 60 64727.0 61 64727.0 62 52486.5 63 40246.0 64 40246.0 65 40246.0 66 31067.5 67 21889.0 68 21889.0 69 21889.0 70 16095.5 71 10302.0 72 10302.0 73 10302.0 74 7221.5 75 4141.0 76 4141.0 77 4141.0 78 2797.0 79 1453.0 80 1453.0 81 1453.0 82 962.0 83 471.0 84 471.0 85 471.0 86 306.5 87 142.0 88 142.0 89 142.0 90 82.0 91 22.0 92 22.0 93 22.0 94 13.5 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3512028334850298 2 0.0 3 0.0 4 0.0 5 0.024373698222935822 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.004261136052960807 25 1.704454421184323E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1173396.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.4238783796297 #Duplication Level Percentage of deduplicated Percentage of total 1 88.43480925515179 66.70096297786321 2 8.170257687035985 12.324650442344737 3 1.772458423916139 4.01057065795203 4 0.6221590668465249 1.8770259916246483 5 0.30292117607534347 1.142374497146055 6 0.1652233968770372 0.7477073634913766 7 0.100303577412339 0.5295699376653019 8 0.07248864683645187 0.4373899906317181 9 0.052437638979510495 0.35595450944149765 >10 0.2618405227194462 3.5191103675332944 >50 0.02088682264854607 1.1053954657728413 >100 0.01972976359916736 2.9591913367593827 >500 0.0025783125934965207 1.228942689631149 >1k 0.0017936087606932319 2.6238581690625007 >5k 1.1210054754332699E-4 0.4372956030803098 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5172 0.44077191331826593 No Hit TATCAACGCAGAGTACTTTTTTTTT 4092 0.34873137457431247 No Hit GGTATCAACGCAGAGTACTTTTTTT 4053 0.34540768845300307 No Hit CTGTAGGACGTGGAATATGGCAAGA 2632 0.22430620182785693 No Hit CTTTAGGACGTGAAATATGGCGAGG 2386 0.20334141244728976 No Hit GTCCTACAGTGGACATTTCTAAATT 2325 0.19814282646267756 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2253 0.192006790546414 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1895 0.1614970564072146 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1530 0.13039076322060072 No Hit CTGAAGGACCTGGAATATGGCGAGA 1444 0.12306160920950812 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1345 0.11462455982464573 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1311 0.11172698730863238 No Hit ATTTAGAAATGTCCACTGTAGGACG 1226 0.10448305601859902 No Hit GTCCTACAGTGTGCATTTCTCATTT 1218 0.10380127425012528 No Hit CTGTAGGACCTGGAATATGGCGAGA 1175 0.10013669724457898 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 8.522272105921615E-5 0.0 5 0.0 0.0 0.0 8.522272105921615E-5 0.0 6 0.0 0.0 0.0 8.522272105921615E-5 0.0 7 0.0 0.0 0.0 8.522272105921615E-5 0.0 8 0.0 0.0 0.0 8.522272105921615E-5 0.0 9 0.0 0.0 0.0 8.522272105921615E-5 0.0 10 0.0 0.0 0.0 8.522272105921615E-5 0.0 11 0.0 0.0 0.0 8.522272105921615E-5 0.0 12 0.0 0.0 0.0 8.522272105921615E-5 0.0 13 0.0 0.0 0.0 8.522272105921615E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACACCGT 50 8.728558E-5 15.19642 6 GGTATCA 1040 0.0 14.756718 1 CCAACGA 50 0.0015008644 13.298001 19 TAGGACC 480 0.0 13.259554 4 CACCTTT 450 0.0 12.030499 14 ACTGATC 120 1.2779674E-7 11.080723 8 GCGCCAC 60 0.0058845123 11.080723 13 CCGTCTC 60 0.0058845123 11.080723 9 TAGAAAT 490 0.0 11.050302 4 TCCAACG 95 1.3615629E-5 10.998346 18 CCACCTT 490 0.0 10.854587 13 GGCGAGG 845 0.0 10.791277 19 AAGTCGT 115 8.834395E-7 10.736601 7 ATTTAGA 515 0.0 10.7353945 1 AATGTCC 510 0.0 10.615147 8 CTAGAGT 90 9.51587E-5 10.554869 4 TTCCACC 490 0.0 10.466923 11 TTTAGAA 565 0.0 10.424101 2 TAAGATT 110 6.044662E-6 10.362962 4 AAATGTC 480 0.0 10.289243 7 >>END_MODULE