FastQCFastQC Report
Thu 26 May 2016
SRR1511776_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511776_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1935374
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA68130.35202498328488446No Hit
GTCCTACAGTGGACATTTCTAAATT61790.31926645702587714No Hit
GTATCAACGCAGAGTACTTTTTTTT55610.2873346443633117No Hit
CTTTAGGACGTGAAATATGGCGAGG48960.25297436051119837No Hit
GTCCTAAAGTGTGTATTTCTCATTT48540.2508042373205386No Hit
TATCAACGCAGAGTACTTTTTTTTT38870.2008397343355858No Hit
GGTATCAACGCAGAGTACTTTTTTT38570.19928964634225738No Hit
GTCCTACAGTGTGCATTTCTCATTT31970.1651877104890321No Hit
CTGAAGGACCTGGAATATGGCGAGA30580.15800563611994373No Hit
CTGTAGGACCTGGAATATGGCGAGA28220.14581161057242684No Hit
TTTCTAAATTTTCCACCTTTTTCAG22370.11558489470252262No Hit
GAATATGGCAAGAAAACTGAAAATC21600.11160633551964634No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20220.10447593075033561No Hit
ATTTAGAAATGTCCACTGTAGGACG19990.10328752995545046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGCG707.284265E-613.56790357
GGTATCA12750.013.0141261
TCCAACG3000.012.98029618
TAGGACC11750.012.9354844
TCGAATA550.00307488912.087769511
ACGGGAT802.8748418E-511.87191615
CCAACGA1850.011.80804819
GTCGAGG907.4825493E-611.60839619
CCACCTT7400.011.55105313
GGCGAGG14600.011.44937619
CCAACGT1251.8388164E-811.39733419
TGTAGAA10250.011.214042
TCTATAC2300.011.1515833
TGTAGGA38350.011.0724242
ACCTTTT8200.010.88741515
CTGTAGG37150.010.85819151
CACCTTT8050.010.85432314
GGACGTC700.001494924710.8543226
TGGTATC4300.010.8250082
GAACAGT5400.010.7286956
Produced by FastQC (version 0.11.5)