FastQCFastQC Report
Thu 26 May 2016
SRR1511774_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511774_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804743
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50480.6272810077254477No Hit
GGTATCAACGCAGAGTACTTTTTTT38740.48139592391608255No Hit
TATCAACGCAGAGTACTTTTTTTTT33920.4215010257933278No Hit
CTGTAGGACGTGGAATATGGCAAGA23480.2917701676187304No Hit
GTCCTACAGTGGACATTTCTAAATT21730.27002409464885063No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18630.23150247967363494No Hit
GTCCTAAAGTGTGTATTTCTCATTT15570.19347791779487364No Hit
CTTTAGGACGTGAAATATGGCGAGG15280.18987428284557928No Hit
CTGTAGGACCTGGAATATGGCGAGA12980.1612937297994515No Hit
GTCCTACAGTGTGCATTTCTCATTT12860.1598025705100883No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11750.14600934708347882No Hit
CTGAAGGACCTGGAATATGGCGAGA10050.12488459048416699No Hit
GTACTTTTTTTTTTTTTTTTTTTTT9750.12115669226075902No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCAGCG250.00603861218.995097
GGGACTC602.5609532E-514.2525195
CTTACAC707.238912E-613.5738263
TAGGACC4350.013.5426234
TCCAACG655.456153E-513.15126518
TGGTATC2700.012.6689052
TTGGGCT604.082125E-412.6689054
GGTATCA8000.012.3845151
GTGGTAT3000.012.0669631
GTAGAAC2400.011.8770993
ACTGTTC2750.011.742428
GGACCTG7550.011.3215786
AGGACCT8150.011.3087465
TGTAGGA13850.011.1824812
ACAGTCC855.3292417E-511.1735838
GTAGGAC13350.011.10308553
TTATTCC700.001487853710.859064
AGTACCA700.001487853710.859065
GGACCTA700.001493264210.8543386
CTGTAGG13850.010.7302671
Produced by FastQC (version 0.11.5)