FastQCFastQC Report
Thu 26 May 2016
SRR1511771_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511771_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1874449
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60800.32436198584223946No Hit
GTCCTACAGTGGACATTTCTAAATT40380.21542330572877685No Hit
TATCAACGCAGAGTACTTTTTTTTT38930.20768769915852606No Hit
GTCCTAAAGTGTGTATTTCTCATTT38000.2027262411513997No Hit
CTGTAGGACGTGGAATATGGCAAGA37530.20021883764242185No Hit
GGTATCAACGCAGAGTACTTTTTTT36560.19504398359197825No Hit
CTTTAGGACGTGAAATATGGCGAGG33370.17802564913742652No Hit
CTGAAGGACCTGGAATATGGCGAGA19060.10168321464067574No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACACG350.002237064816.2121374
CCGTTTG400.00544062214.185629
TCCAACG2700.014.01048818
CGGTAGG801.2851069E-613.5866291
CCAACGT1403.6379788E-1212.83460819
GCGCCAC905.7120633E-712.6094413
TAGGACC5550.012.6094394
GGTATCA11700.012.3303661
GGCGAGG8750.012.10506219
GTCCTAT1900.011.9614331
GCACCGT658.335398E-411.63948256
CGTTAGG600.00438282111.5280481
CCGTAGG600.00438282111.5280481
CGTAGGA600.00438282111.5280482
ATTTAGA6950.011.3740241
CCAACGA1950.011.15450419
TGTCCAC7400.011.11845910
TAGAAAT8350.010.9860694
GAAATGT8100.010.9748826
ACACCGT951.43050165E-510.9503036
Produced by FastQC (version 0.11.5)