##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511778_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 642168 Sequences flagged as poor quality 0 Sequence length 25 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.254804038818502 31.0 28.0 31.0 20.0 34.0 2 28.4690548267743 31.0 28.0 31.0 22.0 34.0 3 28.44388695793001 31.0 28.0 33.0 22.0 34.0 4 31.92293605411668 35.0 32.0 37.0 24.0 37.0 5 31.347627100696393 35.0 32.0 37.0 18.0 37.0 6 31.359008857495233 35.0 32.0 37.0 17.0 37.0 7 31.070375664935032 35.0 32.0 37.0 17.0 37.0 8 30.91474972281397 35.0 31.0 37.0 13.0 37.0 9 31.990841960359283 37.0 32.0 39.0 11.0 39.0 10 31.610471091676946 37.0 30.0 39.0 10.0 39.0 11 31.67269624148198 37.0 31.0 39.0 10.0 39.0 12 31.37258007250439 37.0 30.0 39.0 10.0 39.0 13 31.36928498461462 37.0 30.0 39.0 10.0 39.0 14 31.795548828343986 37.0 30.0 40.0 8.0 41.0 15 31.740994568399547 37.0 30.0 40.0 2.0 41.0 16 31.5328699032029 37.0 30.0 40.0 2.0 41.0 17 31.461553674427876 37.0 30.0 40.0 2.0 41.0 18 31.3032679921765 36.0 30.0 40.0 2.0 41.0 19 31.345763102490313 37.0 30.0 40.0 2.0 41.0 20 31.160730525345393 37.0 30.0 40.0 2.0 41.0 21 30.926667164978635 36.0 29.0 40.0 2.0 41.0 22 30.985382329857607 37.0 30.0 40.0 2.0 41.0 23 30.748012981026772 37.0 30.0 40.0 2.0 41.0 24 30.498885026971134 37.0 29.0 40.0 2.0 41.0 25 29.927911387674254 36.0 27.0 39.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.13839108136570744 1101 2 -0.20375384411122255 1101 3 -0.052373772538967245 1101 4 -0.07856708071560448 1101 5 0.08110439996664809 1101 6 0.21998391449765364 1101 7 -0.004155260494009383 1101 8 0.010588137369637707 1101 9 -0.30345601568305725 1101 10 -0.08875801635669234 1101 11 -0.40877539912300165 1101 12 -0.08500219166075595 1101 13 -0.4196551261721453 1101 14 -0.5440376660166599 1101 15 -1.0142548223465049 1101 16 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0.06031902675287526 2313 18 0.2900661049937696 2313 19 0.5077425257126436 2313 20 0.27592118157040346 2313 21 0.7199403137656297 2313 22 0.6349978308867534 2313 23 0.6948607371573594 2313 24 0.4704190859553208 2313 25 0.8109581319559531 2314 1 -0.05590362623179246 2314 2 -0.3727744185734885 2314 3 -0.07112933946108768 2314 4 -0.18590395336206456 2314 5 -0.03299876336794938 2314 6 -0.4410224968328542 2314 7 -0.32030793305757044 2314 8 0.3012674561988433 2314 9 0.46991844305777875 2314 10 0.16405741316717481 2314 11 0.036000733442502764 2314 12 0.05293621723573949 2314 13 0.452929601280168 2314 14 0.4972192898800891 2314 15 0.31818192418987934 2314 16 0.13575632440773333 2314 17 0.3363849400390855 2314 18 0.2558600809516278 2314 19 0.009894612516479384 2314 20 0.5181029833803166 2314 21 0.22303787803558706 2314 22 0.5034931710335293 2314 23 0.37847020994111347 2314 24 0.24749350853872443 2314 25 0.31356945067190267 2315 1 -0.27376040883809694 2315 2 -0.0547282750259761 2315 3 -0.06528574334550896 2315 4 -0.39559712444715345 2315 5 0.038678708640560444 2315 6 -0.2069072663443663 2315 7 -0.3320042953100959 2315 8 -0.2772416817274497 2315 9 -0.4489429153891855 2315 10 -0.37681337206175414 2315 11 -0.39292243094847024 2315 12 -0.23289105624180806 2315 13 -0.29142854307431065 2315 14 -0.40613556243671667 2315 15 -0.46897270988042195 2315 16 -0.10490859384152529 2315 17 -0.7017761038761456 2315 18 -0.27900204777713 2315 19 -0.7368981725107098 2315 20 -0.17141384819390026 2315 21 -0.8792582953144965 2315 22 -0.8605910647827422 2315 23 -0.9385305511706576 2315 24 -0.6755883522420092 2315 25 -0.5795126852094725 2316 1 -2.4169949484105864 2316 2 -1.9689726972572785 2316 3 -1.523162572031314 2316 4 -2.0477221176765212 2316 5 -1.5109143149015374 2316 6 -1.4674583710650957 2316 7 -1.777149876180971 2316 8 -1.3805393481987949 2316 9 -1.8895410944588242 2316 10 -1.7021458715816564 2316 11 -1.6465280995667193 2316 12 -2.5298804645133046 2316 13 -1.992947133995262 2316 14 -2.141684574417038 2316 15 -1.9197120627558455 2316 16 -1.947426185171178 2316 17 -2.206946403704297 2316 18 -2.191427016878432 2316 19 -1.8962320409290427 2316 20 -1.819700509312753 2316 21 -2.2452121171508885 2316 22 -1.9527085733875964 2316 23 -1.8046025512888377 2316 24 -1.764675080328555 2316 25 -1.36696658336934 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33754.0 3 1.0 4 503.0 5 3930.0 6 4660.0 7 3756.0 8 3141.0 9 2778.0 10 2616.0 11 2428.0 12 2337.0 13 2172.0 14 2212.0 15 2245.0 16 2287.0 17 2349.0 18 2607.0 19 2905.0 20 3555.0 21 4280.0 22 5465.0 23 6712.0 24 8004.0 25 9062.0 26 10648.0 27 11918.0 28 13764.0 29 16430.0 30 20463.0 31 25495.0 32 31758.0 33 39571.0 34 49310.0 35 61968.0 36 78724.0 37 95835.0 38 72525.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.192460329860566 24.190816651771485 15.67853281418938 19.938190204178564 2 17.10331252880866 24.920581530066897 40.10056558408392 17.875540357040524 3 21.34752899552765 28.654183951863065 31.181248520636345 18.81703853197294 4 16.248863225822525 21.359519627262646 37.507163234542986 24.88445391237184 5 12.440011775682592 40.862553173593184 35.86567622168813 10.831758829036092 6 29.743462769867072 40.85675399583909 17.66391349304232 11.735869741251511 7 26.44728482266323 34.01773367716859 23.70501177262025 15.829969727547933 8 24.26374406697313 41.20650670852487 20.383606782025886 14.146142442476112 9 26.828026310871923 15.490338976716373 22.016979980316677 35.66465473209503 10 16.06075045782412 31.33370083840989 34.358298762940535 18.247249940825455 11 33.212959848513165 22.544567776656578 27.203940401888605 17.03853197294166 12 24.302986134469485 26.58260766652963 32.7322445216828 16.382161677318084 13 30.651480609435538 23.698471428037525 26.117308866215694 19.532739096311246 14 20.260430292384548 25.899453102614896 28.540039366645487 25.300077238355072 15 22.135017627785874 36.481574914975525 23.225853670690537 18.15755378654807 16 20.15453899914041 30.128564487797586 30.66752002591222 19.04937648714978 17 20.46115658207821 32.11075606383376 29.109827957792977 18.31825939629505 18 20.227012953037065 27.815549250505324 34.19256080203561 17.764876994422004 19 23.88097818639359 27.140872793412317 30.03248371142754 18.945665308766554 20 23.851702358261388 28.89446375403321 30.341437131716308 16.912396755989086 21 21.932578390701497 27.607884541116967 29.36614717643981 21.093389891741726 22 23.286429719325785 30.27323068106788 29.60829564849074 16.832043951115597 23 20.465672534290093 30.59526479052211 30.850493951738482 18.088568723449317 24 23.14238906659275 28.176166642269155 31.273145192184902 17.408299098953194 25 21.577564714474583 29.93240076179561 30.81643871453999 17.67359580918982 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 262.0 1 262.0 2 361.5 3 461.0 4 461.0 5 461.0 6 1105.0 7 1749.0 8 1749.0 9 1749.0 10 2281.5 11 2814.0 12 2814.0 13 2814.0 14 3753.0 15 4692.0 16 4692.0 17 4692.0 18 8176.0 19 11660.0 20 11660.0 21 11660.0 22 18727.0 23 25794.0 24 25794.0 25 25794.0 26 37753.0 27 49712.0 28 49712.0 29 49712.0 30 61332.0 31 72952.0 32 72952.0 33 72952.0 34 77332.0 35 81712.0 36 81712.0 37 81712.0 38 81048.0 39 80384.0 40 80384.0 41 80384.0 42 79767.5 43 79151.0 44 79151.0 45 79151.0 46 76451.5 47 73752.0 48 73752.0 49 73752.0 50 67378.0 51 61004.0 52 61004.0 53 61004.0 54 50402.0 55 39800.0 56 39800.0 57 39800.0 58 32831.5 59 25863.0 60 25863.0 61 25863.0 62 20737.5 63 15612.0 64 15612.0 65 15612.0 66 12057.0 67 8502.0 68 8502.0 69 8502.0 70 6192.5 71 3883.0 72 3883.0 73 3883.0 74 2724.0 75 1565.0 76 1565.0 77 1565.0 78 1074.5 79 584.0 80 584.0 81 584.0 82 385.5 83 187.0 84 187.0 85 187.0 86 122.5 87 58.0 88 58.0 89 58.0 90 34.5 91 11.0 92 11.0 93 11.0 94 7.5 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.333246128738897 2 0.0 3 0.0 4 0.0 5 0.026005655840839158 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 3.1144498012981023E-4 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.004048784741687533 25 1.5572249006490512E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 642168.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.87823780664155 #Duplication Level Percentage of deduplicated Percentage of total 1 90.69109105717476 66.09406901008565 2 6.390543366738881 9.314630783897037 3 1.3813376836211544 3.0200836859465365 4 0.48756589259393984 1.421317722674744 5 0.26085748728960867 0.9505416996167536 6 0.16046969800932243 0.7016849287370012 7 0.10746650089367464 0.5482378445863813 8 0.06914561199386304 0.40313682833396136 9 0.05128706431698757 0.33639397827281453 >10 0.32696400116234065 4.410438703489347 >50 0.034505886030579976 1.7677595512794746 >100 0.03216279272763645 4.802339563030315 >500 0.0031949794099668267 1.5035543526210762 >1k 0.0034079780372979485 4.725811347428871 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4198 0.6537230132924718 No Hit GGTATCAACGCAGAGTACTTTTTTT 3635 0.5660512513859302 No Hit TATCAACGCAGAGTACTTTTTTTTT 3467 0.5398898730550261 No Hit CTGTAGGACGTGGAATATGGCAAGA 2341 0.3645463492419429 No Hit CTTTAGGACGTGAAATATGGCGAGG 2016 0.31393653997084875 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1863 0.29011099899091825 No Hit GTCCTACAGTGGACATTTCTAAATT 1800 0.28030048211682923 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1589 0.24744303671313422 No Hit CTGAAGGACCTGGAATATGGCGAGA 1363 0.2122497539584657 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1321 0.2057094093757397 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1230 0.1915386627798333 No Hit CTGTAGGACCTGGAATATGGCGAGA 1206 0.1878013230182756 No Hit GAATATGGCAAGAAAACTGAAAATC 1134 0.17658930373360243 No Hit GTCCTACAGTGTGCATTTCTCATTT 1110 0.1728519639720447 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1097 0.17082757160120093 No Hit ATTTAGAAATGTCCACTGTAGGACG 1074 0.16724595432970812 No Hit GGAATATGGCGAGAAAACTGAAAAT 971 0.15120653785302288 No Hit ACAGTGGACATTTCTAAATTTTCCA 883 0.13750295872731125 No Hit GAAATATGGCGAGGAAAACTGAAAA 720 0.11212019284673169 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 686 0.10682562818452493 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 683 0.1063584607143302 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 1.5572249006490514E-4 0.0 7 0.0 0.0 0.0 1.5572249006490514E-4 0.0 8 0.0 0.0 0.0 1.5572249006490514E-4 0.0 9 0.0 0.0 0.0 1.5572249006490514E-4 0.0 10 0.0 0.0 0.0 1.5572249006490514E-4 0.0 11 0.0 0.0 0.0 1.5572249006490514E-4 0.0 12 0.0 0.0 0.0 1.5572249006490514E-4 0.0 13 0.0 0.0 0.0 1.5572249006490514E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTAGAC 40 0.005280524 14.247606 3 GTGGTAT 200 0.0 13.814636 1 GGTATCA 815 0.0 13.794178 1 AAAGGCG 50 0.0014997745 13.297766 5 AAATGTC 360 0.0 13.192227 7 TTTATAC 65 5.4513628E-5 13.151636 3 CCACCTT 420 0.0 13.116844 13 GGTTTAT 75 1.432422E-5 12.703114 1 TCCACCT 455 0.0 12.525368 12 CTACACT 100 1.4404395E-7 12.347926 4 ACCTTTT 475 0.0 12.19795 15 CACCTTT 445 0.0 12.166495 14 CTCTAGG 55 0.0029998026 12.125699 1 AGGACGA 55 0.0030689358 12.088878 10 CTGTGCA 110 3.806781E-8 12.088878 9 TAGAAAT 410 0.0 11.815088 4 AATGTCC 420 0.0 11.759929 8 CTATTAT 65 7.821295E-4 11.72595 1 GTGCAAA 65 8.023479E-4 11.690343 11 ATGCCTG 65 8.023479E-4 11.690343 5 >>END_MODULE