FastQCFastQC Report
Thu 26 May 2016
SRR1511772_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511772_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences845261
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55580.6575483785481644No Hit
TATCAACGCAGAGTACTTTTTTTTT42730.5055243291716996No Hit
GGTATCAACGCAGAGTACTTTTTTT42270.5000822231239819No Hit
CTGTAGGACGTGGAATATGGCAAGA31140.3684069180998532No Hit
GTCCTACAGTGGACATTTCTAAATT26850.3176533638722241No Hit
GTCCTAAAGTGTGTATTTCTCATTT26670.31552384411442147No Hit
CTTTAGGACGTGAAATATGGCGAGG24340.28795839391619865No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19360.22904168061699284No Hit
CTGAAGGACCTGGAATATGGCGAGA17150.20289591025730516No Hit
TTTCTAAATTTTCCACCTTTTTCAG16320.19307645804077084No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14410.1704798872774208No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14130.16716730098750562No Hit
GAATATGGCAAGAAAACTGAAAATC12890.15249727598930982No Hit
GGAATATGGCGAGAAAACTGAAAAT12350.14610871671590195No Hit
ATTTAGAAATGTCCACTGTAGGACG12130.1435059703452543No Hit
GTCCTACAGTGTGCATTTCTCATTT10860.1284810253874247No Hit
ACAGTGGACATTTCTAAATTTTCCA10740.12706134554888962No Hit
ATTCCAGGTCCTTCAGTGTGCATTT9910.11724189333235534No Hit
CTGTAGGACCTGGAATATGGCGAGA9910.11724189333235534No Hit
GTCCTTCAGTGTGCATTTCTCATTT8890.10517461470480716No Hit
GCCATATTCCACGTCCTACAGTGGA8510.1006789618827794No Hit
GAAATATGGCGAGGAAAACTGAAAA8460.10008742861672311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10750.014.8954971
GGCGTGC500.001501517713.2965218
AATGCCG802.0021962E-613.05908518
ATGCCGG905.41233E-712.66335419
AGGACGG1001.4376383E-712.3511535
CCACCTT4900.012.21109113
CACCTTT5050.011.660315514
GACAGTC752.076721E-411.3970197
GTTCTAG855.1405048E-511.21334651
TAGGACC3400.011.1775154
GTCGAGG855.330152E-511.17354819
TCCAACT855.330152E-511.17354812
TAGGACT1803.6379788E-1211.084374
TCTAGAC600.005867819311.0843693
GGACTTA600.005883346311.0804346
AGGCGTG600.005883346311.0804347
GCAATTT600.005883346311.08043413
ATGTCCA4550.011.063049
TCCACCT5450.010.9787812
TAGACAG1303.2516255E-810.9625635
Produced by FastQC (version 0.11.5)