FastQCFastQC Report
Thu 26 May 2016
SRR1511773_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511773_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1347645
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57670.4279316882413395No Hit
CTGTAGGACGTGGAATATGGCAAGA46590.3457141903097626No Hit
CTTTAGGACGTGAAATATGGCGAGG42170.3129162353587183No Hit
TATCAACGCAGAGTACTTTTTTTTT41740.30972548408520045No Hit
GTCCTAAAGTGTGTATTTCTCATTT41500.30794459965346954No Hit
GTCCTACAGTGGACATTTCTAAATT41000.3042344237540302No Hit
GGTATCAACGCAGAGTACTTTTTTT40670.3017857076604002No Hit
CTGAAGGACCTGGAATATGGCGAGA24620.18268906128839568No Hit
TTTCTAAATTTTCCACCTTTTTCAG20890.15501114907857783No Hit
ATTTAGAAATGTCCACTGTAGGACG19920.1478134078336654No Hit
GTCCTACAGTGTGCATTTCTCATTT19110.1418029228765736No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18930.1404672595527754No Hit
CTGTAGGACCTGGAATATGGCGAGA18040.13386314645177327No Hit
GAATATGGCAAGAAAACTGAAAATC16680.12377146800529813No Hit
GGAATATGGCGAGAAAACTGAAAAT16020.11887403581803814No Hit
GTCCTTCAGTGTGCATTTCTCATTT14530.10781771163770874No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14520.10774350811971996No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14220.10551740258005632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10250.014.0480271
ATACGGC551.964496E-413.81432315
TCGAACT751.4819232E-512.66359919
CACCTTT7700.012.4575614
ACCTTTT7700.012.08753315
TAGGACC6250.012.0082114
CCACCTT7800.011.81080313
GTACTAG657.772205E-411.7365071
ACTAGCC658.021021E-411.6925133
CCTACAC1406.7848305E-1011.5359173
TAGAAAT6600.011.5153534
GGCGAGG11750.011.47807119
TTCCACC8300.011.32816111
TGGTATC2700.011.2594582
CTCGAAC855.3345524E-511.17376418
GCCTCGA855.3365693E-511.17334916
GAATACG600.005887196411.08023813
TCCTATA1658.0035534E-1110.9395862
TCCAACG1053.4745572E-610.85451418
GTGGTAT2900.010.8512111
Produced by FastQC (version 0.11.5)