##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511773_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1347645 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.544961766637357 31.0 31.0 34.0 27.0 34.0 2 27.775412664314416 31.0 28.0 34.0 10.0 34.0 3 29.548611837687226 31.0 30.0 34.0 16.0 34.0 4 34.00869813637865 37.0 35.0 37.0 30.0 37.0 5 34.38963376853697 37.0 35.0 37.0 32.0 37.0 6 34.612740744038675 37.0 35.0 37.0 32.0 37.0 7 34.63488084770099 37.0 35.0 37.0 32.0 37.0 8 34.65981916602666 37.0 35.0 37.0 32.0 37.0 9 36.333201251071316 39.0 37.0 39.0 32.0 39.0 10 36.22480104181739 39.0 37.0 39.0 32.0 39.0 11 36.23648141758401 39.0 37.0 39.0 32.0 39.0 12 36.15367400168442 39.0 37.0 39.0 32.0 39.0 13 36.151782554010886 39.0 37.0 39.0 32.0 39.0 14 37.37915919993767 40.0 38.0 41.0 32.0 41.0 15 37.348093155096485 40.0 38.0 41.0 32.0 41.0 16 37.321613629702185 40.0 38.0 41.0 32.0 41.0 17 37.294924850387154 40.0 38.0 41.0 32.0 41.0 18 37.27824983582472 40.0 38.0 41.0 32.0 41.0 19 37.313209339254776 40.0 38.0 41.0 32.0 41.0 20 37.277255508683666 40.0 38.0 41.0 32.0 41.0 21 37.187319360810896 40.0 38.0 41.0 32.0 41.0 22 37.11204434402235 40.0 38.0 41.0 32.0 41.0 23 37.02534717970979 40.0 38.0 41.0 32.0 41.0 24 36.96190762404046 40.0 38.0 41.0 31.0 41.0 25 36.88418166505274 40.0 38.0 41.0 31.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8447742054431124 1101 2 3.5127614064497408 1101 3 1.9957805437750835 1101 4 0.9496932503536399 1101 5 0.5065042205320509 1101 6 0.4231022005605425 1101 7 0.2667057206875967 1101 8 0.26164919712090295 1101 9 0.19885608791522458 1101 10 0.28082306893957565 1101 11 0.2445113318047305 1101 12 0.28493723156057627 1101 13 0.20284448461588767 1101 14 0.14661356743754794 1101 15 0.2643536799344943 1101 16 0.3095772448647196 1101 17 0.40090254629640754 1101 18 0.5168997561262785 1101 19 0.4693973110361114 1101 20 0.4700624465769323 1101 21 0.3542304050908385 1101 22 0.35811231001923716 1101 23 0.1921332932380153 1101 24 0.39847599242423115 1101 25 0.4889355553103485 1102 1 1.1686331299784705 1102 2 3.9752892524309758 1102 3 2.292898112082426 1102 4 1.4098649604627482 1102 5 1.1185732514648024 1102 6 0.8628150414197435 1102 7 0.705531284246895 1102 8 0.7191097520350738 1102 9 0.8128086183086722 1102 10 0.7931468465619744 1102 11 0.7104386910571847 1102 12 0.8809116918499029 1102 13 0.7866805361505982 1102 14 0.9757013032814896 1102 15 0.97467634100758 1102 16 1.1363570841032953 1102 17 1.0666689273215724 1102 18 0.9940901711465742 1102 19 1.037902489982109 1102 20 0.998938254855652 1102 21 1.0690675027130183 1102 22 1.1448340453320114 1102 23 1.3016049919648296 1102 24 0.9970640453464981 1102 25 1.053413026488741 1103 1 0.9040952154708997 1103 2 3.5924198978516166 1103 3 2.062157250311266 1103 4 1.285016032360005 1103 5 0.7104509000858314 1103 6 0.5367907350537138 1103 7 0.6114844247269033 1103 8 0.4829048855748539 1103 9 0.6017073625316343 1103 10 0.6664539335845774 1103 11 0.6560372469913887 1103 12 0.6234898472425883 1103 13 0.6377363536505598 1103 14 0.8336257699263712 1103 15 0.6266346996276226 1103 16 0.6594221967393921 1103 17 0.7260203425994192 1103 18 0.8098400323938506 1103 19 0.8150458954668593 1103 20 0.7430035282893073 1103 21 0.8900847806697527 1103 22 0.8231922504163265 1103 23 0.8732496397403722 1103 24 0.8351559489302076 1103 25 0.7168844901095852 1104 1 0.9060509524281457 1104 2 3.6747901788953996 1104 3 1.987697010520911 1104 4 1.23691366408881 1104 5 0.9349767176173813 1104 6 0.7643117418330903 1104 7 0.5460025378401312 1104 8 0.6047176656330322 1104 9 0.6589416567270376 1104 10 0.7587703867692781 1104 11 0.7641902737455624 1104 12 0.6414640748037925 1104 13 0.8196399891568475 1104 14 0.8061078552212422 1104 15 0.8018254419573481 1104 16 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-7.0966199439873066 2315 4 -3.012821447843379 2315 5 -1.9272684255789159 2315 6 -1.316924256194092 2315 7 -1.0575963871427305 2315 8 -0.9872277517511492 2315 9 -0.9541347229897497 2315 10 -1.005633359071922 2315 11 -1.105340597469727 2315 12 -1.1205070894915963 2315 13 -1.106898242498481 2315 14 -1.1224554378755798 2315 15 -1.1622250153011109 2315 16 -1.166362895366973 2315 17 -0.9290137284183402 2315 18 -1.020155487319414 2315 19 -1.1882653490109263 2315 20 -0.9935050780289387 2315 21 -0.972184432466868 2315 22 -0.9552810137330496 2315 23 -0.992260487791512 2315 24 -1.2249747087342087 2315 25 -1.2298079121576464 2316 1 -1.5398159923924162 2316 2 -1.7681030038349483 2316 3 -0.8932207429600574 2316 4 -1.0134590209636514 2316 5 -1.2081260728895131 2316 6 -1.1933019491602792 2316 7 -1.2352875699077472 2316 8 -1.4488083980630293 2316 9 -1.2585736586439111 2316 10 -1.552390207409772 2316 11 -1.5569385429683678 2316 12 -1.4591030018899431 2316 13 -1.491952280164334 2316 14 -1.6576022134498416 2316 15 -2.0758539209937865 2316 16 -1.90279551770967 2316 17 -1.9515606323989303 2316 18 -1.684457634558676 2316 19 -1.7808784179223238 2316 20 -2.1067567901134012 2316 21 -2.0620019884662923 2316 22 -1.9846584213518241 2316 23 -1.85458036223865 2316 24 -1.789614814312749 2316 25 -1.663807947826072 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29693.0 3 2.0 4 255.0 5 1354.0 6 1601.0 7 1476.0 8 1380.0 9 1266.0 10 1208.0 11 1139.0 12 1157.0 13 1160.0 14 1154.0 15 1258.0 16 1227.0 17 1292.0 18 1576.0 19 1872.0 20 2386.0 21 2998.0 22 3669.0 23 4381.0 24 5187.0 25 5938.0 26 6952.0 27 8139.0 28 9526.0 29 11847.0 30 15398.0 31 20671.0 32 28369.0 33 41206.0 34 60615.0 35 94993.0 36 160757.0 37 228015.0 38 586528.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.10978781504031 20.272623725090806 13.251857870581643 25.36573058928724 2 15.070688170475908 21.29130985653941 42.410865049571434 21.227136923413248 3 17.931651139580527 25.785870908139756 30.63885518812447 25.64362276415525 4 11.491453609815641 17.606120306163714 40.23055033039116 30.67187575362948 5 11.848333495344828 38.71624564702321 37.19172984496613 12.243691012665836 6 29.5046069527625 38.736989263429244 19.260008444410477 12.498395339397776 7 26.139821688946274 32.145186603296864 24.057670974180887 17.65732073357598 8 22.914120558455675 39.462618122725196 21.632328988717354 15.99093233010177 9 25.75863821703787 15.163637308044775 21.902949218822464 37.1747752560949 10 14.51517276434076 29.222087419164545 36.27483498992687 19.987904826567828 11 32.328024071621236 22.079256777563824 27.628047445729404 17.96467170508554 12 23.343313706502826 24.606109175636018 33.80986832585733 18.24070879200383 13 28.47673421170576 23.235142218568107 26.595747838449917 21.69237573127621 14 19.08662889707601 24.101599456830247 28.962597716757756 27.84917392933599 15 20.974885819336695 34.44393738707152 23.868526206827465 20.71265058676432 16 19.61918754568154 28.6664514764645 31.27047553324503 20.443885444608927 17 19.801816361800157 30.183973601238907 29.915652485569204 20.09855755139173 18 19.525839520051647 27.4507010377362 33.80088970018068 19.22256974203147 19 22.5639636698228 26.918464871924254 29.20987800896382 21.30769344928913 20 22.5140987207266 28.185865661452613 30.246653408922263 19.05338220889852 21 20.929968530101206 27.37302404134051 28.75514697207037 22.941860456487913 22 21.809800563373592 30.154215358312136 28.873193449668893 19.16279062864538 23 19.751862515211776 29.72188418271942 30.294960078359207 20.231293223709596 24 22.061757903024375 27.230882364944936 31.17146785770735 19.535891874323344 25 20.904844412619145 28.62948795482456 30.530818804795068 19.934848827761225 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 224.0 1 224.0 2 345.5 3 467.0 4 467.0 5 467.0 6 1332.5 7 2198.0 8 2198.0 9 2198.0 10 3055.0 11 3912.0 12 3912.0 13 3912.0 14 6138.5 15 8365.0 16 8365.0 17 8365.0 18 14802.0 19 21239.0 20 21239.0 21 21239.0 22 33577.0 23 45915.0 24 45915.0 25 45915.0 26 66397.5 27 86880.0 28 86880.0 29 86880.0 30 111723.0 31 136566.0 32 136566.0 33 136566.0 34 149134.0 35 161702.0 36 161702.0 37 161702.0 38 165560.0 39 169418.0 40 169418.0 41 169418.0 42 171468.5 43 173519.0 44 173519.0 45 173519.0 46 170746.5 47 167974.0 48 167974.0 49 167974.0 50 154812.0 51 141650.0 52 141650.0 53 141650.0 54 118788.5 55 95927.0 56 95927.0 57 95927.0 58 79504.0 59 63081.0 60 63081.0 61 63081.0 62 49999.5 63 36918.0 64 36918.0 65 36918.0 66 27968.5 67 19019.0 68 19019.0 69 19019.0 70 13566.0 71 8113.0 72 8113.0 73 8113.0 74 5555.0 75 2997.0 76 2997.0 77 2997.0 78 2032.0 79 1067.0 80 1067.0 81 1067.0 82 708.5 83 350.0 84 350.0 85 350.0 86 222.5 87 95.0 88 95.0 89 95.0 90 65.0 91 35.0 92 35.0 93 35.0 94 22.0 95 9.0 96 9.0 97 9.0 98 7.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.298576405507386 3 0.0 4 0.0 5 2.9681407195515137E-4 6 5.936281439103027E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 7.420351798878784E-5 14 0.0 15 0.0 16 0.0 17 4.452211079327271E-4 18 0.0 19 3.7101758994393926E-4 20 3.7101758994393926E-4 21 5.936281439103027E-4 22 0.003635972381450605 23 3.7101758994393926E-4 24 0.0020776985036860597 25 7.420351798878785E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1347645.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.74657200041148 #Duplication Level Percentage of deduplicated Percentage of total 1 90.06699493190693 68.22266116470384 2 7.28507811156552 11.036393874126386 3 1.3285658652048824 3.019029298980917 4 0.44708050120818343 1.3545926149898286 5 0.21853133060443286 0.8276499583987198 6 0.132433358238975 0.6018823743062884 7 0.09431313836740382 0.5000727848151926 8 0.0633511090190896 0.3838903472496309 9 0.04788363366193968 0.3264318994333896 >10 0.26390630966852746 3.636035061225218 >50 0.025235264461520194 1.3236107444296108 >100 0.02169567713132892 3.4057199087748944 >500 0.00276123061833205 1.554161786578792 >1k 0.0021695383429751825 3.807868181987267 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 4878 0.3619647607493071 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4799 0.3561026828281929 No Hit CTGTAGGACGTGGAATATGGCAAGA 4586 0.3402973334965811 No Hit GTATCAACGCAGAGTACTTTTTTTT 4529 0.33606773297122017 No Hit CTTTAGGACGTGAAATATGGCGAGG 4013 0.29777871768900566 No Hit GGTATCAACGCAGAGTACTTTTTTT 2911 0.21600644086536142 No Hit TATCAACGCAGAGTACTTTTTTTTT 2765 0.2051727272389984 No Hit GTCCTACAGTGTGCATTTCTCATTT 2245 0.16658689788482872 No Hit CTGAAGGACCTGGAATATGGCGAGA 2127 0.15783088276215176 No Hit ATTTAGAAATGTCCACTGTAGGACG 1901 0.1410608876966857 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1865 0.13838956104908934 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1758 0.13044978462428905 No Hit CTGTAGGACCTGGAATATGGCGAGA 1741 0.12918832481847964 No Hit GAATATGGCAAGAAAACTGAAAATC 1574 0.11679633731435209 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.4840703597757569E-4 0.0 5 0.0 0.0 0.0 1.4840703597757569E-4 0.0 6 0.0 0.0 0.0 1.4840703597757569E-4 0.0 7 0.0 0.0 0.0 1.4840703597757569E-4 0.0 8 0.0 0.0 0.0 1.4840703597757569E-4 0.0 9 0.0 0.0 0.0 2.9681407195515137E-4 0.0 10 0.0 0.0 0.0 2.9681407195515137E-4 0.0 11 0.0 0.0 0.0 3.7101758994393926E-4 0.0 12 0.0 0.0 0.0 4.452211079327271E-4 0.0 13 0.0 0.0 0.0 4.452211079327271E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTAGG 40 1.9011287E-4 17.457216 1 CGGTAGG 70 2.5484042E-7 15.675868 1 GGTATCA 755 0.0 14.005412 1 TAGGACC 710 0.0 12.640375 4 GCGCCAC 80 3.034447E-5 11.808772 13 AAATGTC 745 0.0 11.79292 7 AATGTCC 725 0.0 11.727332 8 GGTCTAT 60 0.004081448 11.6381445 1 GAAATGT 770 0.0 11.410034 6 TAGAAAT 800 0.0 11.33642 4 TCCAACG 125 1.9879735E-8 11.33642 18 CCAACGA 85 5.6332654E-5 11.114137 19 AAGACGT 60 0.0061246357 11.021519 5 AAGGCCG 60 0.0061246357 11.021519 5 ACCTTTT 715 0.0 10.966467 15 ATGTCCA 795 0.0 10.932397 9 AAGGCGT 105 3.7045102E-6 10.796591 6 CGTTTCC 70 0.0015623803 10.796591 14 GTGCGCC 70 0.0015623803 10.796591 11 GGCGAGG 1200 0.0 10.785344 19 >>END_MODULE