##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511771_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1874449 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.183951657260348 31.0 31.0 34.0 28.0 34.0 2 31.3724326455401 33.0 31.0 34.0 28.0 34.0 3 31.48180772056215 34.0 31.0 34.0 28.0 34.0 4 35.02971486554182 37.0 35.0 37.0 32.0 37.0 5 34.693700388754245 37.0 35.0 37.0 32.0 37.0 6 34.65568815155814 37.0 35.0 37.0 32.0 37.0 7 34.49765344375867 37.0 35.0 37.0 31.0 37.0 8 34.41376959309109 37.0 35.0 37.0 30.0 37.0 9 35.958344558854364 39.0 37.0 39.0 31.0 39.0 10 35.77074382925329 39.0 35.0 39.0 30.0 39.0 11 35.76643749709915 39.0 35.0 39.0 30.0 39.0 12 35.6196098160046 39.0 35.0 39.0 30.0 39.0 13 35.6047179731217 39.0 35.0 39.0 30.0 39.0 14 36.66870904463125 40.0 36.0 41.0 30.0 41.0 15 36.60533522117699 40.0 36.0 41.0 30.0 41.0 16 36.455188698118754 40.0 36.0 41.0 30.0 41.0 17 36.437504568009054 40.0 36.0 41.0 30.0 41.0 18 36.37660933959793 40.0 36.0 41.0 30.0 41.0 19 36.40110667188064 40.0 36.0 41.0 30.0 41.0 20 36.50686895188933 40.0 37.0 41.0 30.0 41.0 21 36.45485473331096 40.0 37.0 41.0 30.0 41.0 22 36.361848735281676 40.0 36.0 41.0 30.0 41.0 23 36.261947911092804 40.0 36.0 41.0 30.0 41.0 24 36.12452619409757 40.0 36.0 41.0 29.0 41.0 25 35.635169588503075 39.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31952119724208217 1101 2 0.38652229112339853 1101 3 0.3035603428605569 1101 4 0.3220650147433659 1101 5 0.5021456313859147 1101 6 0.2846001166137029 1101 7 0.2940732752991835 1101 8 0.2015590170590258 1101 9 0.21991762273378157 1101 10 0.38628924541301046 1101 11 0.23340636006098947 1101 12 0.3715700773109347 1101 13 0.3796117612622396 1101 14 0.2795537144943623 1101 15 0.2810553886850684 1101 16 0.0711882250046969 1101 17 0.0019602206331725824 1101 18 0.17809203415340136 1101 19 0.21482785907659263 1101 20 -0.1161425480959295 1101 21 0.046293926596177926 1101 22 0.09140325591649656 1101 23 -0.06511388639543014 1101 24 -0.21099007083633126 1101 25 -0.11376035289923436 1102 1 0.41573874586949344 1102 2 0.40962041802366755 1102 3 0.5031346998328381 1102 4 0.5097527176568661 1102 5 0.5678545378627504 1102 6 0.6993248978769628 1102 7 0.7164489836479362 1102 8 0.5698978346553147 1102 9 0.6825790028751726 1102 10 0.8406164894398955 1102 11 0.8293856094352421 1102 12 0.80280510966724 1102 13 0.9319763320785199 1102 14 0.9916637107255184 1102 15 0.8441134272287059 1102 16 0.9369780011217088 1102 17 1.126105681201409 1102 18 1.1463200679479542 1102 19 1.2807116873456437 1102 20 1.2272703366263897 1102 21 0.9342953901930073 1102 22 1.149195472488266 1102 23 1.1167160490609618 1102 24 0.9336347599458463 1102 25 1.2360942169537097 1103 1 0.40665881167744544 1103 2 0.42239004216779463 1103 3 0.5158725759553171 1103 4 0.591317862562164 1103 5 0.7594729787418331 1103 6 0.708200098031952 1103 7 0.6151219163429431 1103 8 0.6548209433890335 1103 9 0.6339670833653628 1103 10 0.8844816726870377 1103 11 0.575449058263537 1103 12 0.7055877830623132 1103 13 0.8458850589584301 1103 14 0.7927102557674104 1103 15 0.7860226881266144 1103 16 0.8355011482786736 1103 17 1.1205416368941954 1103 18 0.8318949879877167 1103 19 0.969970191948704 1103 20 0.9417843924524334 1103 21 0.9996014777540552 1103 22 0.9488638277697916 1103 23 1.0371388187420862 1103 24 0.954120374428804 1103 25 1.1559288341824683 1104 1 0.27497730479698035 1104 2 0.33088019063056606 1104 3 0.28830797782345385 1104 4 0.4004983310824173 1104 5 0.30760362719399126 1104 6 0.38674045158981585 1104 7 0.4455395038911121 1104 8 0.5114355253012519 1104 9 0.6035256969043701 1104 10 0.5674293726812749 1104 11 0.49833300142505976 1104 12 0.4271177651449136 1104 13 0.5488086871860673 1104 14 0.6612121672597127 1104 15 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-0.7904165775830094 2314 24 -0.7816809348207983 2314 25 -0.9797380431879503 2315 1 -0.6529132186051925 2315 2 -0.6142383414094752 2315 3 -0.7044768987528975 2315 4 -0.5947681423060374 2315 5 -0.7555488359101545 2315 6 -0.9009517826824691 2315 7 -0.9466750826486106 2315 8 -0.9448382616393474 2315 9 -1.0072274915896102 2315 10 -1.2505789072619322 2315 11 -1.0321262132927131 2315 12 -1.1047164689603335 2315 13 -1.0110628185943895 2315 14 -1.2014141495972481 2315 15 -1.2224604964561578 2315 16 -1.171727772435915 2315 17 -1.2827579209424158 2315 18 -1.3305654657486627 2315 19 -1.2207240402195794 2315 20 -1.273740385679659 2315 21 -1.2591636934401222 2315 22 -1.1970658160465248 2315 23 -1.2243733632891818 2315 24 -1.179653969192323 2315 25 -1.473000895592591 2316 1 -1.1271521394526935 2316 2 -1.1370009213143533 2316 3 -1.0902141742494926 2316 4 -1.0718360849127748 2316 5 -1.2815969414690827 2316 6 -1.2767506644983868 2316 7 -1.4580265679392994 2316 8 -2.1091337462247424 2316 9 -1.7457095343226499 2316 10 -1.6430158031957447 2316 11 -1.4938981029383243 2316 12 -1.7040895525289343 2316 13 -1.9667786013399393 2316 14 -1.8389397047988254 2316 15 -1.969801620076879 2316 16 -2.0868784921892782 2316 17 -1.9130037918696914 2316 18 -1.8850108185256502 2316 19 -2.0473888100741604 2316 20 -2.0192897231646114 2316 21 -1.9606004618066422 2316 22 -1.988526354674363 2316 23 -2.0470429726861425 2316 24 -2.2179523751354893 2316 25 -2.1591329284074448 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23762.0 3 0.0 4 402.0 5 3937.0 6 5161.0 7 4075.0 8 3358.0 9 2968.0 10 2858.0 11 2756.0 12 2608.0 13 2527.0 14 2453.0 15 2463.0 16 2585.0 17 2573.0 18 2844.0 19 3152.0 20 3926.0 21 5051.0 22 6656.0 23 8489.0 24 10216.0 25 12105.0 26 14372.0 27 16182.0 28 18931.0 29 23298.0 30 29590.0 31 38457.0 32 49502.0 33 65009.0 34 86757.0 35 117168.0 36 174390.0 37 319035.0 38 804833.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.33729368481453 20.041939436410697 13.966560960856706 24.654205917918073 2 14.232662505088161 20.91996101254289 42.02434955552272 22.823026926846236 3 18.294869585675578 25.591147051746937 30.505497882311015 25.60848548026647 4 12.21446942541515 17.919132502404704 39.02330764934122 30.843090422838927 5 12.596374138990093 38.3546525621232 36.35397693584806 12.694996363038651 6 30.549030675147737 38.415769114016975 18.486926024661113 12.548274186174178 7 26.989371276572477 32.00321801233322 23.076274681252997 17.9311360298413 8 24.075768399140227 38.326142775823726 20.999024246591933 16.59906457844412 9 26.092841149585826 15.513145463013398 20.80878167397459 37.58523171342619 10 14.583112157225935 29.77920444888071 35.04955322870881 20.58813016518454 11 33.73812784450257 22.687680486372262 25.39978414990218 18.17440751922298 12 22.888006022036343 24.931913324929088 32.742261859351736 19.437818793682837 13 28.77362894375894 22.431018395272424 26.328537079429744 22.466815581538896 14 20.20487087138674 22.85386265510558 28.459616666017588 28.481649807490093 15 22.261101795781055 31.920473696536956 24.014470385697344 21.80395412198465 16 21.332668960318472 28.604832673494982 28.76621343125366 21.29628493493288 17 20.87370742015387 29.038453433515667 28.74300661154291 21.344832534787557 18 20.758633603795033 27.814413729047843 31.06326179053151 20.36369087662561 19 22.880643858541898 27.1577407547498 28.381407016141807 21.5802083705665 20 22.841432335582347 27.864721846259886 29.0791053797676 20.214740438390162 21 22.398422149655712 27.148458026865498 28.02839661148423 22.424723211994564 22 22.44158149941663 28.569195534261 28.389249320733718 20.59997364558865 23 21.6129646632157 28.253902880259744 28.726468418185824 21.406664038338732 24 22.419975150030755 27.24523313250987 29.457830007644915 20.876961709814456 25 22.12646452984864 27.806660986648048 29.03947755210048 21.02739693140284 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 265.0 1 265.0 2 490.5 3 716.0 4 716.0 5 716.0 6 1767.5 7 2819.0 8 2819.0 9 2819.0 10 3956.5 11 5094.0 12 5094.0 13 5094.0 14 7664.0 15 10234.0 16 10234.0 17 10234.0 18 18090.0 19 25946.0 20 25946.0 21 25946.0 22 41148.5 23 56351.0 24 56351.0 25 56351.0 26 80316.0 27 104281.0 28 104281.0 29 104281.0 30 133967.0 31 163653.0 32 163653.0 33 163653.0 34 180350.0 35 197047.0 36 197047.0 37 197047.0 38 207201.5 39 217356.0 40 217356.0 41 217356.0 42 226868.0 43 236380.0 44 236380.0 45 236380.0 46 238624.5 47 240869.0 48 240869.0 49 240869.0 50 227921.5 51 214974.0 52 214974.0 53 214974.0 54 188088.0 55 161202.0 56 161202.0 57 161202.0 58 136205.0 59 111208.0 60 111208.0 61 111208.0 62 89675.5 63 68143.0 64 68143.0 65 68143.0 66 51306.0 67 34469.0 68 34469.0 69 34469.0 70 24940.5 71 15412.0 72 15412.0 73 15412.0 74 10468.0 75 5524.0 76 5524.0 77 5524.0 78 3658.5 79 1793.0 80 1793.0 81 1793.0 82 1176.5 83 560.0 84 560.0 85 560.0 86 340.5 87 121.0 88 121.0 89 121.0 90 73.5 91 26.0 92 26.0 93 26.0 94 15.0 95 4.0 96 4.0 97 4.0 98 3.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42401793807140126 2 0.0 3 0.0 4 0.0 5 0.033716574844127524 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.00224065845483126 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1874449.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.87591138000985 #Duplication Level Percentage of deduplicated Percentage of total 1 83.80557093583013 60.23601789734857 2 11.57618550524617 16.64097766987257 3 2.653071194951519 5.720757302795768 4 0.8590647305915081 2.469842417827491 5 0.3630946172694685 1.304887826670946 6 0.18895265766807226 0.814868668654062 7 0.11464997826671139 0.5768400174332743 8 0.07357962772163984 0.4230882241195762 9 0.05695552083567884 0.3684356971368802 >10 0.2655030811864676 3.4133093028982584 >50 0.020898893092747044 1.0820249562226918 >100 0.017875900418981582 2.537799636856206 >500 0.0026694330508820296 1.3223431376636217 >1k 0.0018537729520014042 2.7296944291530996 >5k 7.415091808005618E-5 0.35911281534712275 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6738 0.3594656349679292 No Hit GTCCTACAGTGGACATTTCTAAATT 4502 0.2401772467535793 No Hit TATCAACGCAGAGTACTTTTTTTTT 4498 0.23996385071026208 No Hit CTGTAGGACGTGGAATATGGCAAGA 4229 0.22561296679717613 No Hit GGTATCAACGCAGAGTACTTTTTTT 4051 0.21611684286955793 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3955 0.21099533782994365 No Hit CTTTAGGACGTGAAATATGGCGAGG 3698 0.19728464204680946 No Hit CTGAAGGACCTGGAATATGGCGAGA 2154 0.11491376932634603 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2110 0.11256641284985613 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 5.334901082931571E-5 0.0 7 0.0 0.0 0.0 5.334901082931571E-5 0.0 8 0.0 0.0 0.0 5.334901082931571E-5 0.0 9 0.0 0.0 0.0 1.6004703248794711E-4 0.0 10 5.334901082931571E-5 0.0 0.0 2.1339604331726284E-4 0.0 11 5.334901082931571E-5 0.0 0.0 2.1339604331726284E-4 0.0 12 5.334901082931571E-5 0.0 0.0 2.1339604331726284E-4 0.0 13 5.334901082931571E-5 0.0 0.0 2.1339604331726284E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 130 0.0 14.611547 19 TCCAACG 215 0.0 14.135446 18 GTCTAGG 135 2.5465852E-11 12.717621 1 GGCGAGG 1110 0.0 11.978836 19 GGCGTGC 120 1.006083E-8 11.871566 8 ATGTCGA 120 1.006083E-8 11.871566 17 GGTATCA 1320 0.0 11.705993 1 GCGCCAC 90 7.4868112E-6 11.607753 13 TGTCGAG 115 7.104245E-8 11.561872 18 TTAGGAC 1610 0.0 11.093214 3 ACCTTTT 580 0.0 10.970964 15 CCACCTT 590 0.0 10.945987 13 GTCGAGG 105 3.477775E-6 10.854293 19 GACGTGA 1315 0.0 10.833368 7 AGGACGT 2465 0.0 10.82966 5 AATGTCC 600 0.0 10.763553 8 GGACGTG 2440 0.0 10.703871 6 GTCCTAT 210 0.0 10.446618 1 AGGCGTG 155 3.6798156E-9 10.416342 7 TAGGACC 530 0.0 10.396271 4 >>END_MODULE