FastQCFastQC Report
Thu 26 May 2016
SRR1511774_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511774_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804743
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33820.4202583930521918No Hit
GTCCTACAGTGGACATTTCTAAATT27860.3461974816804868No Hit
CTGTAGGACGTGGAATATGGCAAGA25810.320723510487199No Hit
GGTATCAACGCAGAGTACTTTTTTT21870.271763780486441No Hit
TATCAACGCAGAGTACTTTTTTTTT18990.2359759575417245No Hit
GTCCTAAAGTGTGTATTTCTCATTT18800.23361495533356613No Hit
GTCCTACAGTGTGCATTTCTCATTT15310.19024707266792007No Hit
CTTTAGGACGTGAAATATGGCGAGG14490.18005748419060497No Hit
CTGTAGGACCTGGAATATGGCGAGA11010.13681386479907248No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10660.13246465020509654No Hit
CTGAAGGACCTGGAATATGGCGAGA8100.10065325203201518No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG250.004594470419.9146141
TAAGACG250.0062209818.8974764
GGTCTAT400.003860701314.9359621
AAGGCGT653.5616558E-614.5365226
AGCGTGT400.005468966414.1731088
GTGCGCC400.005468966414.17310811
GGCGTGC552.0502537E-413.7436218
CTACCCT707.668863E-613.49819854
GCCCTAA450.0084290213.2764091
CGGTAGG450.0084290213.2764091
GGCCTAC450.0084290213.2764091
AGCACCG500.001560794913.2282345
TCCAACG1154.0927262E-1013.14770518
GACATTG655.729648E-513.082877
TAGGACC4900.012.534044
TAGGACT1302.1645974E-1012.3560444
GGTATCA5600.012.2688261
ATAGGAC1405.820766E-1112.1483783
AGGCGTG701.1460739E-412.1483787
CGCCACC550.00319300612.02566814