##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511777_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1781806 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.123889469448415 31.0 30.0 34.0 26.0 34.0 2 30.34901386570704 31.0 30.0 34.0 26.0 34.0 3 30.425701226732876 31.0 30.0 34.0 26.0 34.0 4 34.02165050516162 37.0 35.0 37.0 30.0 37.0 5 33.60351968732847 37.0 35.0 37.0 28.0 37.0 6 33.53889761287144 37.0 35.0 37.0 28.0 37.0 7 33.33166966549669 36.0 35.0 37.0 27.0 37.0 8 33.22165432151424 36.0 35.0 37.0 27.0 37.0 9 34.60404948686894 38.0 35.0 39.0 27.0 39.0 10 34.35367598941748 38.0 34.0 39.0 27.0 39.0 11 34.349425807298886 38.0 35.0 39.0 27.0 39.0 12 34.14417506731934 38.0 34.0 39.0 26.0 39.0 13 34.13536827241574 38.0 34.0 39.0 26.0 39.0 14 34.98599061850729 39.0 34.0 40.0 25.0 41.0 15 34.91748091543075 38.0 34.0 40.0 25.0 41.0 16 34.79276980771195 38.0 34.0 40.0 25.0 41.0 17 34.73226546548839 38.0 34.0 40.0 25.0 41.0 18 34.63652720891051 38.0 34.0 40.0 25.0 41.0 19 34.66467280949778 39.0 34.0 40.0 24.0 41.0 20 34.51105283066731 39.0 34.0 40.0 24.0 41.0 21 34.36740587920346 38.0 34.0 40.0 22.0 41.0 22 34.449591032918285 39.0 34.0 40.0 22.0 41.0 23 34.309734617573405 39.0 34.0 40.0 19.0 41.0 24 34.14598110007487 39.0 34.0 40.0 18.0 41.0 25 33.67537655614584 38.0 33.0 40.0 17.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.48373914443191524 1101 2 0.5088760078937042 1101 3 0.5820819330247424 1101 4 0.5445474361144633 1101 5 0.616051933535374 1101 6 0.697280973863819 1101 7 0.6588194545735462 1101 8 0.5207033930019307 1101 9 0.6399182026993344 1101 10 0.8331420059741603 1101 11 0.5024311464421842 1101 12 0.6527532008275827 1101 13 0.4276425154565473 1101 14 0.5322501721908424 1101 15 0.48620075659649586 1101 16 0.7259645452460362 1101 17 0.6866156198856217 1101 18 0.6214482228073095 1101 19 0.6084093353965301 1101 20 0.6032560419202753 1101 21 0.6766340513395832 1101 22 0.5667542844309352 1101 23 0.4439783211736952 1101 24 0.45000989556344706 1101 25 0.3450983089485433 1102 1 0.7435925272479622 1102 2 0.7549956332266845 1102 3 0.7367242663518887 1102 4 0.7816787405029331 1102 5 0.8656442901335666 1102 6 0.8851788142980794 1102 7 1.0518200237058295 1102 8 0.9625750763979326 1102 9 1.0390278211630388 1102 10 1.1885302290970614 1102 11 1.0503493322353847 1102 12 1.1213203254675506 1102 13 1.4348776656812277 1102 14 1.7076264621013237 1102 15 1.552070616720556 1102 16 1.5841997101951648 1102 17 1.595643440787562 1102 18 1.649653561127323 1102 19 1.7300874497953203 1102 20 1.7625887551192179 1102 21 1.7446927340640173 1102 22 1.4943365221259342 1102 23 1.6066832552113226 1102 24 1.6690691691867556 1102 25 1.8695258636579055 1103 1 0.413451575366107 1103 2 0.46294154519380015 1103 3 0.3535219665775067 1103 4 0.6089815067576225 1103 5 0.5834929882592021 1103 6 0.4965023309272212 1103 7 0.5751837208803678 1103 8 0.3640448106061953 1103 9 0.6721762672154625 1103 10 0.2997989472021203 1103 11 0.5984485792910661 1103 12 0.5286900341031711 1103 13 0.3741495633604117 1103 14 0.4518420684690412 1103 15 0.5764011614178841 1103 16 0.2644043417412263 1103 17 0.18273152280142568 1103 18 0.4674793320543813 1103 19 0.5992096782182443 1103 20 0.7686128135608996 1103 21 0.7590297915974276 1103 22 0.9070960120335556 1103 23 0.8557746704871363 1103 24 0.9199947603494536 1103 25 0.7358968282505032 1104 1 0.3849570842052614 1104 2 0.4235978483001155 1104 3 0.48937201314442547 1104 4 0.37271416187927997 1104 5 0.45882175438563877 1104 6 0.4961852600384873 1104 7 0.4028271044945626 1104 8 0.6350025236842498 1104 9 0.8577339787035854 1104 10 0.5669187877677473 1104 11 0.5465623897004122 1104 12 0.5936589905088283 1104 13 0.7967347337378641 1104 14 0.9156611307586431 1104 15 0.9281452959122021 1104 16 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15 0.36229998115847906 1107 16 0.213852958080345 1107 17 0.31844427000576303 1107 18 0.552007029984118 1107 19 0.5525103225008863 1107 20 0.3844582979993305 1107 21 0.4948186885067116 1107 22 0.4186554518206762 1107 23 0.49166650373636855 1107 24 0.40642449241598655 1107 25 0.32178338349534386 1108 1 0.2981939513657075 1108 2 0.27879401486127264 1108 3 0.28760778286750366 1108 4 0.4105339511417654 1108 5 0.4092152218712073 1108 6 0.42034421387197085 1108 7 0.3653234008280535 1108 8 0.4406790074181046 1108 9 0.48818294727492884 1108 10 0.36679140822663925 1108 11 0.6187909024526945 1108 12 0.6364469363630718 1108 13 0.6654162343704755 1108 14 0.8118961377405896 1108 15 0.9388233763214231 1108 16 0.542517703115351 1108 17 0.6300925825256769 1108 18 0.5908951639626636 1108 19 0.6502709505496114 1108 20 0.6232982094183797 1108 21 0.6406981209071461 1108 22 0.7782639883927445 1108 23 0.8444805524181618 1108 24 0.73749004886335 1108 25 0.6474816747483985 1109 1 0.16854645290355563 1109 2 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-0.9694453991945693 2315 8 -0.9131994959332346 2315 9 -0.9896280767924992 2315 10 -0.9525849881646664 2315 11 -1.035758434349816 2315 12 -0.835515223200268 2315 13 -1.007940478206237 2315 14 -1.1787328393232457 2315 15 -1.346909534677188 2315 16 -1.1986446600736542 2315 17 -1.1263581089531556 2315 18 -1.3208661980668097 2315 19 -1.1934757617442884 2315 20 -1.1791976190835598 2315 21 -1.1382964773563842 2315 22 -1.3289370071105253 2315 23 -1.288150743715775 2315 24 -1.1948123352538431 2315 25 -0.8386239843482954 2316 1 -1.8042597311504025 2316 2 -1.3751792069323336 2316 3 -1.2211520623629255 2316 4 -1.3084700903762325 2316 5 -1.2552142631033334 2316 6 -1.2399769511540413 2316 7 -1.3078354583118283 2316 8 -1.2681825642911555 2316 9 -1.4074814889488394 2316 10 -1.4702048010556723 2316 11 -1.2139499053938394 2316 12 -1.28560016838766 2316 13 -1.3250879269808493 2316 14 -1.4487466142185 2316 15 -1.4318656271985688 2316 16 -1.4744743105356903 2316 17 -1.5620764123452204 2316 18 -1.5020869981586387 2316 19 -1.3535010157189191 2316 20 -1.463956271439642 2316 21 -1.8256298872169126 2316 22 -1.7039045787112812 2316 23 -1.6806853244424005 2316 24 -1.5403234412631406 2316 25 -1.545857526218235 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 43464.0 3 2.0 4 762.0 5 6294.0 6 7845.0 7 6211.0 8 5104.0 9 4652.0 10 4222.0 11 3992.0 12 3855.0 13 3706.0 14 3568.0 15 3550.0 16 3625.0 17 3789.0 18 4155.0 19 4741.0 20 5672.0 21 7443.0 22 9388.0 23 11734.0 24 13915.0 25 16279.0 26 19046.0 27 21785.0 28 25323.0 29 30218.0 30 38292.0 31 48204.0 32 62462.0 33 79358.0 34 103644.0 35 139359.0 36 202853.0 37 333037.0 38 500257.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.4143661971831 21.713408450704225 14.542704225352113 23.329521126760564 2 14.650360364708618 22.440153417375406 42.01198110231978 20.8975051155962 3 18.97484911376435 26.93362801562011 31.076559400967334 23.01496346964821 4 12.890628946136673 19.112855159315885 39.29266149064489 28.703854403902557 5 12.031931107848887 39.50868309442076 36.67782870712134 11.78155709060901 6 29.702111228719623 39.646179213674216 18.59764755534553 12.054062002260627 7 26.434247050464528 32.64373338062617 23.616936972936447 17.305082595972852 8 23.562497825240232 39.78401689072773 21.121379095142792 15.53210618888925 9 26.23377629214404 15.139470851484393 21.459463039186083 37.167289817185484 10 14.530257502780886 30.36088103867649 35.726785070877526 19.382076387665098 11 33.17841560753528 22.45036777292253 26.694095765756764 17.67712085378543 12 23.148872548414364 25.454342391932677 33.46559614234098 17.931188917311985 13 29.00063194309594 23.17901050956165 26.518262930981262 21.302094616361153 14 19.548199972387568 24.228563603445043 28.89977921277625 27.32345721139114 15 21.60599975530445 34.271127159746904 23.85534676614626 20.267526318802386 16 20.11016918237405 29.420054383055383 30.43649557611523 20.03328085845533 17 20.094443502828028 30.481545128930982 29.384568241435936 20.03944312680505 18 19.746716803232687 28.211808283758 33.03962285329442 19.001852059714892 19 22.79310991207797 27.4102792335417 29.133362442375883 20.66324841200445 20 22.813706991670248 28.551986018679926 30.023807305621375 18.610499684028454 21 21.553356538253883 27.681857620863326 28.670910301121445 22.093875539761342 22 22.019119926636233 30.050914633804126 29.04833635087097 18.88162908868867 23 20.52591584044503 29.561355164366944 29.956123169413505 19.956605825774524 24 22.192170011573126 27.75366865838176 30.784401985952854 19.26975934409226 25 21.38686409953059 28.83656114813975 30.171668713281598 19.604906039048068 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 191.0 1 191.0 2 440.0 3 689.0 4 689.0 5 689.0 6 1797.0 7 2905.0 8 2905.0 9 2905.0 10 4075.0 11 5245.0 12 5245.0 13 5245.0 14 8018.5 15 10792.0 16 10792.0 17 10792.0 18 19414.5 19 28037.0 20 28037.0 21 28037.0 22 45338.5 23 62640.0 24 62640.0 25 62640.0 26 89913.5 27 117187.0 28 117187.0 29 117187.0 30 150377.5 31 183568.0 32 183568.0 33 183568.0 34 199291.5 35 215015.0 36 215015.0 37 215015.0 38 220015.0 39 225015.0 40 225015.0 41 225015.0 42 227023.5 43 229032.0 44 229032.0 45 229032.0 46 225642.5 47 222253.0 48 222253.0 49 222253.0 50 204854.5 51 187456.0 52 187456.0 53 187456.0 54 156199.5 55 124943.0 56 124943.0 57 124943.0 58 103162.5 59 81382.0 60 81382.0 61 81382.0 62 64025.5 63 46669.0 64 46669.0 65 46669.0 66 35023.5 67 23378.0 68 23378.0 69 23378.0 70 16680.0 71 9982.0 72 9982.0 73 9982.0 74 6855.5 75 3729.0 76 3729.0 77 3729.0 78 2478.0 79 1227.0 80 1227.0 81 1227.0 82 782.5 83 338.0 84 338.0 85 338.0 86 222.5 87 107.0 88 107.0 89 107.0 90 63.5 91 20.0 92 20.0 93 20.0 94 13.0 95 6.0 96 6.0 97 6.0 98 3.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.381971999196321 2 0.0 3 0.0 4 0.0 5 0.02643385419063579 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 5.612283267650911E-5 17 0.0 18 3.3673699605905465E-4 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005163300606238839 25 1.1224566535301823E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1781806.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.09055679328951 #Duplication Level Percentage of deduplicated Percentage of total 1 88.50462800323989 64.68852539539766 2 8.356634002976087 12.21582064390516 3 1.6274809373551566 3.5686046364525934 4 0.5353984874548648 1.565302942174444 5 0.25145356319076 0.9189440470634627 6 0.147443963060628 0.6466056813546307 7 0.10312656048712784 0.5276304408326717 8 0.06774154875649616 0.39610140133216587 9 0.04937449849939762 0.3247928628039369 >10 0.30260138431736394 4.046264735901049 >50 0.026399738899215968 1.3527429321202606 >100 0.021931056238288043 3.2854909244911026 >500 0.0028931277623939957 1.4716525255266555 >1k 0.0025124530568158385 3.3236553880409345 >5k 3.806747055781573E-4 1.667865442603226 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7217 0.40503848342636634 No Hit CTGTAGGACGTGGAATATGGCAAGA 5982 0.33572678507087755 No Hit GTCCTACAGTGGACATTTCTAAATT 5905 0.3314053269547863 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5741 0.32220118239583884 No Hit CTTTAGGACGTGAAATATGGCGAGG 5127 0.28774176313246225 No Hit GGTATCAACGCAGAGTACTTTTTTT 4989 0.27999681222310396 No Hit TATCAACGCAGAGTACTTTTTTTTT 4879 0.2738233006286879 No Hit CTGAAGGACCTGGAATATGGCGAGA 3012 0.16904197202164545 No Hit GTCCTACAGTGTGCATTTCTCATTT 2573 0.14440404847665794 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2439 0.13688358889800573 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2392 0.1342458157622098 No Hit ATTTAGAAATGTCCACTGTAGGACG 2297 0.12891414665794143 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2194 0.123133494892261 No Hit CTGTAGGACCTGGAATATGGCGAGA 2125 0.11926101943758186 No Hit GAATATGGCAAGAAAACTGAAAATC 2017 0.11319975350851888 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1912 0.10730685607748543 No Hit GGAATATGGCGAGAAAACTGAAAAT 1910 0.10719461041213241 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.1224566535301823E-4 0.0 3 0.0 0.0 0.0 1.1224566535301823E-4 0.0 4 0.0 0.0 0.0 1.1224566535301823E-4 0.0 5 0.0 0.0 0.0 1.1224566535301823E-4 0.0 6 0.0 0.0 0.0 1.1224566535301823E-4 0.0 7 0.0 0.0 0.0 1.1224566535301823E-4 0.0 8 0.0 0.0 0.0 1.1224566535301823E-4 0.0 9 0.0 0.0 0.0 1.1224566535301823E-4 0.0 10 0.0 5.612283267650911E-5 0.0 1.1224566535301823E-4 0.0 11 0.0 5.612283267650911E-5 0.0 1.1224566535301823E-4 0.0 12 0.0 1.1224566535301823E-4 0.0 1.1224566535301823E-4 0.0 13 0.0 1.1224566535301823E-4 0.0 1.1224566535301823E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATACCG 40 0.0052772807 14.250905 5 CCAACGA 105 1.376975E-9 13.568101 19 TCCAACG 155 0.0 13.480944 18 TAGGACC 785 0.0 13.433974 4 GGTATCA 1275 0.0 13.23216 1 GTCCCGC 75 1.48260315E-5 12.663562 12 GTGGTAT 415 0.0 12.632295 1 TGGTATC 375 0.0 12.160773 2 CGCCCTA 95 1.0431304E-6 11.997058 16 TAAGACG 65 8.020157E-4 11.69305 4 ACCGTGC 65 8.0428494E-4 11.689113 8 GTATTAG 185 0.0 11.334924 1 CCACCTT 910 0.0 11.27196 13 TGTAGGA 3035 0.0 11.018822 2 GGCGAGG 1365 0.0 10.924061 19 TACACGG 70 0.0014907973 10.857833 5 AACACCG 70 0.0014907973 10.857833 5 GCGCATT 70 0.0014949918 10.8541765 11 AGGACCT 1970 0.0 10.754491 5 ACCTTTT 955 0.0 10.740822 15 >>END_MODULE