##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511772_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 845261 Sequences flagged as poor quality 0 Sequence length 25 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.40804911145788 31.0 28.0 31.0 22.0 34.0 2 28.623370769501964 31.0 28.0 33.0 22.0 34.0 3 28.610187859134633 31.0 28.0 33.0 22.0 34.0 4 32.12066095561016 35.0 32.0 37.0 25.0 37.0 5 31.568883457298988 35.0 32.0 37.0 19.0 37.0 6 31.554375512415692 35.0 32.0 37.0 19.0 37.0 7 31.273468195030883 35.0 32.0 37.0 17.0 37.0 8 31.108830290289035 35.0 32.0 37.0 17.0 37.0 9 32.21138322955868 37.0 32.0 39.0 16.0 39.0 10 31.846311376012853 37.0 31.0 39.0 11.0 39.0 11 31.89212562746891 37.0 32.0 39.0 10.0 39.0 12 31.600651159819275 37.0 31.0 39.0 10.0 39.0 13 31.59944561502305 37.0 31.0 39.0 10.0 39.0 14 32.065479183352835 37.0 31.0 40.0 10.0 41.0 15 32.00123630452606 37.0 31.0 40.0 8.0 41.0 16 31.81178476233968 37.0 31.0 40.0 2.0 41.0 17 31.7501280669521 37.0 31.0 40.0 2.0 41.0 18 31.589249947649307 37.0 30.0 40.0 2.0 41.0 19 31.643175303249528 37.0 30.0 40.0 2.0 41.0 20 31.452431852410083 37.0 30.0 40.0 2.0 41.0 21 31.235548546543612 37.0 30.0 40.0 2.0 41.0 22 31.291153856619434 37.0 30.0 40.0 2.0 41.0 23 31.072620172940667 37.0 30.0 40.0 2.0 41.0 24 30.82659793838826 37.0 30.0 40.0 2.0 41.0 25 30.252466397952823 36.0 29.0 39.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.047013525472827666 1101 2 0.1506416330157272 1101 3 0.17996620793415374 1101 4 0.2632885141239214 1101 5 0.25758604337783453 1101 6 0.21370750696308605 1101 7 0.1691730145862529 1101 8 -0.022515278427480467 1101 9 0.24281292053770898 1101 10 -0.027216019557059212 1101 11 -0.4008258379025307 1101 12 -0.2881069172502677 1101 13 -0.15012908829713112 1101 14 -0.3394241066460708 1101 15 -0.35010706700400007 1101 16 -0.17367639483707364 1101 17 -0.5487809888291686 1101 18 -0.7945552652499579 1101 19 -0.5903421575035459 1101 20 -0.13414599053410825 1101 21 -0.20245694920492951 1101 22 -0.5637302268365687 1101 23 -0.6646417901823156 1101 24 -0.3999717953558459 1101 25 -0.35567325883185674 1102 1 0.9081339400971338 1102 2 1.017927628444106 1102 3 1.085041444155106 1102 4 1.1955856941407887 1102 5 1.103078593097301 1102 6 1.2424781801164109 1102 7 1.2777075279038534 1102 8 1.1223245313733976 1102 9 1.4201344866245762 1102 10 1.391059475804088 1102 11 1.4749531106239147 1102 12 1.6000630884948244 1102 13 1.7584726547873721 1102 14 1.9427123331099487 1102 15 1.9813491297469703 1102 16 1.7970713224067545 1102 17 2.1253355509158496 1102 18 2.060037750188439 1102 19 2.1477025564032353 1102 20 2.2689514681807026 1102 21 2.2467650190381754 1102 22 1.8551772283333996 1102 23 1.8525898102528409 1102 24 2.152088491409671 1102 25 2.390205556457417 1103 1 0.352455877870792 1103 2 0.4587296676923138 1103 3 0.3558553139700855 1103 4 0.4479097297056711 1103 5 0.6376482409854844 1103 6 0.4662917694127948 1103 7 0.7843009344972636 1103 8 0.5601311199014063 1103 9 0.706915962640231 1103 10 0.7912948674934164 1103 11 0.6003766327178042 1103 12 0.42942606735835653 1103 13 0.4916482472304402 1103 14 0.5314757339710177 1103 15 0.5653656681475248 1103 16 0.4332008976040278 1103 17 0.1911939987942759 1103 18 0.6887823685002097 1103 19 0.8313349062953002 1103 20 0.7231476604457043 1103 21 0.7098207202736759 1103 22 0.4979599727809614 1103 23 0.7529105771662863 1103 24 0.8865532497368029 1103 25 0.539241426248001 1104 1 0.38673187724770486 1104 2 0.23816090394053901 1104 3 0.2458593543151224 1104 4 0.31891806352579266 1104 5 0.13350080246688023 1104 6 0.37901423178403704 1104 7 0.2788184783569143 1104 8 0.5013538354027034 1104 9 0.5460140894778576 1104 10 0.6461491229989953 1104 11 0.5564573519965776 1104 12 0.9670815963028012 1104 13 1.0275781107349324 1104 14 0.7926207580183693 1104 15 0.7019390661263749 1104 16 0.9021754288266486 1104 17 1.0522754969347403 1104 18 0.7100136514801285 1104 19 0.5352628031740103 1104 20 0.9088806179782694 1104 21 0.7183261333624458 1104 22 0.6882310166503451 1104 23 0.812217244222385 1104 24 0.7069732995839502 1104 25 0.8121642994687903 1105 1 0.18339149504219066 1105 2 0.22555125481773075 1105 3 0.19256204855071246 1105 4 0.4931818479869463 1105 5 0.12515963705334698 1105 6 0.40358738341311096 1105 7 0.23296164878297887 1105 8 0.2353568115931779 1105 9 0.5165458877234741 1105 10 0.4753314538260618 1105 11 0.6710211051294124 1105 12 0.6413062203629529 1105 13 0.5765873525895664 1105 14 0.3078737342088509 1105 15 0.4018333330436725 1105 16 0.43436527845070927 1105 17 0.5599509355312335 1105 18 0.7258344534851453 1105 19 0.9912465124471943 1105 20 0.7373580615872939 1105 21 0.35229386519202066 1105 22 0.7611868437180185 1105 23 0.9706014860770189 1105 24 0.8804538760250544 1105 25 1.016562012501371 1106 1 0.5754298496052321 1106 2 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0.6603577610764724 1107 15 0.6276995682992386 1107 16 0.8633053177691856 1107 17 1.0633913025037103 1107 18 0.6772597483737393 1107 19 0.621734716903287 1107 20 0.6316896474326441 1107 21 0.8440001233833705 1107 22 0.9627964439801353 1107 23 0.955314379261818 1107 24 0.699056431726234 1107 25 0.42839236964422867 1108 1 -0.252128378229699 1108 2 -0.04763422905323722 1108 3 -0.018627467578319568 1108 4 -0.08213497228955191 1108 5 -0.07047688368398042 1108 6 0.08701912304444903 1108 7 -0.007173719880615437 1108 8 -0.2592068404805552 1108 9 -0.11885877817514512 1108 10 0.018390209586431894 1108 11 0.12591021803263658 1108 12 0.00658559824313798 1108 13 -0.11699703681135531 1108 14 0.21408773190470143 1108 15 0.4044615012464341 1108 16 -0.04849762477509856 1108 17 0.019628354886073396 1108 18 -0.21224158338121413 1108 19 0.11250227281585623 1108 20 0.007519351909873961 1108 21 0.10006172233487831 1108 22 0.25535447044612525 1108 23 0.34131721188347086 1108 24 0.5217269174997092 1108 25 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1110 11 -0.10738868551098335 1110 12 -0.30041689129550164 1110 13 -0.4190946055385112 1110 14 -0.5266321155448459 1110 15 -0.25262838698916923 1110 16 -0.23062856391382525 1110 17 -0.3164858460779634 1110 18 -0.5658937895362008 1110 19 -0.4340203184230873 1110 20 -0.5405976034373339 1110 21 -0.6363093778293276 1110 22 -0.45620334511614047 1110 23 -0.6193692651545071 1110 24 -0.4376803604281463 1110 25 -0.174768549896001 1111 1 -0.19951477058541656 1111 2 -0.29548871017643563 1111 3 -0.4466356302290855 1111 4 -0.44175001565699645 1111 5 -0.38903804172645096 1111 6 -0.3675606725128766 1111 7 -0.6159324377314412 1111 8 -0.664583543049261 1111 9 -1.1589299728366207 1111 10 -0.916416129104423 1111 11 -1.218506055647385 1111 12 -1.203441341730727 1111 13 -1.009019363113051 1111 14 -1.1659070521722157 1111 15 -0.9483096595384239 1111 16 -1.2632041081137189 1111 17 -1.3446581659856847 1111 18 -1.3525848664015037 1111 19 -2.020390843778845 1111 20 -1.4513608915715643 1111 21 -1.731285168813649 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0.30998318187353036 2313 23 0.3282002757367266 2313 24 0.16690279103409722 2313 25 -0.07462834381952277 2314 1 -0.9070206727318961 2314 2 -1.0138382800659897 2314 3 -0.9843171042732095 2314 4 -1.16252828490342 2314 5 -1.1685799808868538 2314 6 -1.1982450501417041 2314 7 -1.1691874375957596 2314 8 -1.3693825987058261 2314 9 -1.3555107983230386 2314 10 -1.3996893102935353 2314 11 -1.0707407304187626 2314 12 -1.415078746414455 2314 13 -1.3817650145200666 2314 14 -1.3978831179506201 2314 15 -1.574988718451344 2314 16 -1.702200336167234 2314 17 -1.5691188319106253 2314 18 -1.746300098009872 2314 19 -1.8724993347288859 2314 20 -1.793123665987455 2314 21 -1.8295063839774173 2314 22 -1.781597624720522 2314 23 -1.5491607647535446 2314 24 -1.5867180508852456 2314 25 -1.6163750722162362 2315 1 -0.5415709965643245 2315 2 -0.6626317651587676 2315 3 -0.8170091491998335 2315 4 -1.1095072152999457 2315 5 -0.7758627945397372 2315 6 -1.109303176012773 2315 7 -0.8431961277824058 2315 8 -1.1072928547657277 2315 9 -0.9813518061660673 2315 10 -1.1276618733520465 2315 11 -1.0659283346053563 2315 12 -1.1763951183981654 2315 13 -1.1017073532187247 2315 14 -1.0389989051230302 2315 15 -1.0494988706662483 2315 16 -1.3524244227411693 2315 17 -1.1694850221655955 2315 18 -0.9258043747841178 2315 19 -1.0502068197670091 2315 20 -1.1448603369354515 2315 21 -1.0075008091406978 2315 22 -1.170062631689067 2315 23 -1.1372062925867361 2315 24 -1.2916493218933631 2315 25 -1.1895571015661126 2316 1 -1.854229693850538 2316 2 -1.7745621067300306 2316 3 -1.4199600839419482 2316 4 -1.5727136002770052 2316 5 -1.6561956272410043 2316 6 -1.4511330454174285 2316 7 -1.6389155104845976 2316 8 -1.5735655519411118 2316 9 -1.530640856532873 2316 10 -1.5679888519262235 2316 11 -1.4467790870454031 2316 12 -1.6604163353461487 2316 13 -1.822063375083605 2316 14 -1.5951765011513181 2316 15 -1.9308490710848467 2316 16 -1.756109112635837 2316 17 -1.5570381817601806 2316 18 -1.6972632317800134 2316 19 -1.5534827153987223 2316 20 -1.8828483643930127 2316 21 -2.0278964742448444 2316 22 -1.8926096884982577 2316 23 -1.5566957047158638 2316 24 -1.46977392670426 2316 25 -1.2546487428104278 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 42132.0 3 3.0 4 673.0 5 4922.0 6 6033.0 7 4844.0 8 3915.0 9 3638.0 10 3359.0 11 3129.0 12 2905.0 13 2854.0 14 2688.0 15 2736.0 16 2876.0 17 2894.0 18 3098.0 19 3535.0 20 4501.0 21 5542.0 22 7033.0 23 8601.0 24 10228.0 25 11730.0 26 13133.0 27 15268.0 28 17549.0 29 21063.0 30 25981.0 31 32283.0 32 40914.0 33 51031.0 34 64426.0 35 81627.0 36 104570.0 37 131236.0 38 102311.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.51496200587155 24.106832295187253 15.902094930119532 20.476110768821666 2 16.587657540097084 25.0031647029734 40.335233732539415 18.073944024390098 3 20.48881942973827 28.52728328883031 31.570603636036683 19.413293645394734 4 15.57720041501974 21.50081454130736 37.791995608457036 25.129989435215865 5 12.179550897920159 40.57491503602094 36.29571251408177 10.949821551977127 6 29.03233439138917 41.05962537015194 18.1832593719573 11.72478086650159 7 25.953285434913003 33.917097795828745 23.953193155723497 16.176423613534755 8 23.83453158255261 40.62260059318956 20.96216434923651 14.580703475021325 9 26.641711849949306 15.496515277529662 22.158481226508737 35.703291646012296 10 15.549516658168304 31.167532868545926 34.960325863845604 18.32262460944016 11 32.72953561089415 22.816857751629378 27.312628880310342 17.14097775716613 12 23.739886259983603 26.51583357093253 33.15697754894642 16.587302620137446 13 29.64812052135376 23.892028616013278 26.527072702987596 19.932778159645366 14 19.813406746555206 25.650302096038974 29.082141492391106 25.454149665014715 15 21.81432717231719 35.696429860126045 23.932844411371164 18.556398556185606 16 19.93549921266922 30.131639813028166 30.959194852240906 18.973666122061704 17 20.114615485631067 31.621238883611095 29.631084363291336 18.6330612674665 18 19.82324965099496 28.055036320185504 34.151621986134444 17.97009204268509 19 23.046017738899582 27.47530052847582 30.33335265675336 19.14532907587124 20 23.128595782841042 28.85250827850806 30.80232023008278 17.216575708568122 21 21.4980934882835 27.835544287504092 29.773880493717325 20.892481730495078 22 22.44797760691668 30.399367769245238 29.95110386022779 17.201550763610292 23 20.18772899731562 30.284846928936744 31.087675877628328 18.439748196119304 24 22.39430020917532 28.32669209582312 31.609253878261857 17.669753816739707 25 21.19830442904618 29.48426580665617 31.29104501449848 18.026384749799174 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 261.0 1 261.0 2 434.5 3 608.0 4 608.0 5 608.0 6 1390.0 7 2172.0 8 2172.0 9 2172.0 10 2891.5 11 3611.0 12 3611.0 13 3611.0 14 5134.0 15 6657.0 16 6657.0 17 6657.0 18 11324.5 19 15992.0 20 15992.0 21 15992.0 22 25446.5 23 34901.0 24 34901.0 25 34901.0 26 50242.5 27 65584.0 28 65584.0 29 65584.0 30 80907.0 31 96230.0 32 96230.0 33 96230.0 34 103069.5 35 109909.0 36 109909.0 37 109909.0 38 108737.5 39 107566.0 40 107566.0 41 107566.0 42 106976.0 43 106386.0 44 106386.0 45 106386.0 46 102060.0 47 97734.0 48 97734.0 49 97734.0 50 88746.0 51 79758.0 52 79758.0 53 79758.0 54 65581.5 55 51405.0 56 51405.0 57 51405.0 58 41846.0 59 32287.0 60 32287.0 61 32287.0 62 25195.0 63 18103.0 64 18103.0 65 18103.0 66 13788.5 67 9474.0 68 9474.0 69 9474.0 70 6869.5 71 4265.0 72 4265.0 73 4265.0 74 2939.5 75 1614.0 76 1614.0 77 1614.0 78 1071.0 79 528.0 80 528.0 81 528.0 82 341.5 83 155.0 84 155.0 85 155.0 86 99.5 87 44.0 88 44.0 89 44.0 90 28.5 91 13.0 92 13.0 93 13.0 94 8.5 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34237945439337675 2 0.0 3 0.0 4 0.0 5 0.02330641068261756 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.1830665321125664E-4 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.004377346168816495 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 845261.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.47941440463518 #Duplication Level Percentage of deduplicated Percentage of total 1 91.4163577217059 68.08636790117303 2 6.048671793632606 9.010030662311816 3 1.1800702769057467 2.636728295407671 4 0.43361911828529365 1.2918279201817162 5 0.21756769024529554 0.8102157081419336 6 0.14005594653809 0.62587709292263 7 0.09060116631685121 0.4723545268159225 8 0.06729276706928371 0.40095407079902157 9 0.04997126183569821 0.3349647286725148 >10 0.29356900786167506 4.0548801460210635 >50 0.02748076943662254 1.4386967738108116 >100 0.0279519045774096 4.108402404887313 >500 0.004105929426237569 2.142532425350555 >1k 0.002526725800761581 3.932445603270227 >5k 1.579203625475988E-4 0.6537217402337716 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5558 0.6575483785481644 No Hit TATCAACGCAGAGTACTTTTTTTTT 4273 0.5055243291716996 No Hit GGTATCAACGCAGAGTACTTTTTTT 4227 0.5000822231239819 No Hit CTGTAGGACGTGGAATATGGCAAGA 3114 0.3684069180998532 No Hit GTCCTACAGTGGACATTTCTAAATT 2685 0.3176533638722241 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2667 0.31552384411442147 No Hit CTTTAGGACGTGAAATATGGCGAGG 2434 0.28795839391619865 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1936 0.22904168061699284 No Hit CTGAAGGACCTGGAATATGGCGAGA 1715 0.20289591025730516 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1632 0.19307645804077084 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1441 0.1704798872774208 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1413 0.16716730098750562 No Hit GAATATGGCAAGAAAACTGAAAATC 1289 0.15249727598930982 No Hit GGAATATGGCGAGAAAACTGAAAAT 1235 0.14610871671590195 No Hit ATTTAGAAATGTCCACTGTAGGACG 1213 0.1435059703452543 No Hit GTCCTACAGTGTGCATTTCTCATTT 1086 0.1284810253874247 No Hit ACAGTGGACATTTCTAAATTTTCCA 1074 0.12706134554888962 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 991 0.11724189333235534 No Hit CTGTAGGACCTGGAATATGGCGAGA 991 0.11724189333235534 No Hit GTCCTTCAGTGTGCATTTCTCATTT 889 0.10517461470480716 No Hit GCCATATTCCACGTCCTACAGTGGA 851 0.1006789618827794 No Hit GAAATATGGCGAGGAAAACTGAAAA 846 0.10008742861672311 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.1830665321125664E-4 0.0 3 0.0 1.1830665321125664E-4 0.0 1.1830665321125664E-4 0.0 4 0.0 1.1830665321125664E-4 0.0 3.5491995963376994E-4 0.0 5 0.0 1.1830665321125664E-4 0.0 3.5491995963376994E-4 0.0 6 0.0 1.1830665321125664E-4 0.0 3.5491995963376994E-4 0.0 7 0.0 1.1830665321125664E-4 0.0 3.5491995963376994E-4 0.0 8 0.0 1.1830665321125664E-4 0.0 3.5491995963376994E-4 0.0 9 0.0 1.1830665321125664E-4 0.0 7.098399192675399E-4 0.0 10 0.0 1.1830665321125664E-4 0.0 9.464532256900531E-4 0.0 11 0.0 1.1830665321125664E-4 0.0 0.0011830665321125664 0.0 12 0.0 1.1830665321125664E-4 0.0 0.0011830665321125664 0.0 13 0.0 1.1830665321125664E-4 0.0 0.0011830665321125664 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1075 0.0 14.895497 1 GGCGTGC 50 0.0015015177 13.296521 8 AATGCCG 80 2.0021962E-6 13.059085 18 ATGCCGG 90 5.41233E-7 12.663354 19 AGGACGG 100 1.4376383E-7 12.351153 5 CCACCTT 490 0.0 12.211091 13 CACCTTT 505 0.0 11.6603155 14 GACAGTC 75 2.076721E-4 11.397019 7 GTTCTAG 85 5.1405048E-5 11.2133465 1 TAGGACC 340 0.0 11.177515 4 GTCGAGG 85 5.330152E-5 11.173548 19 TCCAACT 85 5.330152E-5 11.173548 12 TAGGACT 180 3.6379788E-12 11.08437 4 TCTAGAC 60 0.0058678193 11.084369 3 GGACTTA 60 0.0058833463 11.080434 6 AGGCGTG 60 0.0058833463 11.080434 7 GCAATTT 60 0.0058833463 11.080434 13 ATGTCCA 455 0.0 11.06304 9 TCCACCT 545 0.0 10.97878 12 TAGACAG 130 3.2516255E-8 10.962563 5 >>END_MODULE