##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511774_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 804743 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.85926314363716 31.0 29.0 33.0 24.0 34.0 2 29.057315192552156 31.0 29.0 33.0 24.0 34.0 3 29.086964409755662 31.0 28.0 34.0 24.0 34.0 4 32.60850979753785 35.0 32.0 37.0 26.0 37.0 5 32.110375610598666 35.0 32.0 37.0 24.0 37.0 6 32.08492773469294 35.0 32.0 37.0 23.0 37.0 7 31.838713725996996 35.0 32.0 37.0 19.0 37.0 8 31.695533853665083 35.0 32.0 37.0 19.0 37.0 9 32.84447705665038 37.0 32.0 39.0 18.0 39.0 10 32.5128519291252 37.0 32.0 39.0 17.0 39.0 11 32.58223184296104 37.0 32.0 39.0 17.0 39.0 12 32.2605552331614 37.0 32.0 39.0 16.0 39.0 13 32.27269202714407 37.0 32.0 39.0 15.0 39.0 14 32.831856878531404 38.0 32.0 40.0 11.0 41.0 15 32.77004584072182 38.0 32.0 40.0 10.0 41.0 16 32.58962799303629 37.0 32.0 40.0 10.0 41.0 17 32.53172006466661 37.0 32.0 40.0 10.0 41.0 18 32.39060296268498 37.0 31.0 40.0 10.0 41.0 19 32.440863729165706 37.0 31.0 40.0 10.0 41.0 20 32.24211580591568 37.0 31.0 40.0 2.0 41.0 21 32.06875486956705 37.0 31.0 40.0 2.0 41.0 22 32.13013595644821 37.0 31.0 40.0 2.0 41.0 23 31.94341174760141 37.0 31.0 40.0 2.0 41.0 24 31.705007685683505 37.0 31.0 40.0 2.0 41.0 25 31.137671281390457 37.0 30.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7093034921659545 1101 2 0.5503125885839175 1101 3 0.5450428370555578 1101 4 0.7923438740220803 1101 5 0.8908981459706382 1101 6 0.6895060981560839 1101 7 0.6732152041421919 1101 8 0.7702357346230002 1101 9 0.9426535424520779 1101 10 1.059461477517047 1101 11 0.3823840046989915 1101 12 0.4598584416973779 1101 13 0.6208212696057274 1101 14 0.5876604987878977 1101 15 0.01207594005611412 1101 16 0.41743373838431097 1101 17 0.628898535778319 1101 18 0.6046866648694618 1101 19 0.6028041859503475 1101 20 0.3440027751550332 1101 21 0.6865426707523419 1101 22 0.07697158840547047 1101 23 0.09806491602439849 1101 24 -0.11232287816535447 1101 25 -0.25500340140089506 1102 1 0.5602752580233172 1102 2 0.8566959816746937 1102 3 0.9716435541448547 1102 4 0.937020135170421 1102 5 0.9026681980045552 1102 6 0.9362727045659405 1102 7 1.347541309987765 1102 8 0.992610818266769 1102 9 1.0672378204190593 1102 10 1.0907617262012153 1102 11 1.3108798150198808 1102 12 1.166863045850107 1102 13 1.3043307126131083 1102 14 1.3813575035917935 1102 15 1.4122202567738782 1102 16 1.2967445136996645 1102 17 1.4443254436053792 1102 18 1.5006862454021856 1102 19 1.7818318208540234 1102 20 1.4840848708934402 1102 21 1.2267241402860343 1102 22 1.2524437387745024 1102 23 1.475463120304962 1102 24 1.1521762079952182 1102 25 1.4253001131356413 1103 1 0.39553605575756023 1103 2 0.44429727189575985 1103 3 0.49215728241171064 1103 4 0.522841471021259 1103 5 0.49415391903357886 1103 6 0.482703945223637 1103 7 0.6734008017665367 1103 8 0.4922715451962034 1103 9 0.7051154460976079 1103 10 0.6769911731369405 1103 11 0.7506121334842035 1103 12 0.5568844839745637 1103 13 0.3094788126839134 1103 14 0.5014601705731536 1103 15 0.7147436616987477 1103 16 0.38731075553744176 1103 17 0.7595543791729966 1103 18 0.7678367449694932 1103 19 0.8656519372886038 1103 20 0.6840347174830086 1103 21 0.3949150772697472 1103 22 0.8037083914375458 1103 23 0.9663125814016489 1103 24 1.0775410494711082 1103 25 0.5254004199565045 1104 1 0.41555893905275454 1104 2 0.6010918503150542 1104 3 0.5100238551981917 1104 4 0.6199267846397518 1104 5 0.7503368093697915 1104 6 0.8207056174644443 1104 7 0.7706694751221654 1104 8 0.6937508915554105 1104 9 0.758359446567006 1104 10 0.665355164218326 1104 11 0.6988496796908592 1104 12 0.7994437458416073 1104 13 0.7554720357080313 1104 14 0.9782174902938578 1104 15 0.9871648903556718 1104 16 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-0.8857960547763604 2315 7 -0.9779325315667933 2315 8 -1.0868600337511651 2315 9 -1.1335424486392363 2315 10 -0.9471754935297234 2315 11 -1.0779931296736898 2315 12 -0.7188260423412274 2315 13 -0.8609159241581885 2315 14 -1.0552942153917613 2315 15 -1.1173791453187967 2315 16 -1.2907681918309812 2315 17 -1.0925684278445509 2315 18 -1.0832509743287524 2315 19 -1.0206375363956042 2315 20 -0.7562021246222521 2315 21 -1.1417691332565667 2315 22 -0.8738880997905234 2315 23 -1.0751347870194046 2315 24 -0.7051870207043294 2315 25 -0.8374592291663028 2316 1 -1.9602345691518188 2316 2 -1.6309165892032667 2316 3 -1.4010571586496212 2316 4 -1.6018685478275145 2316 5 -1.2473460084711299 2316 6 -1.435856660836965 2316 7 -1.728114349748612 2316 8 -1.479609345045933 2316 9 -1.5361760574546608 2316 10 -1.6751093778272441 2316 11 -1.9322300442742417 2316 12 -1.339619430770977 2316 13 -1.5162878249846372 2316 14 -1.8108314054744064 2316 15 -1.9581394758973083 2316 16 -1.8673742524370418 2316 17 -1.9450091991944127 2316 18 -1.9022372002240715 2316 19 -1.719249106643538 2316 20 -1.8685877995533815 2316 21 -2.179664450060976 2316 22 -1.9561690915260606 2316 23 -2.007090709884416 2316 24 -2.0667517589963396 2316 25 -1.7391892567145106 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33874.0 3 2.0 4 532.0 5 4123.0 6 5061.0 7 4161.0 8 3464.0 9 3015.0 10 2797.0 11 2683.0 12 2562.0 13 2348.0 14 2348.0 15 2356.0 16 2372.0 17 2463.0 18 2653.0 19 3024.0 20 3856.0 21 4665.0 22 5968.0 23 7170.0 24 9013.0 25 10094.0 26 11790.0 27 13018.0 28 15423.0 29 18384.0 30 22815.0 31 28756.0 32 36236.0 33 46335.0 34 58900.0 35 76843.0 36 102838.0 37 135430.0 38 117371.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.99634392083317 23.043648497665696 14.717963169865527 21.242044411635607 2 16.57199876233779 24.041712695854454 40.20513381290673 19.181154728901028 3 21.624170697974385 26.737604427748984 30.736023798902256 20.902201075374375 4 14.828833553072224 19.93605411914114 38.31931436495875 26.915797962827888 5 12.232595312346573 39.970218970118296 36.4508122042561 11.346373513279033 6 30.13247707653251 40.41899090765623 17.625627063546993 11.822904952264263 7 26.764196768409292 33.175436133026324 22.79100284190108 17.26936425666331 8 23.90564441070006 40.705790544310425 20.414343461204385 14.974221583785132 9 26.310387291346434 15.494263385950546 21.18316033814522 37.0121889845578 10 14.415906693192735 31.585984593839278 35.318480558389446 18.67962815457854 11 34.31120245842462 22.936639399162218 25.3981705960785 17.35398754633467 12 22.893892832867138 26.43328366944478 33.16686196711248 17.5059615305756 13 29.88482037122411 23.04934618878325 26.435520408378828 20.63031303161382 14 19.681562933756492 24.698941152641275 28.959058979077795 26.66043693452444 15 22.191805334125306 34.17078495867625 24.032020160473593 19.605389546724854 16 20.197131258053812 30.62455964202236 30.125766859730373 19.052542240193453 17 20.105673488306206 31.09999092878099 29.298173454133803 19.496162128779 18 19.612496924480144 28.931632746892795 32.98485725859965 18.47101307002741 19 22.65530734656903 28.011302987413373 29.71818332063777 19.61520634537983 20 22.378324508569815 28.86263067836564 30.798031172684944 17.9610136403796 21 21.489842098657583 28.019380100230755 29.484568365304202 21.006209435807456 22 21.48549288406361 30.07767697264841 30.1205478022176 18.316282341070377 23 20.62521823737516 29.681649918048368 30.5911328212858 19.101999023290666 24 21.898153095944068 28.224253073823967 31.47690189362966 18.400691936602303 25 21.200583541060364 29.33909252903415 30.934759214754543 18.525564715150942 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 140.0 1 140.0 2 274.0 3 408.0 4 408.0 5 408.0 6 1096.0 7 1784.0 8 1784.0 9 1784.0 10 2752.0 11 3720.0 12 3720.0 13 3720.0 14 5048.0 15 6376.0 16 6376.0 17 6376.0 18 10560.0 19 14744.0 20 14744.0 21 14744.0 22 22789.0 23 30834.0 24 30834.0 25 30834.0 26 42976.0 27 55118.0 28 55118.0 29 55118.0 30 69257.0 31 83396.0 32 83396.0 33 83396.0 34 90357.0 35 97318.0 36 97318.0 37 97318.0 38 99451.0 39 101584.0 40 101584.0 41 101584.0 42 102260.0 43 102936.0 44 102936.0 45 102936.0 46 100351.0 47 97766.0 48 97766.0 49 97766.0 50 89645.5 51 81525.0 52 81525.0 53 81525.0 54 68445.5 55 55366.0 56 55366.0 57 55366.0 58 45155.5 59 34945.0 60 34945.0 61 34945.0 62 27490.0 63 20035.0 64 20035.0 65 20035.0 66 15026.5 67 10018.0 68 10018.0 69 10018.0 70 7197.0 71 4376.0 72 4376.0 73 4376.0 74 3014.0 75 1652.0 76 1652.0 77 1652.0 78 1084.5 79 517.0 80 517.0 81 517.0 82 324.0 83 131.0 84 131.0 85 131.0 86 87.5 87 44.0 88 44.0 89 44.0 90 25.5 91 7.0 92 7.0 93 7.0 94 4.5 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3468187980510548 2 0.0 3 0.0 4 0.0 5 0.025349707919174198 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.2426327411359898E-4 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005591847335111954 25 1.2426327411359898E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 804743.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.70280201500331 #Duplication Level Percentage of deduplicated Percentage of total 1 92.39072798518563 73.63799900625277 2 5.638018197921137 8.987316963717884 3 0.9363086836311855 2.2387927690915426 4 0.33941826029635686 1.0821034560270957 5 0.17220350346530516 0.6862550871492578 6 0.10604872761391089 0.5071428444572733 7 0.07272390421209883 0.4057409257422485 8 0.04961798012908866 0.31637536372904984 9 0.039761845403638856 0.2852217442761657 >10 0.21105829436901005 3.111055385662615 >50 0.022057404798508408 1.2133942510262692 >100 0.017240342579328587 2.7459789318472234 >500 0.0029510495968135646 1.4962874016883183 >1k 0.0017085023981552217 2.6614282161741674 >5k 1.553183998322929E-4 0.6249076531581559 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5048 0.6272810077254477 No Hit GGTATCAACGCAGAGTACTTTTTTT 3874 0.48139592391608255 No Hit TATCAACGCAGAGTACTTTTTTTTT 3392 0.4215010257933278 No Hit CTGTAGGACGTGGAATATGGCAAGA 2348 0.2917701676187304 No Hit GTCCTACAGTGGACATTTCTAAATT 2173 0.27002409464885063 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1863 0.23150247967363494 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1557 0.19347791779487364 No Hit CTTTAGGACGTGAAATATGGCGAGG 1528 0.18987428284557928 No Hit CTGTAGGACCTGGAATATGGCGAGA 1298 0.1612937297994515 No Hit GTCCTACAGTGTGCATTTCTCATTT 1286 0.1598025705100883 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1175 0.14600934708347882 No Hit CTGAAGGACCTGGAATATGGCGAGA 1005 0.12488459048416699 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 975 0.12115669226075902 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 1.24263274113599E-4 0.0 0.0 0.0 11 0.0 1.24263274113599E-4 0.0 0.0 0.0 12 0.0 1.24263274113599E-4 0.0 0.0 0.0 13 0.0 1.24263274113599E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCAGCG 25 0.006038612 18.99509 7 GGGACTC 60 2.5609532E-5 14.252519 5 CTTACAC 70 7.238912E-6 13.573826 3 TAGGACC 435 0.0 13.542623 4 TCCAACG 65 5.456153E-5 13.151265 18 TGGTATC 270 0.0 12.668905 2 TTGGGCT 60 4.082125E-4 12.668905 4 GGTATCA 800 0.0 12.384515 1 GTGGTAT 300 0.0 12.066963 1 GTAGAAC 240 0.0 11.877099 3 ACTGTTC 275 0.0 11.74242 8 GGACCTG 755 0.0 11.321578 6 AGGACCT 815 0.0 11.308746 5 TGTAGGA 1385 0.0 11.182481 2 ACAGTCC 85 5.3292417E-5 11.173583 8 GTAGGAC 1335 0.0 11.1030855 3 TTATTCC 70 0.0014878537 10.85906 4 AGTACCA 70 0.0014878537 10.85906 5 GGACCTA 70 0.0014932642 10.854338 6 CTGTAGG 1385 0.0 10.730267 1 >>END_MODULE