FastQCFastQC Report
Thu 26 May 2016
SRR1511777_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511777_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1781806
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT72170.40503848342636634No Hit
CTGTAGGACGTGGAATATGGCAAGA59820.33572678507087755No Hit
GTCCTACAGTGGACATTTCTAAATT59050.3314053269547863No Hit
GTCCTAAAGTGTGTATTTCTCATTT57410.32220118239583884No Hit
CTTTAGGACGTGAAATATGGCGAGG51270.28774176313246225No Hit
GGTATCAACGCAGAGTACTTTTTTT49890.27999681222310396No Hit
TATCAACGCAGAGTACTTTTTTTTT48790.2738233006286879No Hit
CTGAAGGACCTGGAATATGGCGAGA30120.16904197202164545No Hit
GTCCTACAGTGTGCATTTCTCATTT25730.14440404847665794No Hit
TTTCTAAATTTTCCACCTTTTTCAG24390.13688358889800573No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23920.1342458157622098No Hit
ATTTAGAAATGTCCACTGTAGGACG22970.12891414665794143No Hit
GTCCTTCAGTGTGCATTTCTCATTT21940.123133494892261No Hit
CTGTAGGACCTGGAATATGGCGAGA21250.11926101943758186No Hit
GAATATGGCAAGAAAACTGAAAATC20170.11319975350851888No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19120.10730685607748543No Hit
GGAATATGGCGAGAAAACTGAAAAT19100.10719461041213241No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACCG400.005277280714.2509055
CCAACGA1051.376975E-913.56810119
TCCAACG1550.013.48094418
TAGGACC7850.013.4339744
GGTATCA12750.013.232161
GTCCCGC751.48260315E-512.66356212
GTGGTAT4150.012.6322951
TGGTATC3750.012.1607732
CGCCCTA951.0431304E-611.99705816
TAAGACG658.020157E-411.693054
ACCGTGC658.0428494E-411.6891138
GTATTAG1850.011.3349241
CCACCTT9100.011.2719613
TGTAGGA30350.011.0188222
GGCGAGG13650.010.92406119
TACACGG700.001490797310.8578335
AACACCG700.001490797310.8578335
GCGCATT700.001494991810.854176511
AGGACCT19700.010.7544915
ACCTTTT9550.010.74082215
Produced by FastQC (version 0.11.5)