FastQCFastQC Report
Thu 26 May 2016
SRR1511772_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511772_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences845261
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT36040.42637717817336895No Hit
GTATCAACGCAGAGTACTTTTTTTT35580.4209350721256512No Hit
GTCCTAAAGTGTGTATTTCTCATTT33040.3908851822099919No Hit
CTGTAGGACGTGGAATATGGCAAGA32390.3831952497512603No Hit
CTTTAGGACGTGAAATATGGCGAGG27620.32676297616949085No Hit
GGTATCAACGCAGAGTACTTTTTTT23940.2832261277877484No Hit
TATCAACGCAGAGTACTTTTTTTTT21910.2592098771858633No Hit
CTGAAGGACCTGGAATATGGCGAGA16990.20100300380592506No Hit
GTCCTACAGTGTGCATTTCTCATTT14320.1694151273985195No Hit
ATTTAGAAATGTCCACTGTAGGACG13090.15486340905353496No Hit
TTTCTAAATTTTCCACCTTTTTCAG12940.15308880925536608No Hit
GTCCTTCAGTGTGCATTTCTCATTT12390.146581943328747No Hit
GGAATATGGCGAGAAAACTGAAAAT12020.14220459715993047No Hit
CTGTAGGACCTGGAATATGGCGAGA12010.14208629050671923No Hit
GAATATGGCAAGAAAACTGAAAATC11500.13605265119294516No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10260.12138262619474931No Hit
GTACTTTTTTTTTTTTTTTTTTTTT9720.11499406692134147No Hit
ATTCCAGGTCCTTCAGTGTGCATTT8770.10375493486627207No Hit
GCCATATTCCACGTCCTACAGTGGA8570.10138880180204693No Hit
ACAGTGGACATTTCTAAATTTTCCA8460.10008742861672311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG608.157076E-817.32355718
CCAACGA350.002248157516.19865219
GGTATCA5800.015.1099331
CGGTAGG450.00842105313.2784251
GCCGGTT655.7307945E-513.08275111
TGCCGGT655.7307945E-513.08275110
TCTAGAC802.1215383E-612.99189953
GTCCTAG1101.9701474E-812.674861
CTAATAC701.1463014E-412.1482713
TAGGACC5050.011.9745324
TAGAAAT4800.011.8108184
CCCTTAT600.00413065911.6186231
GTACTAG958.0423015E-611.5312641
GTCCTAT1301.623448E-811.4909451
GAAATGT4650.011.37902456
CTAGACT752.1785992E-411.3383864
CCGGTTT752.1785992E-411.33838612
GTATTAG802.4338329E-411.2036711
AGTCGTC1355.12955E-911.1984058
GTAAGAC1105.3169606E-711.1665923
Produced by FastQC (version 0.11.5)