##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511773_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1347645 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.499955848906797 31.0 30.0 34.0 26.0 34.0 2 29.724514245220366 31.0 30.0 34.0 26.0 34.0 3 29.777739686638544 31.0 30.0 34.0 26.0 34.0 4 33.37502680602088 35.0 33.0 37.0 28.0 37.0 5 32.894259986865976 35.0 33.0 37.0 26.0 37.0 6 32.83366613611151 35.0 33.0 37.0 26.0 37.0 7 32.58986750961863 35.0 33.0 37.0 25.0 37.0 8 32.457607159155415 35.0 33.0 37.0 25.0 37.0 9 33.74058672721674 37.0 33.0 39.0 25.0 39.0 10 33.43477547870545 37.0 33.0 39.0 23.0 39.0 11 33.43685466127949 37.0 33.0 39.0 23.0 39.0 12 33.20422218017356 37.0 33.0 39.0 21.0 39.0 13 33.196710558047556 37.0 33.0 39.0 19.0 39.0 14 33.898304820631544 38.0 33.0 40.0 18.0 41.0 15 33.831789529141574 38.0 33.0 40.0 18.0 41.0 16 33.686648189990684 38.0 33.0 40.0 17.0 41.0 17 33.613227519116684 38.0 33.0 40.0 17.0 41.0 18 33.50359033721789 38.0 33.0 40.0 17.0 41.0 19 33.52578906165942 38.0 32.0 40.0 18.0 41.0 20 33.35182039780506 38.0 32.0 40.0 16.0 41.0 21 33.179444141446744 38.0 32.0 40.0 11.0 41.0 22 33.25923295823455 38.0 32.0 40.0 10.0 41.0 23 33.08630759584312 38.0 32.0 40.0 8.0 41.0 24 32.90229622786416 38.0 32.0 40.0 2.0 41.0 25 32.37750297741616 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4600698188173311 1101 2 0.4390603621040121 1101 3 0.6144131871129552 1101 4 0.6303068286667823 1101 5 0.7809031176043248 1101 6 0.7101221055430713 1101 7 0.6378835524648281 1101 8 0.5077661419534039 1101 9 0.6656427375874188 1101 10 0.7207552644170079 1101 11 0.5257152831619081 1101 12 0.6858718664961572 1101 13 0.7654900039922339 1101 14 0.8603304448199083 1101 15 0.7071527945226777 1101 16 0.6210351608579998 1101 17 0.662355215046901 1101 18 0.7966227252729752 1101 19 0.6974238676988236 1101 20 0.7408198803880239 1101 21 0.8182550296589213 1101 22 0.8813542246102202 1101 23 0.45266788281649184 1101 24 0.8153333856908702 1101 25 0.4982286039792001 1102 1 0.850583485706224 1102 2 0.9147699942389522 1102 3 1.0722227991933693 1102 4 1.0592167838422029 1102 5 1.0635852208363588 1102 6 1.073009848975218 1102 7 1.2481606843423094 1102 8 1.162226648086289 1102 9 1.243833168868143 1102 10 1.2754022234461502 1102 11 1.2350157053909925 1102 12 1.3931592478627906 1102 13 1.5085188879307267 1102 14 1.6421773041184338 1102 15 1.545744207933197 1102 16 1.536570372812669 1102 17 1.5937736120940116 1102 18 1.652874588446906 1102 19 1.736942125448273 1102 20 1.609244299213259 1102 21 1.543836475427689 1102 22 1.3920151025648764 1102 23 1.5198624713463857 1102 24 1.4647683358526606 1102 25 1.6894593556987445 1103 1 0.2809711115562479 1103 2 0.3884059381984102 1103 3 0.31809004265642926 1103 4 0.3046332754470171 1103 5 0.4375339419770867 1103 6 0.5548967823583482 1103 7 0.6081143124020016 1103 8 0.40249450569227463 1103 9 0.6712486334434047 1103 10 0.6926049684160418 1103 11 0.7127234584266944 1103 12 0.7050172895560465 1103 13 0.3306384071979025 1103 14 0.8801846593098617 1103 15 0.7609162353828935 1103 16 0.6514809745586163 1103 17 0.6843519127231943 1103 18 0.7568820785007944 1103 19 0.776808507866356 1103 20 0.85845509443498 1103 21 0.8545886448979374 1103 22 0.9917484898431965 1103 23 1.0418507590598978 1103 24 0.9416835125484226 1103 25 0.866502133035624 1104 1 0.3594324954140724 1104 2 0.4864947786448006 1104 3 0.5764457291097962 1104 4 0.4309584225137968 1104 5 0.6088446464804349 1104 6 0.5782960541748636 1104 7 0.6129905134795877 1104 8 0.49262331635168977 1104 9 0.596938359253727 1104 10 0.6318132428067074 1104 11 0.6110385356383645 1104 12 0.8435478843422928 1104 13 0.985636101025861 1104 14 0.8759428487973224 1104 15 0.8064578508370843 1104 16 0.9823870296130011 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-1.657575887647866 2315 16 -1.7408671124631354 2315 17 -1.712025827545208 2315 18 -1.9685779323352435 2315 19 -1.7488406127471308 2315 20 -1.4316710829426995 2315 21 -2.0401860904525684 2315 22 -1.5761070156998578 2315 23 -1.7623961359972 2315 24 -1.583072676498837 2315 25 -1.3777839384822563 2316 1 -1.9104168427943016 2316 2 -1.8469883270345662 2316 3 -1.537369860151582 2316 4 -1.6065317090506426 2316 5 -1.7392911564243043 2316 6 -1.9081202372339767 2316 7 -1.955682771838383 2316 8 -2.101609217068681 2316 9 -2.4206357256831836 2316 10 -2.2245877883621112 2316 11 -2.037540960950132 2316 12 -2.136941832637593 2316 13 -2.088214529451392 2316 14 -2.4353699067345147 2316 15 -2.393665201387339 2316 16 -2.467111940541322 2316 17 -2.5129291304414814 2316 18 -2.6082777825278534 2316 19 -2.5005437801033885 2316 20 -2.59006118852124 2316 21 -2.788215409898992 2316 22 -2.578666225287531 2316 23 -2.4947402649742294 2316 24 -2.44150469247829 2316 25 -2.164718593925258 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44168.0 3 4.0 4 768.0 5 5949.0 6 7294.0 7 5877.0 8 4835.0 9 4274.0 10 4107.0 11 3730.0 12 3537.0 13 3481.0 14 3297.0 15 3263.0 16 3384.0 17 3555.0 18 3807.0 19 4338.0 20 5247.0 21 6559.0 22 8419.0 23 10539.0 24 12551.0 25 14628.0 26 16703.0 27 18913.0 28 22192.0 29 26666.0 30 33040.0 31 41891.0 32 53347.0 33 68334.0 34 88583.0 35 119342.0 36 170299.0 37 258211.0 38 262513.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.48857352453797 22.65655779521343 14.772159393467712 23.08270928678089 2 15.094628036315202 23.247739575333267 41.230220124736114 20.42741226361542 3 19.233255048621857 27.45173988698804 31.327167020988465 21.987838043401638 4 13.516912836837594 19.921492677967862 38.88108515224707 27.680509332947473 5 12.01586895126124 39.721736677837285 36.59656271687134 11.665831654030143 6 29.575370368309162 40.033911007720874 18.392677596844866 11.998041027125096 7 26.28629943345614 32.80700778023886 23.866225897769812 17.040466888535185 8 23.465081679522427 40.039698882124 21.092572598866912 15.40264683948666 9 26.10116165607411 15.12460625758267 21.621124257501048 37.15310782884217 10 14.758708710379958 30.33573381714027 35.61642717481236 19.289130297667413 11 32.829565649707455 22.448938704183966 27.097047071001633 17.624448575106946 12 23.255901962312034 25.596651937268344 33.39885503971743 17.748591060702186 13 29.05520370720776 23.312593450055466 26.51328799498384 21.118914847752933 14 19.501500766151324 24.58941338408854 28.87644743237277 27.032638417387368 15 21.41728719358585 34.79143246181302 23.72857837190061 20.062701972700523 16 20.022008812429416 29.512659890386317 30.484134510500567 19.981196786683704 17 20.13415996052373 30.833713626363025 29.338809552960903 19.69331686015234 18 19.851829755594785 28.050687164737738 33.2241794723958 18.87330360727168 19 23.02453539322299 27.241224506453847 29.326269158420796 20.407970941902356 20 23.036036938511252 28.628904496362175 29.85986665627817 18.475191908848398 21 21.508631724230046 27.53633189749526 28.82932819844989 22.125708179824805 22 22.40322933710287 30.093533534424864 28.957403470498537 18.545833657973724 23 20.344749544575908 30.00582497616212 29.98185723985174 19.66756823941023 24 22.413703542095217 27.953106494385427 30.709339222478487 18.92385074104087 25 21.357749313244913 29.121235461643373 30.176486040061086 19.34452918505063 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 247.0 1 247.0 2 432.0 3 617.0 4 617.0 5 617.0 6 1495.0 7 2373.0 8 2373.0 9 2373.0 10 3462.5 11 4552.0 12 4552.0 13 4552.0 14 6732.0 15 8912.0 16 8912.0 17 8912.0 18 15849.5 19 22787.0 20 22787.0 21 22787.0 22 35811.0 23 48835.0 24 48835.0 25 48835.0 26 70616.0 27 92397.0 28 92397.0 29 92397.0 30 116384.0 31 140371.0 32 140371.0 33 140371.0 34 152777.0 35 165183.0 36 165183.0 37 165183.0 38 167871.5 39 170560.0 40 170560.0 41 170560.0 42 171211.0 43 171862.0 44 171862.0 45 171862.0 46 168295.5 47 164729.0 48 164729.0 49 164729.0 50 151402.5 51 138076.0 52 138076.0 53 138076.0 54 114946.5 55 91817.0 56 91817.0 57 91817.0 58 75622.5 59 59428.0 60 59428.0 61 59428.0 62 47145.5 63 34863.0 64 34863.0 65 34863.0 66 26351.5 67 17840.0 68 17840.0 69 17840.0 70 12794.5 71 7749.0 72 7749.0 73 7749.0 74 5356.0 75 2963.0 76 2963.0 77 2963.0 78 2005.0 79 1047.0 80 1047.0 81 1047.0 82 681.0 83 315.0 84 315.0 85 315.0 86 206.0 87 97.0 88 97.0 89 97.0 90 56.5 91 16.0 92 16.0 93 16.0 94 12.0 95 8.0 96 8.0 97 8.0 98 4.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3658233436847241 2 0.0 3 0.0 4 0.0 5 0.026713266475963622 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 2.2261055396636354E-4 17 0.0 18 4.452211079327271E-4 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0060104849570918155 25 2.2261055396636354E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1347645.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.74913443653995 #Duplication Level Percentage of deduplicated Percentage of total 1 90.83096249745995 68.80366789220412 2 6.671500895291789 10.107208364219087 3 1.242055414723941 2.8225386776266856 4 0.39538799421232834 1.1980119331273416 5 0.20639903571841745 0.7817274152103306 6 0.13315986386546066 0.6052046657697682 7 0.09389147953164777 0.4978538813841898 8 0.06601683721113086 0.40005746215849 9 0.04831905407654175 0.3294113870781364 >10 0.2614233281009578 3.6603027344819496 >50 0.023147939929994803 1.219745695471207 >100 0.02238323810608654 3.556267574408081 >500 0.0028221860250978023 1.5697950602979756 >1k 0.0024329189871532776 4.023083607220569 >5k 9.73167594861311E-5 0.4251236493420251 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5767 0.4279316882413395 No Hit CTGTAGGACGTGGAATATGGCAAGA 4659 0.3457141903097626 No Hit CTTTAGGACGTGAAATATGGCGAGG 4217 0.3129162353587183 No Hit TATCAACGCAGAGTACTTTTTTTTT 4174 0.30972548408520045 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4150 0.30794459965346954 No Hit GTCCTACAGTGGACATTTCTAAATT 4100 0.3042344237540302 No Hit GGTATCAACGCAGAGTACTTTTTTT 4067 0.3017857076604002 No Hit CTGAAGGACCTGGAATATGGCGAGA 2462 0.18268906128839568 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2089 0.15501114907857783 No Hit ATTTAGAAATGTCCACTGTAGGACG 1992 0.1478134078336654 No Hit GTCCTACAGTGTGCATTTCTCATTT 1911 0.1418029228765736 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1893 0.1404672595527754 No Hit CTGTAGGACCTGGAATATGGCGAGA 1804 0.13386314645177327 No Hit GAATATGGCAAGAAAACTGAAAATC 1668 0.12377146800529813 No Hit GGAATATGGCGAGAAAACTGAAAAT 1602 0.11887403581803814 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1453 0.10781771163770874 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1452 0.10774350811971996 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1422 0.10551740258005632 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 7.420351798878784E-5 13 0.0 0.0 0.0 0.0 7.420351798878784E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1025 0.0 14.048027 1 ATACGGC 55 1.964496E-4 13.814323 15 TCGAACT 75 1.4819232E-5 12.663599 19 CACCTTT 770 0.0 12.45756 14 ACCTTTT 770 0.0 12.087533 15 TAGGACC 625 0.0 12.008211 4 CCACCTT 780 0.0 11.810803 13 GTACTAG 65 7.772205E-4 11.736507 1 ACTAGCC 65 8.021021E-4 11.692513 3 CCTACAC 140 6.7848305E-10 11.535917 3 TAGAAAT 660 0.0 11.515353 4 GGCGAGG 1175 0.0 11.478071 19 TTCCACC 830 0.0 11.328161 11 TGGTATC 270 0.0 11.259458 2 CTCGAAC 85 5.3345524E-5 11.173764 18 GCCTCGA 85 5.3365693E-5 11.173349 16 GAATACG 60 0.0058871964 11.080238 13 TCCTATA 165 8.0035534E-11 10.939586 2 TCCAACG 105 3.4745572E-6 10.854514 18 GTGGTAT 290 0.0 10.851211 1 >>END_MODULE