##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511775_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 34297 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.058110038778903 31.0 28.0 31.0 18.0 34.0 2 28.24427792518296 31.0 28.0 31.0 20.0 34.0 3 28.265066915473657 31.0 28.0 31.0 20.0 34.0 4 31.661136542554743 35.0 32.0 37.0 22.0 37.0 5 31.072397002653293 35.0 31.0 37.0 16.0 37.0 6 31.101933113683412 35.0 32.0 37.0 17.0 37.0 7 30.76461498090212 35.0 31.0 37.0 11.0 37.0 8 30.573169664985276 35.0 30.0 37.0 10.0 37.0 9 31.6197918185264 37.0 31.0 39.0 10.0 39.0 10 31.232585940461266 35.0 30.0 39.0 10.0 39.0 11 31.30317520482841 37.0 30.0 39.0 8.0 39.0 12 30.931101845642477 35.0 30.0 39.0 7.0 39.0 13 30.947429804356066 35.0 30.0 39.0 2.0 39.0 14 31.29804356066128 36.0 30.0 40.0 2.0 41.0 15 31.264775344782343 36.0 30.0 40.0 2.0 41.0 16 31.05869318016153 36.0 29.0 39.0 2.0 41.0 17 31.048867247864244 36.0 30.0 39.0 2.0 41.0 18 30.89582179199347 36.0 29.0 39.0 2.0 41.0 19 30.919963845234278 36.0 29.0 40.0 2.0 41.0 20 30.71650581683529 36.0 29.0 40.0 2.0 41.0 21 30.560078140945272 36.0 29.0 39.0 2.0 41.0 22 30.53360352217395 36.0 29.0 39.0 2.0 41.0 23 30.325626148059598 36.0 29.0 39.0 2.0 41.0 24 30.110417820800652 36.0 28.0 39.0 2.0 41.0 25 29.514913840860718 36.0 27.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.5209691803839718 1101 2 0.4358578977507257 1101 3 0.7173086448952297 1101 4 0.14207271807509514 1101 5 0.21191459898275866 1101 6 1.547153795071349 1101 7 -0.142328262086739 1101 8 -0.2303372730459614 1101 9 -0.6423166540898464 1101 10 -0.2901397274925195 1101 11 1.0395731849879688 1101 12 1.1142332686874745 1101 13 1.2596279159626143 1101 14 1.2968048507326024 1101 15 0.7530202237737704 1101 16 2.0184912033078355 1101 17 0.7025082364646984 1101 18 -0.15057872458700672 1101 19 -0.7310235264377525 1101 20 -0.39038305809451046 1101 21 0.5107434279090874 1101 22 -1.673724805330302 1101 23 -0.30672506570395086 1101 24 -1.1817302183094682 1101 25 -0.8886473852177552 1102 1 0.7030524120586712 1102 2 0.21453536063871326 1102 3 0.5857296975268085 1102 4 0.7938945804232702 1102 5 0.456853870237822 1102 6 0.5383818652467909 1102 7 0.08574191335185688 1102 8 0.41271265947765556 1102 9 0.09385068734065172 1102 10 -0.5013408071146515 1102 11 -1.1075253305315975 1102 12 -0.012622331852337965 1102 13 -0.6803181029307765 1102 14 -1.9447606013591638 1102 15 -0.5020405049711663 1102 16 0.5792199482471077 1102 17 0.46431660353622206 1102 18 0.29678969646562336 1102 19 1.1718104816594135 1102 20 0.19733624015110607 1102 21 -1.434600701645568 1102 22 -0.7540217014166721 1102 23 -1.3060503018712915 1102 24 -0.15608919266844268 1102 25 -0.15720339061586586 1103 1 -2.0337896932044863 1103 2 -1.7372190253261977 1103 3 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2.877760348513899 1104 16 3.143231328047964 1104 17 2.402438936395395 1104 18 2.7683401943319126 1104 19 3.2315544361402146 1104 20 4.195202527491077 1104 21 4.163550080715737 1104 22 3.512692381086886 1104 23 3.2352014762225885 1104 24 3.8439108073315573 1104 25 5.0462105496401755 1105 1 -1.0337896932044863 1105 2 0.05199666094831201 1105 3 -0.04739723745771229 1105 4 -0.4506874629203814 1105 5 -1.066365944003664 1105 6 0.0863694813458622 1105 7 0.19100507124659316 1105 8 0.6572947028213108 1105 9 1.7950890774335342 1105 10 1.353148356972035 1105 11 0.599905010025676 1105 12 1.287687265670879 1105 13 0.33051781038191663 1105 14 1.5645273243374298 1105 15 2.180622033728522 1105 16 0.3284459544390579 1105 17 0.577319699511456 1105 18 0.5258918636482868 1105 19 0.4024606364581764 1105 20 -0.8119516855454911 1105 21 -0.3014737666610472 1105 22 -0.2695015775776639 1105 23 1.3146927321844366 1105 24 2.0497931602727313 1105 25 1.5811867022633912 1106 1 0.17673662258498624 1106 2 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14 1.6527626184550819 1107 15 -0.8487897309773587 1107 16 1.0343283073802318 1107 17 1.1655549936291045 1107 18 0.5847153930600513 1107 19 1.6671665188111149 1107 20 0.4527541968074509 1107 21 -1.4485325901904602 1107 22 0.5834395988929266 1107 23 -0.5088366795802699 1107 24 1.0792049249786153 1107 25 0.9047161140280977 1108 1 -1.1900396932044863 1108 2 -0.2788856919928655 1108 3 -0.8764413551047703 1108 4 -1.408408051155675 1108 5 -1.627027708709548 1108 6 0.2554871284046847 1108 7 -0.43399492875340684 1108 8 -0.07248470894339576 1108 9 0.16457437155117915 1108 10 1.298001298148506 1108 11 -0.026933225268439998 1108 12 0.5597460892002921 1108 13 0.7275766339113297 1108 14 0.8200420302197848 1108 15 1.0078279160814638 1108 16 -0.7579511043844711 1108 17 -0.7149597122532505 1108 18 0.09942127541299328 1108 19 -0.4762158341300591 1108 20 0.7137836085721574 1108 21 2.058820350986011 1108 22 -0.3724427540482509 1108 23 -0.9573660913449764 1108 24 -1.8435891926684427 1108 25 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-1.5033525693961067 2314 18 -1.3791501531584345 2314 19 -2.4387158341300577 2314 20 -0.9808592485706988 2314 21 -0.10903679187113369 2314 22 -0.6795856111911078 2314 23 0.6676339086550236 2314 24 1.4439108073315587 2314 25 0.43076653419616306 2315 1 -2.205218264633057 2315 2 -2.43959997770715 2315 3 -2.2826913551047703 2315 4 -2.5985866225842464 2315 5 -2.2386348515666903 2315 6 -3.5480843001667424 2315 7 -3.2089949287534054 2315 8 -2.323377566086254 2315 9 -3.44524705702025 2315 10 -2.9787844161372092 2315 11 -2.942111796697013 2315 12 -3.6081110536568524 2315 13 -3.0795662232315273 2315 14 -3.952279398351642 2315 15 -1.4975292267756792 2315 16 -3.403486818670185 2315 17 -1.8176382836818235 2315 18 -1.979150153158436 2315 19 -2.0672872627014875 2315 20 -2.752287819999271 2315 21 -2.3376082204425614 2315 22 -3.0224427540482495 2315 23 -3.732366091344975 2315 24 -2.984660621239872 2315 25 -2.7406620372324078 2316 1 -7.576646836061631 2316 2 -8.296742834850008 2316 3 -5.882691355104772 2316 4 -6.570015194012818 2316 5 -6.4957777087095465 2316 6 -7.605227157309599 2316 7 -7.180423500181977 2316 8 -9.40909185180054 2316 9 -9.473818485591678 2316 10 -10.693070130422921 2316 11 -9.74211179669701 2316 12 -9.350968196513993 2316 13 -8.993851937517242 2316 14 -8.637993684065929 2316 15 -9.154672083918538 2316 16 -9.003486818670186 2316 17 -8.760495426538967 2316 18 -8.064864438872721 2316 19 -8.153001548415773 2316 20 -8.266573534284987 2316 21 -10.051893934728273 2316 22 -9.165299896905395 2316 23 -9.360937519916405 2316 24 -8.756089192668444 2316 25 -8.140662037232406 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1929.0 3 0.0 4 23.0 5 242.0 6 278.0 7 243.0 8 191.0 9 174.0 10 140.0 11 133.0 12 130.0 13 120.0 14 132.0 15 132.0 16 139.0 17 125.0 18 136.0 19 148.0 20 212.0 21 261.0 22 297.0 23 365.0 24 476.0 25 515.0 26 637.0 27 626.0 28 714.0 29 907.0 30 1119.0 31 1390.0 32 1665.0 33 2035.0 34 2615.0 35 3335.0 36 4038.0 37 4920.0 38 3755.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.59669155321329 21.540038061777192 13.462157809983896 20.401112575025618 2 18.745079744584075 23.468524943872644 39.14919672274543 18.637198588797855 3 24.529842260256 24.929294107356327 29.42531416741989 21.11554946496778 4 16.292970230632417 19.18535148846838 36.66209872583608 27.859579555063124 5 12.529895584203466 40.78632678061016 35.075541037158025 11.60823659802835 6 32.291454063037584 38.91010875586786 16.4125142140712 12.385922967023355 7 28.955885354404177 33.39067556929177 20.54698661690527 17.10645245939878 8 25.900224509432313 40.69743709362335 18.698428433973817 14.70390996297052 9 27.938303641717933 14.957576464413796 18.946263521590808 38.15785637227746 10 16.03347231536286 31.084351400997175 33.49272531125172 19.389450972388257 11 36.61544741522582 21.72493220981427 24.03125637810887 17.628363996851036 12 24.835991486135814 25.32874595445666 32.51013208152316 17.32513047788436 13 32.650086013353935 22.101058401609468 24.990523952532293 20.2583316325043 14 21.19135784470945 24.395719742251508 26.737032393503807 27.675890019535238 15 23.73385427296848 34.52196985159052 22.34014636848704 19.40402950695396 16 20.78899029069598 30.031781205353237 30.104673878181764 19.074554625769018 17 20.937691343266174 30.635332536373443 28.68180890456891 19.74516721579147 18 21.00475260226842 27.18605125812753 33.2361431028953 18.57305303670875 19 23.287751115257894 27.01985596407849 29.600256582208356 20.09213633845526 20 23.33731813278129 28.031606262938453 30.6324168294603 17.998658774819955 21 21.815319124121643 27.159809895909266 29.15706913141091 21.867801848558184 22 23.069073096772314 29.15998483832405 29.8160188937808 17.95492317112284 23 21.342974604192786 29.31451730472053 30.559524156631774 18.782983934454908 24 23.121555821208855 26.556258564889056 31.711228387322503 18.610957226579583 25 21.284660465929964 28.80426859492084 31.527538851794617 18.383532087354578 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5.0 1 5.0 2 12.0 3 19.0 4 19.0 5 19.0 6 43.0 7 67.0 8 67.0 9 67.0 10 103.5 11 140.0 12 140.0 13 140.0 14 162.5 15 185.0 16 185.0 17 185.0 18 318.0 19 451.0 20 451.0 21 451.0 22 749.5 23 1048.0 24 1048.0 25 1048.0 26 1492.0 27 1936.0 28 1936.0 29 1936.0 30 2603.0 31 3270.0 32 3270.0 33 3270.0 34 3593.0 35 3916.0 36 3916.0 37 3916.0 38 3959.0 39 4002.0 40 4002.0 41 4002.0 42 4143.0 43 4284.0 44 4284.0 45 4284.0 46 4289.0 47 4294.0 48 4294.0 49 4294.0 50 4032.0 51 3770.0 52 3770.0 53 3770.0 54 3237.0 55 2704.0 56 2704.0 57 2704.0 58 2279.5 59 1855.0 60 1855.0 61 1855.0 62 1519.0 63 1183.0 64 1183.0 65 1183.0 66 936.0 67 689.0 68 689.0 69 689.0 70 490.0 71 291.0 72 291.0 73 291.0 74 210.0 75 129.0 76 129.0 77 129.0 78 82.5 79 36.0 80 36.0 81 36.0 82 28.0 83 20.0 84 20.0 85 20.0 86 11.5 87 3.0 88 3.0 89 3.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.41403038166603495 2 0.0 3 0.0 4 0.0 5 0.032072776044552 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 34297.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.32807534186664 #Duplication Level Percentage of deduplicated Percentage of total 1 94.70645183597262 79.86412805784762 2 3.7583846207039624 6.3387468291687314 3 0.577415116520296 1.4607691634836866 4 0.26277574164995504 0.8863749015948916 5 0.15213332411313185 0.64145552089104 6 0.08298181315261738 0.4198617954923171 7 0.08298181315261738 0.48983876140770327 8 0.03803333102828296 0.256582208356416 9 0.03457575548025724 0.2624136221826982 >10 0.26277574164995504 4.411464559582471 >50 0.01728787774012862 1.087558678601627 >100 0.02420302883618007 3.8808059013907923 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 307 0.8951220223343149 No Hit GGTATCAACGCAGAGTACTTTTTTT 277 0.8076508149400823 No Hit TATCAACGCAGAGTACTTTTTTTTT 187 0.545237192757384 No Hit CTGTAGGACGTGGAATATGGCAAGA 169 0.49275446832084435 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 148 0.4315246231448815 No Hit GTCCTACAGTGGACATTTCTAAATT 133 0.3877890194477651 No Hit GTCCTAAAGTGTGTATTTCTCATTT 110 0.32072776044552 No Hit CTGTAGGACCTGGAATATGGCGAGA 100 0.2915706913141091 No Hit GTCCTACAGTGTGCATTTCTCATTT 89 0.25949791526955707 No Hit CTTTAGGACGTGAAATATGGCGAGG 67 0.1953523631804531 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 66 0.19243665626731202 No Hit GTGGTATCAACGCAGAGTACTTTTT 51 0.14870105257019564 No Hit GAATATGGCAAGAAAACTGAAAATC 50 0.14578534565705456 No Hit CTGAAGGACCTGGAATATGGCGAGA 46 0.13412251800449018 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 46 0.13412251800449018 No Hit ACAGTGGACATTTCTAAATTTTCCA 44 0.128291104178208 No Hit GTGGTATCAACGCAGAGTACATGGG 42 0.12245969035192582 No Hit GTGTATATCAATGAGTTACAATGAA 40 0.11662827652564364 No Hit GGAATATGGCGAGAAAACTGAAAAT 38 0.11079686269936145 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 35 0.1020497419599382 No Hit GATATACACTGTTCTACAAATCCCG 35 0.1020497419599382 No Hit ACCTGGAATATGGCGAGAAAACTGA 35 0.1020497419599382 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTAGGA 55 1.8748458E-4 13.817121 2 CTGTAGG 55 0.002933724 12.1076565 1 GTAGGAC 60 0.0056825983 11.082481 3 GTATCAA 100 2.1825472E-5 10.464475 1 TAGGACG 90 0.0010569171 9.49927 4 TATCAAC 110 6.331089E-5 9.49927 2 ATCAACG 110 6.331089E-5 9.49927 3 TCAACGC 115 1.0292436E-4 9.086258 4 AGGACGT 95 0.0017318333 8.999308 5 CAACGCA 120 1.6356938E-4 8.707664 5 TGGAATA 100 0.0027601453 8.549343 11 GGAATAT 100 0.0027601453 8.549343 12 ATATGGC 145 1.5230169E-5 8.516586 15 AACGCAG 125 2.5458663E-4 8.359358 6 TTTCTCA 120 0.0015901702 7.916058 16 TTCTCAT 120 0.0015901702 7.916058 17 ATTTCTC 110 0.006519383 7.7721305 15 CTCATTT 125 0.0023767757 7.5994163 19 AATATGG 160 4.4631714E-4 7.124452 14 TCTCATT 135 0.0050434335 7.036496 18 >>END_MODULE