FastQCFastQC Report
Thu 26 May 2016
SRR1511778_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511778_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642168
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT30860.48055960434029726No Hit
GTCCTAAAGTGTGTATTTCTCATTT27210.42372089546660685No Hit
CTGTAGGACGTGGAATATGGCAAGA27070.42154078060569816No Hit
GTATCAACGCAGAGTACTTTTTTTT27060.4213850581156333No Hit
CTTTAGGACGTGAAATATGGCGAGG22790.3548915548579188No Hit
GTCCTACAGTGTGCATTTCTCATTT17360.2703342427526753No Hit
GGTATCAACGCAGAGTACTTTTTTT16890.2630152857196248No Hit
TATCAACGCAGAGTACTTTTTTTTT16660.2594336684481319No Hit
CTGTAGGACCTGGAATATGGCGAGA13010.20259495957444157No Hit
CTGAAGGACCTGGAATATGGCGAGA12070.1879570455083405No Hit
ATTTAGAAATGTCCACTGTAGGACG10670.16615589689925378No Hit
TTTCTAAATTTTCCACCTTTTTCAG10370.16148422219730663No Hit
GTCCTTCAGTGTGCATTTCTCATTT10020.15603393504503496No Hit
GAATATGGCAAGAAAACTGAAAATC9450.14715775311133536No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7770.12099637478043129No Hit
GTACTTTTTTTTTTTTTTTTTTTTT7580.11803764746919809No Hit
GGAATATGGCGAGAAAACTGAAAAT7360.11461175268777017No Hit
ATTCCAGGTCCTTCAGTGTGCATTT6960.10838285308517398No Hit
GCCATATTCCACGTCCTACAGTGGA6500.10121961854218833No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTAC400.003841903514.9462291
TAGGACC4450.013.3760724
GTATTAG602.7304678E-413.2855381
AAGACTG604.279139E-412.597575
TATACAG905.757847E-712.597575
GGTATCA4150.012.4852051
GTAAGAC854.1811036E-612.2270543
ATAGCAC701.1460138E-412.1476573
TAGAACG550.003193218712.0249534
GTCATAT751.3279874E-411.95698451
GAACAGT951.1084248E-611.9336126
AACAGTG1201.0806616E-811.8102237
CCTACAC658.388303E-411.6285263
CCTAATA600.00411222711.62484552
TAGGACA4050.011.43112854
CCACCTT3100.011.27685713
TTCAGGA802.4199551E-411.2096722
TCCTAAT802.4199551E-411.2096721
TAGCACT855.6155994E-511.1155034
CTAGGAC600.006113132511.0228743
Produced by FastQC (version 0.11.5)