##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511771_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1874449 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.559409191714472 31.0 30.0 34.0 25.0 34.0 2 26.870868185797534 31.0 26.0 34.0 10.0 34.0 3 28.553173759328743 31.0 28.0 34.0 16.0 34.0 4 33.09317511439362 35.0 33.0 37.0 27.0 37.0 5 33.56934277753089 35.0 35.0 37.0 28.0 37.0 6 33.82788968918333 37.0 35.0 37.0 30.0 37.0 7 33.873280094577126 37.0 35.0 37.0 30.0 37.0 8 33.89645223743084 37.0 35.0 37.0 30.0 37.0 9 35.50439942617804 39.0 35.0 39.0 30.0 39.0 10 35.37756962179286 39.0 35.0 39.0 30.0 39.0 11 35.395148654351225 39.0 35.0 39.0 30.0 39.0 12 35.309667000809306 39.0 35.0 39.0 30.0 39.0 13 35.30922100307877 39.0 35.0 39.0 30.0 39.0 14 36.46686252866842 40.0 37.0 41.0 30.0 41.0 15 36.4154495534421 40.0 37.0 41.0 29.0 41.0 16 36.365446592572006 40.0 36.0 41.0 29.0 41.0 17 36.343341429934874 40.0 36.0 41.0 29.0 41.0 18 36.28921512401778 40.0 36.0 41.0 29.0 41.0 19 36.32643352793274 40.0 37.0 41.0 29.0 41.0 20 36.25847969189879 40.0 37.0 41.0 29.0 41.0 21 36.1742282665466 40.0 37.0 41.0 28.0 41.0 22 36.07377367962532 40.0 36.0 41.0 28.0 41.0 23 35.96959479825805 40.0 36.0 41.0 27.0 41.0 24 35.87573521605549 40.0 36.0 41.0 27.0 41.0 25 35.773348861452085 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8844096029513686 1101 2 3.5853545547866936 1101 3 2.1453394112651623 1101 4 1.0799356273189389 1101 5 0.6079198909371542 1101 6 0.42868493164445454 1101 7 0.44587709220933647 1101 8 0.2866144620263782 1101 9 0.2566954808831525 1101 10 0.41057627299474575 1101 11 0.2799794946277103 1101 12 0.35983902736758466 1101 13 0.4527511741701318 1101 14 0.37666900381501733 1101 15 0.26205425792970516 1101 16 0.5366336510189669 1101 17 0.501294083150789 1101 18 0.3764394765468211 1101 19 0.42138078302470916 1101 20 0.5469170134329673 1101 21 0.6148348244587396 1101 22 0.5308716333865675 1101 23 0.5145892829657157 1101 24 0.531866219765945 1101 25 0.24140464146706364 1102 1 1.5404901210578856 1102 2 4.17069272846798 1102 3 2.7320114692411757 1102 4 1.6879502565269888 1102 5 1.281264006889181 1102 6 0.983525448601732 1102 7 0.7901464176872395 1102 8 0.7994889594431172 1102 9 1.0060262148955559 1102 10 0.9151035362883775 1102 11 0.8692436907564485 1102 12 0.8861588418659849 1102 13 0.9508815390721992 1102 14 1.0648725992606316 1102 15 0.9134473790245963 1102 16 0.8167434376819855 1102 17 1.0670435512026373 1102 18 1.0285622834754093 1102 19 0.9748143203489619 1102 20 0.9636880416519915 1102 21 1.136649769033042 1102 22 0.7698325472388561 1102 23 0.7105959114031037 1102 24 0.5805253216988717 1102 25 0.6224578227620938 1103 1 1.3261201524070216 1103 2 3.9216758995743497 1103 3 2.5329521875442325 1103 4 1.4443436161433425 1103 5 1.0835247102462446 1103 6 0.9137257948993849 1103 7 0.7349418993739221 1103 8 0.6968471984210538 1103 9 0.7759251015168545 1103 10 0.5793889656559514 1103 11 0.7934147621219267 1103 12 0.9241559324447124 1103 13 0.837196757852702 1103 14 1.1052153802082358 1103 15 0.8001244438467268 1103 16 0.817304319397806 1103 17 0.9906858958998583 1103 18 1.1643620039300089 1103 19 1.08254675453491 1103 20 0.9871766933623505 1103 21 0.8687900822639065 1103 22 1.0632436930737938 1103 23 0.9803266963200201 1103 24 1.133089462878253 1103 25 0.835937625728981 1104 1 1.2093826953435958 1104 2 3.852065595403019 1104 3 2.3026545040728053 1104 4 1.4393693401911563 1104 5 0.8847802923797801 1104 6 0.8865344001011906 1104 7 0.7142686781531751 1104 8 0.6929476631819895 1104 9 0.666422817124392 1104 10 0.6501247916601329 1104 11 0.6627284136027711 1104 12 0.6767776024917396 1104 13 0.5365990564941967 1104 14 0.7165215312418951 1104 15 0.7631777685700101 1104 16 0.5534721487247367 1104 17 0.5204928973277987 1104 18 0.37795952035429536 1104 19 0.6259918972829084 1104 20 0.5715870411902841 1104 21 0.6716759657189613 1104 22 0.7042840845280196 1104 23 0.6834132788870946 1104 24 0.6945581134950558 1104 25 0.5867425443730809 1105 1 1.3021978049580305 1105 2 3.904219616866701 1105 3 2.3870465648922377 1105 4 1.4624955200982939 1105 5 1.0245493282149454 1105 6 0.7071374920956757 1105 7 0.6991020880210286 1105 8 0.6753404723389238 1105 9 0.7156175039281436 1105 10 0.4617946047458332 1105 11 0.49658473280273085 1105 12 0.6872203121537979 1105 13 0.6796038016342507 1105 14 0.5377326474553072 1105 15 0.6234725114990738 1105 16 0.7253261548815146 1105 17 0.6700455955947149 1105 18 0.7637275017138876 1105 19 0.8221923837022871 1105 20 0.9494039366101177 1105 21 0.7807801717158682 1105 22 0.849406284640267 1105 23 0.9312745643268272 1105 24 0.8744184805205819 1105 25 0.8575556810977432 1106 1 1.3404329571749471 1106 2 3.8412115235584565 1106 3 2.391325404087489 1106 4 1.5708279488144612 1106 5 0.9538459582144583 1106 6 0.7975132700664389 1106 7 0.7394118289386711 1106 8 0.6093788039836383 1106 9 0.5931941551560129 1106 10 0.6515455937939336 1106 11 0.6723880328461505 1106 12 0.5171553003334637 1106 13 0.6775322283240754 1106 14 0.8021688297296379 1106 15 0.9014146280620068 1106 16 0.6798088344781803 1106 17 0.7289241115401026 1106 18 0.8450103694709199 1106 19 0.7920972782842313 1106 20 0.7461560594450702 1106 21 0.7689221483646804 1106 22 0.7383328659110759 1106 23 0.6869706370821689 1106 24 0.7273925602233859 1106 25 0.6320873909448039 1107 1 1.4033581272429743 1107 2 3.7834592559847593 1107 3 2.408190483452568 1107 4 1.579118765140997 1107 5 1.1665018539060696 1107 6 0.9867848120165519 1107 7 0.7824957291654258 1107 8 0.7024910488815976 1107 9 0.8574373524349213 1107 10 0.8345813080796489 1107 11 0.9130342913495468 1107 12 0.7860045066826729 1107 13 0.907265788414783 1107 14 0.9801025031990207 1107 15 1.07297075051099 1107 16 1.0711070204192197 1107 17 1.0109535899981523 1107 18 0.8438765826228476 1107 19 0.9598268701209633 1107 20 0.9792201184019831 1107 21 1.0693048014259077 1107 22 1.0394241357523413 1107 23 1.2812166688282005 1107 24 1.2304396123775803 1107 25 1.1274813818745102 1108 1 1.4823207955982873 1108 2 4.126274861631845 1108 3 2.64804873849474 1108 4 1.5456541544736453 1108 5 1.2624947783848768 1108 6 1.0527885497482643 1108 7 0.8964530340979238 1108 8 0.9170961614764437 1108 9 0.8990739308550744 1108 10 0.7727513501846772 1108 11 0.7427625898545784 1108 12 0.8414952567841993 1108 13 0.8761681198064153 1108 14 0.8314434206220653 1108 15 0.8845840730310712 1108 16 0.9488928834488846 1108 17 0.9191341367586787 1108 18 1.112979996080405 1108 19 1.042820437716756 1108 20 0.9362664462780828 1108 21 0.9091584892265558 1108 22 1.1283088277165874 1108 23 1.119392203966953 1108 24 1.0840906899379874 1108 25 1.2013423180108944 1109 1 1.0181220168837122 1109 2 3.160931181978853 1109 3 1.8194035451582415 1109 4 1.0454723622827657 1109 5 0.7966317130999698 1109 6 0.5312716606905923 1109 7 0.4141885300700494 1109 8 0.4559068449082275 1109 9 0.4117039397064346 1109 10 0.31747526141695204 1109 11 0.3920613398347257 1109 12 0.40034495550587934 1109 13 0.31310644474804405 1109 14 0.4603358166835676 1109 15 0.43917818839330636 1109 16 0.42399832725141806 1109 17 0.43581930993776297 1109 18 0.2653183865769648 1109 19 0.377533793739218 1109 20 0.42063130710381813 1109 21 0.39499905790834333 1109 22 0.40456364867510786 1109 23 0.46466308010712254 1109 24 0.4177511240733338 1109 25 0.3853905225422736 1110 1 0.9274389252063315 1110 2 3.4762656218849024 1110 3 2.0953418266074166 1110 4 1.221349890720063 1110 5 0.6536302430940566 1110 6 0.39242661884153307 1110 7 0.1427234603396954 1110 8 0.17428897050422876 1110 9 0.37749077257598174 1110 10 0.31533127677878525 1110 11 0.2105907372407927 1110 12 0.24274323489379412 1110 13 0.1186892125901764 1110 14 0.15357189095412593 1110 15 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-1.3194795186644512 2315 15 -1.3113745298897257 2315 16 -1.4891952099256542 2315 17 -1.3516720572756853 2315 18 -1.2628770641094818 2315 19 -1.396351422091442 2315 20 -1.493399775326651 2315 21 -1.5564609277513384 2315 22 -1.515886048022658 2315 23 -1.4103594641125383 2315 24 -1.3779808702433272 2315 25 -1.4287256780638913 2316 1 -0.44934364617682476 2316 2 1.894879270359148 2316 3 0.5406556456484708 2316 4 -0.404342239075433 2316 5 -0.8602072053385186 2316 6 -0.9284197581992331 2316 7 -1.3061942104968338 2316 8 -1.2765275017525113 2316 9 -1.4860726653458514 2316 10 -1.4957095678357604 2316 11 -1.4704742469832226 2316 12 -1.5609004789657703 2316 13 -1.601243549002561 2316 14 -1.89807933739867 2316 15 -1.6925840013938043 2316 16 -1.9537899433333905 2316 17 -1.7713049974212467 2316 18 -1.9908428199724 2316 19 -1.920481676978902 2316 20 -1.6978460631518573 2316 21 -2.133591218037054 2316 22 -1.8883523178281507 2316 23 -2.0690072574989458 2316 24 -2.1066090075805377 2316 25 -1.8909742753246022 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 56553.0 3 5.0 4 480.0 5 2440.0 6 3073.0 7 2810.0 8 2536.0 9 2447.0 10 2466.0 11 2282.0 12 2327.0 13 2333.0 14 2298.0 15 2303.0 16 2473.0 17 2784.0 18 3163.0 19 3899.0 20 5047.0 21 6552.0 22 8084.0 23 9492.0 24 11289.0 25 12799.0 26 14653.0 27 17092.0 28 19449.0 29 24118.0 30 30749.0 31 39812.0 32 51507.0 33 69655.0 34 96073.0 35 139320.0 36 225635.0 37 286383.0 38 710068.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.209470623100444 19.43904582093191 13.50076742552078 24.850716130446866 2 15.881368280407514 21.354868954045983 40.87689500883227 21.886867756714228 3 18.459077841008213 25.302742299203658 30.165878079371588 26.07230178041654 4 11.856871005826246 17.414130765894402 39.36127363294493 31.36772459533442 5 12.615120496743309 38.186635112505066 36.43177275028555 12.76647164046608 6 30.5025636867154 38.12965836893935 18.72123488022347 12.646543064121776 7 26.914202520313967 32.14208548752193 23.05877620570098 17.884935786463117 8 24.076995426389303 38.27407414125431 21.076753755370245 16.572176676986146 9 26.11839532577307 15.69309167654068 20.913772527286685 37.27474047039957 10 14.689863527895398 29.643644612363417 35.095753472087 20.570738387654185 11 33.669094224489434 22.69637637513744 25.489357139084607 18.14517226128852 12 22.967922840258655 24.886086524626705 32.706197928031116 19.439792707083523 13 28.73345713860446 22.471723690535192 26.30527691070816 22.489542260152184 14 20.353181121492238 22.834230219120393 28.3930371005026 28.41955155888477 15 22.314717551664515 31.846585316538356 24.008121853408653 21.830575278388476 16 21.24565672365586 28.583599767184914 28.857493588782624 21.313249920376602 17 20.923748792311766 29.056912191262608 28.744553732856964 21.274785283568665 18 20.74657672734761 27.866429014606425 31.078893050704497 20.308101207341466 19 22.85280786663594 27.147672274717678 28.41257799629544 21.58694186235094 20 22.827881686831702 27.917910810056718 29.092976122583224 20.161231380528356 21 22.364445136797897 27.235222818072984 28.03620700325003 22.364125041879085 22 22.40372380863465 28.66761806954133 28.31716393027195 20.611494191552076 23 21.690844606057503 28.19290709312028 28.726400901391138 21.389847399431083 24 22.408238367648305 27.315013638674618 29.433342811674258 20.843405182002815 25 22.176970405703223 27.776856025424006 29.070836283089058 20.975337285783716 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 281.0 1 281.0 2 480.0 3 679.0 4 679.0 5 679.0 6 1709.0 7 2739.0 8 2739.0 9 2739.0 10 3855.5 11 4972.0 12 4972.0 13 4972.0 14 7840.0 15 10708.0 16 10708.0 17 10708.0 18 18460.5 19 26213.0 20 26213.0 21 26213.0 22 41118.5 23 56024.0 24 56024.0 25 56024.0 26 80122.5 27 104221.0 28 104221.0 29 104221.0 30 133541.5 31 162862.0 32 162862.0 33 162862.0 34 179916.0 35 196970.0 36 196970.0 37 196970.0 38 207142.5 39 217315.0 40 217315.0 41 217315.0 42 225944.5 43 234574.0 44 234574.0 45 234574.0 46 237520.0 47 240466.0 48 240466.0 49 240466.0 50 227931.5 51 215397.0 52 215397.0 53 215397.0 54 188645.0 55 161893.0 56 161893.0 57 161893.0 58 136938.5 59 111984.0 60 111984.0 61 111984.0 62 90069.0 63 68154.0 64 68154.0 65 68154.0 66 51520.0 67 34886.0 68 34886.0 69 34886.0 70 25191.5 71 15497.0 72 15497.0 73 15497.0 74 10676.0 75 5855.0 76 5855.0 77 5855.0 78 3882.5 79 1910.0 80 1910.0 81 1910.0 82 1250.5 83 591.0 84 591.0 85 591.0 86 384.0 87 177.0 88 177.0 89 177.0 90 115.0 91 53.0 92 53.0 93 53.0 94 38.0 95 23.0 96 23.0 97 23.0 98 14.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.292461411326742 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 5.334901082931571E-5 20 0.0 21 2.6674505414657853E-4 22 0.001280376259903577 23 6.935371407811043E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1874449.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.10244321729826 #Duplication Level Percentage of deduplicated Percentage of total 1 85.1135392914678 63.07121212369277 2 10.805785469373657 16.014702083251358 3 2.362709128757715 5.252475571582823 4 0.72891155809525 2.1605650937674263 5 0.3247475900262174 1.2032294924936116 6 0.17154395748502252 0.7627095821282703 7 0.10004273773322925 0.5189387904525777 8 0.06885500141903735 0.40818590663049653 9 0.05179716602423733 0.34544668987152155 >10 0.23214253156389644 3.0987106789400705 >50 0.020036770754969804 1.0401682757517283 >100 0.01595570114252551 2.3389891242693146 >500 0.0023307236483100535 1.1864796438452336 >1k 0.00152953739420347 2.2700335413950152 >5k 7.283511400968905E-5 0.3281534019278084 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6080 0.32436198584223946 No Hit GTCCTACAGTGGACATTTCTAAATT 4038 0.21542330572877685 No Hit TATCAACGCAGAGTACTTTTTTTTT 3893 0.20768769915852606 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3800 0.2027262411513997 No Hit CTGTAGGACGTGGAATATGGCAAGA 3753 0.20021883764242185 No Hit GGTATCAACGCAGAGTACTTTTTTT 3656 0.19504398359197825 No Hit CTTTAGGACGTGAAATATGGCGAGG 3337 0.17802564913742652 No Hit CTGAAGGACCTGGAATATGGCGAGA 1906 0.10168321464067574 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 5.334901082931571E-5 0.0 0.0 0.0 6 0.0 5.334901082931571E-5 0.0 0.0 0.0 7 0.0 5.334901082931571E-5 0.0 0.0 0.0 8 0.0 5.334901082931571E-5 0.0 0.0 0.0 9 0.0 5.334901082931571E-5 0.0 1.0669802165863142E-4 0.0 10 0.0 5.334901082931571E-5 0.0 1.6004703248794711E-4 0.0 11 0.0 5.334901082931571E-5 0.0 1.6004703248794711E-4 0.0 12 0.0 5.334901082931571E-5 0.0 1.6004703248794711E-4 0.0 13 0.0 1.0669802165863142E-4 0.0 1.6004703248794711E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTACACG 35 0.0022370648 16.212137 4 CCGTTTG 40 0.005440622 14.18562 9 TCCAACG 270 0.0 14.010488 18 CGGTAGG 80 1.2851069E-6 13.586629 1 CCAACGT 140 3.6379788E-12 12.834608 19 GCGCCAC 90 5.7120633E-7 12.60944 13 TAGGACC 555 0.0 12.609439 4 GGTATCA 1170 0.0 12.330366 1 GGCGAGG 875 0.0 12.105062 19 GTCCTAT 190 0.0 11.961433 1 GCACCGT 65 8.335398E-4 11.6394825 6 CGTTAGG 60 0.004382821 11.528048 1 CCGTAGG 60 0.004382821 11.528048 1 CGTAGGA 60 0.004382821 11.528048 2 ATTTAGA 695 0.0 11.374024 1 CCAACGA 195 0.0 11.154504 19 TGTCCAC 740 0.0 11.118459 10 TAGAAAT 835 0.0 10.986069 4 GAAATGT 810 0.0 10.974882 6 ACACCGT 95 1.43050165E-5 10.950303 6 >>END_MODULE