FastQCFastQC Report
Thu 26 May 2016
SRR1511773_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511773_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1347645
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT48780.3619647607493071No Hit
GTCCTAAAGTGTGTATTTCTCATTT47990.3561026828281929No Hit
CTGTAGGACGTGGAATATGGCAAGA45860.3402973334965811No Hit
GTATCAACGCAGAGTACTTTTTTTT45290.33606773297122017No Hit
CTTTAGGACGTGAAATATGGCGAGG40130.29777871768900566No Hit
GGTATCAACGCAGAGTACTTTTTTT29110.21600644086536142No Hit
TATCAACGCAGAGTACTTTTTTTTT27650.2051727272389984No Hit
GTCCTACAGTGTGCATTTCTCATTT22450.16658689788482872No Hit
CTGAAGGACCTGGAATATGGCGAGA21270.15783088276215176No Hit
ATTTAGAAATGTCCACTGTAGGACG19010.1410608876966857No Hit
TTTCTAAATTTTCCACCTTTTTCAG18650.13838956104908934No Hit
GTCCTTCAGTGTGCATTTCTCATTT17580.13044978462428905No Hit
CTGTAGGACCTGGAATATGGCGAGA17410.12918832481847964No Hit
GAATATGGCAAGAAAACTGAAAATC15740.11679633731435209No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG401.9011287E-417.4572161
CGGTAGG702.5484042E-715.6758681
GGTATCA7550.014.0054121
TAGGACC7100.012.6403754
GCGCCAC803.034447E-511.80877213
AAATGTC7450.011.792927
AATGTCC7250.011.7273328
GGTCTAT600.00408144811.63814451
GAAATGT7700.011.4100346
TAGAAAT8000.011.336424
TCCAACG1251.9879735E-811.3364218
CCAACGA855.6332654E-511.11413719
AAGACGT600.006124635711.0215195
AAGGCCG600.006124635711.0215195
ACCTTTT7150.010.96646715
ATGTCCA7950.010.9323979
AAGGCGT1053.7045102E-610.7965916
CGTTTCC700.001562380310.79659114
GTGCGCC700.001562380310.79659111
GGCGAGG12000.010.78534419
Produced by FastQC (version 0.11.5)