FastQCFastQC Report
Thu 26 May 2016
SRR1511776_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511776_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1935374
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT73420.3793582015672423No Hit
CTGTAGGACGTGGAATATGGCAAGA66100.34153605453002883No Hit
GTCCTAAAGTGTGTATTTCTCATTT55670.2876446619619774No Hit
CTTTAGGACGTGAAATATGGCGAGG48410.25013253252342954No Hit
GTATCAACGCAGAGTACTTTTTTTT42000.217012319065979No Hit
GTCCTACAGTGTGCATTTCTCATTT38130.19701618395204235No Hit
CTGAAGGACCTGGAATATGGCGAGA27390.14152303379088488No Hit
CTGTAGGACCTGGAATATGGCGAGA27370.14141969459132964No Hit
GGTATCAACGCAGAGTACTTTTTTT26460.13671776101156677No Hit
TATCAACGCAGAGTACTTTTTTTTT25410.1312924530349173No Hit
GTCCTTCAGTGTGCATTTCTCATTT22600.11677329549740774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG1003.1650416E-1014.9625811
CCAACGT1350.014.69573219
TCCAACG3100.014.6280118
TAGGACC11550.013.2503554
CCAACGA2200.012.45320119
GGTATCA8900.012.4407971
GGCGAGG14950.012.32250819
CGTTAGG655.3254067E-412.2769891
CCCGTTT1850.012.25558312
GCCGGTT1950.012.11155411
ATCCCGT1800.012.07118210
CCGTTTC1950.011.62709213
GGCCTAC1301.5905243E-811.5096771
AGGACGT36100.011.4358585
GACGTGG18550.011.3567857
GGACGTG35650.011.3417156
GAATACG752.1848889E-411.33641413
CTAGGAC3200.011.21832753
TCCCGTT1950.011.14263111
GGCGAAG855.634749E-511.11441919
Produced by FastQC (version 0.11.5)