FastQCFastQC Report
Thu 26 May 2016
SRR1511775_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511775_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34297
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT3070.8951220223343149No Hit
GGTATCAACGCAGAGTACTTTTTTT2770.8076508149400823No Hit
TATCAACGCAGAGTACTTTTTTTTT1870.545237192757384No Hit
CTGTAGGACGTGGAATATGGCAAGA1690.49275446832084435No Hit
ACGCAGAGTACTTTTTTTTTTTTTT1480.4315246231448815No Hit
GTCCTACAGTGGACATTTCTAAATT1330.3877890194477651No Hit
GTCCTAAAGTGTGTATTTCTCATTT1100.32072776044552No Hit
CTGTAGGACCTGGAATATGGCGAGA1000.2915706913141091No Hit
GTCCTACAGTGTGCATTTCTCATTT890.25949791526955707No Hit
CTTTAGGACGTGAAATATGGCGAGG670.1953523631804531No Hit
GAGTACTTTTTTTTTTTTTTTTTTT660.19243665626731202No Hit
GTGGTATCAACGCAGAGTACTTTTT510.14870105257019564No Hit
GAATATGGCAAGAAAACTGAAAATC500.14578534565705456No Hit
CTGAAGGACCTGGAATATGGCGAGA460.13412251800449018No Hit
ATTCCAGGTCCTTCAGTGTGCATTT460.13412251800449018No Hit
ACAGTGGACATTTCTAAATTTTCCA440.128291104178208No Hit
GTGGTATCAACGCAGAGTACATGGG420.12245969035192582No Hit
GTGTATATCAATGAGTTACAATGAA400.11662827652564364No Hit
GGAATATGGCGAGAAAACTGAAAAT380.11079686269936145No Hit
GTACTTTTTTTTTTTTTTTTTTTTT350.1020497419599382No Hit
GATATACACTGTTCTACAAATCCCG350.1020497419599382No Hit
ACCTGGAATATGGCGAGAAAACTGA350.1020497419599382No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGA551.8748458E-413.8171212
CTGTAGG550.00293372412.10765651
GTAGGAC600.005682598311.0824813
GTATCAA1002.1825472E-510.4644751
TAGGACG900.00105691719.499274
TATCAAC1106.331089E-59.499272
ATCAACG1106.331089E-59.499273
TCAACGC1151.0292436E-49.0862584
AGGACGT950.00173183338.9993085
CAACGCA1201.6356938E-48.7076645
TGGAATA1000.00276014538.54934311
GGAATAT1000.00276014538.54934312
ATATGGC1451.5230169E-58.51658615
AACGCAG1252.5458663E-48.3593586
TTTCTCA1200.00159017027.91605816
TTCTCAT1200.00159017027.91605817
ATTTCTC1100.0065193837.772130515
CTCATTT1250.00237677577.599416319
AATATGG1604.4631714E-47.12445214
TCTCATT1350.00504343357.03649618
Produced by FastQC (version 0.11.5)