FastQCFastQC Report
Thu 26 May 2016
SRR1511771_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511771_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1874449
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67380.3594656349679292No Hit
GTCCTACAGTGGACATTTCTAAATT45020.2401772467535793No Hit
TATCAACGCAGAGTACTTTTTTTTT44980.23996385071026208No Hit
CTGTAGGACGTGGAATATGGCAAGA42290.22561296679717613No Hit
GGTATCAACGCAGAGTACTTTTTTT40510.21611684286955793No Hit
GTCCTAAAGTGTGTATTTCTCATTT39550.21099533782994365No Hit
CTTTAGGACGTGAAATATGGCGAGG36980.19728464204680946No Hit
CTGAAGGACCTGGAATATGGCGAGA21540.11491376932634603No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21100.11256641284985613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1300.014.61154719
TCCAACG2150.014.13544618
GTCTAGG1352.5465852E-1112.7176211
GGCGAGG11100.011.97883619
GGCGTGC1201.006083E-811.8715668
ATGTCGA1201.006083E-811.87156617
GGTATCA13200.011.7059931
GCGCCAC907.4868112E-611.60775313
TGTCGAG1157.104245E-811.56187218
TTAGGAC16100.011.0932143
ACCTTTT5800.010.97096415
CCACCTT5900.010.94598713
GTCGAGG1053.477775E-610.85429319
GACGTGA13150.010.8333687
AGGACGT24650.010.829665
AATGTCC6000.010.7635538
GGACGTG24400.010.7038716
GTCCTAT2100.010.4466181
AGGCGTG1553.6798156E-910.4163427
TAGGACC5300.010.3962714
Produced by FastQC (version 0.11.5)