FastQCFastQC Report
Thu 26 May 2016
SRR1511775_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511775_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34297
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT1680.48983876140770327No Hit
CTGTAGGACGTGGAATATGGCAAGA1670.4869230544945622No Hit
GTATCAACGCAGAGTACTTTTTTTT1480.4315246231448815No Hit
GGTATCAACGCAGAGTACTTTTTTT1460.42569320931859933No Hit
GTCCTACAGTGTGCATTTCTCATTT1140.33239058809808436No Hit
TATCAACGCAGAGTACTTTTTTTTT1030.3003178120535324No Hit
GTCCTAAAGTGTGTATTTCTCATTT900.2624136221826982No Hit
CTGTAGGACCTGGAATATGGCGAGA770.224509432311864No Hit
ACGCAGAGTACTTTTTTTTTTTTTT550.16036388022276No Hit
CTTTAGGACGTGAAATATGGCGAGG520.15161675948333675No Hit
GTCCTTCAGTGTGCATTTCTCATTT490.14286963874391345No Hit
GTCCACTGTAGGACGTGGAATATGG400.11662827652564364No Hit
GTGGTATCAACGCAGAGTACTTTTT390.11371256961250256No Hit
CTGAAGGACCTGGAATATGGCGAGA380.11079686269936145No Hit
GACCTGGAATATGGCGAGAAAACTG370.10788115578622037No Hit
GTACTTTTTTTTTTTTTTTTTTTTT360.10496544887307928No Hit
GAATATGGCAAGAAAACTGAAAATC360.10496544887307928No Hit
ACCTGGAATATGGCGAGAAAACTGA350.1020497419599382No Hit
ATTCCAGGTCCTTCAGTGTGCATTT350.1020497419599382No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCATTT500.001511617813.224380512
GTGCATT500.001511617813.224380511
TCTAAAT500.001511617813.224380518
GTGTGCA500.001511617813.22438059
TGTGCAT500.001511617813.224380510
CTAAATT550.00309386912.02216319
TGTAGGA1009.565738E-711.9824082
CATTTCT1052.621E-711.69502914
GACGTGG658.054299E-411.6258297
ATTTCTC752.0702234E-411.33518215
TGGCAAG855.277712E-511.11292518
GGCAAGA855.277712E-511.11292519
GGACGTG855.277712E-511.1129256
TTCTAAA600.005925704311.02031617
TCCTACA1001.2706174E-510.9838732
CCTACAG1053.3784945E-610.7954123
ATGGCAA803.7654926E-410.62673317
TAGGACG909.43786E-510.495544
AGGACGT909.43786E-510.495545
CTGTAGG1052.1810793E-510.4608331
Produced by FastQC (version 0.11.5)