FastQCFastQC Report
Thu 26 May 2016
SRR1511777_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511777_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1781806
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA59830.33578290790355403No Hit
GTCCTACAGTGGACATTTCTAAATT59630.33466045125002386No Hit
GTATCAACGCAGAGTACTTTTTTTT56840.3190021809332778No Hit
GTCCTAAAGTGTGTATTTCTCATTT56370.3163644077974819No Hit
CTTTAGGACGTGAAATATGGCGAGG47260.2652365072291821No Hit
GGTATCAACGCAGAGTACTTTTTTT35440.1988993190055483No Hit
TATCAACGCAGAGTACTTTTTTTTT31960.1793685732341231No Hit
CTGAAGGACCTGGAATATGGCGAGA27950.15686331733084297No Hit
GTCCTACAGTGTGCATTTCTCATTT24970.14013871319324328No Hit
ATTTAGAAATGTCCACTGTAGGACG23130.12981211198076556No Hit
GTCCTTCAGTGTGCATTTCTCATTT21650.12150593274464223No Hit
CTGTAGGACCTGGAATATGGCGAGA19630.1101691205439874No Hit
TTTCTAAATTTTCCACCTTTTTCAG19000.10663338208536732No Hit
GAATATGGCAAGAAAACTGAAAATC18380.10315376645942376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG755.560396E-714.6336731
TCCAACG2500.014.36012518
GGTATCA11850.013.9769261
CCAACGA1800.013.12145919
TAGGACC8550.013.037494
CCAACGT1102.9849616E-912.88288819
CCGTAGG706.897375E-512.8282191
AATGTCC8700.011.61829958
GTCCTAA15000.011.5073881
GTGTAGG1851.8189894E-1211.3258151
TAGACCC1105.344209E-711.1642284
GCCGGTT1950.011.14220811
CGTAGGA905.781898E-511.0861162
CCCGTTT1451.3369572E-911.07538912
ACTGTTC5900.011.0477838
GGACGTA600.006127689511.0210986
GATATAC5850.010.915561
TAGAAAT9800.010.8925714
TCGAACT1658.913048E-1110.87888219
TGGTATC3050.010.7953332
Produced by FastQC (version 0.11.5)