FastQCFastQC Report
Thu 26 May 2016
SRR1511778_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511778_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642168
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41980.6537230132924718No Hit
GGTATCAACGCAGAGTACTTTTTTT36350.5660512513859302No Hit
TATCAACGCAGAGTACTTTTTTTTT34670.5398898730550261No Hit
CTGTAGGACGTGGAATATGGCAAGA23410.3645463492419429No Hit
CTTTAGGACGTGAAATATGGCGAGG20160.31393653997084875No Hit
GTCCTAAAGTGTGTATTTCTCATTT18630.29011099899091825No Hit
GTCCTACAGTGGACATTTCTAAATT18000.28030048211682923No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15890.24744303671313422No Hit
CTGAAGGACCTGGAATATGGCGAGA13630.2122497539584657No Hit
TTTCTAAATTTTCCACCTTTTTCAG13210.2057094093757397No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12300.1915386627798333No Hit
CTGTAGGACCTGGAATATGGCGAGA12060.1878013230182756No Hit
GAATATGGCAAGAAAACTGAAAATC11340.17658930373360243No Hit
GTCCTACAGTGTGCATTTCTCATTT11100.1728519639720447No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10970.17082757160120093No Hit
ATTTAGAAATGTCCACTGTAGGACG10740.16724595432970812No Hit
GGAATATGGCGAGAAAACTGAAAAT9710.15120653785302288No Hit
ACAGTGGACATTTCTAAATTTTCCA8830.13750295872731125No Hit
GAAATATGGCGAGGAAAACTGAAAA7200.11212019284673169No Hit
GCAGAGTACTTTTTTTTTTTTTTTT6860.10682562818452493No Hit
ATTCCAGGTCCTTCAGTGTGCATTT6830.1063584607143302No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGAC400.00528052414.2476063
GTGGTAT2000.013.8146361
GGTATCA8150.013.7941781
AAAGGCG500.001499774513.2977665
AAATGTC3600.013.1922277
TTTATAC655.4513628E-513.1516363
CCACCTT4200.013.11684413
GGTTTAT751.432422E-512.7031141
TCCACCT4550.012.52536812
CTACACT1001.4404395E-712.3479264
ACCTTTT4750.012.1979515
CACCTTT4450.012.16649514
CTCTAGG550.002999802612.1256991
AGGACGA550.003068935812.08887810
CTGTGCA1103.806781E-812.0888789
TAGAAAT4100.011.8150884
AATGTCC4200.011.7599298
CTATTAT657.821295E-411.725951
GTGCAAA658.023479E-411.69034311
ATGCCTG658.023479E-411.6903435
Produced by FastQC (version 0.11.5)