FastQCFastQC Report
Thu 26 May 2016
SRR1511770_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511770_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2299848
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49190.2138837001401832No Hit
GTCCTACAGTGGACATTTCTAAATT36420.15835829150448202No Hit
CTGTAGGACGTGGAATATGGCAAGA33790.14692275315586073No Hit
GTCCTAAAGTGTGTATTTCTCATTT32340.14061798866707714No Hit
TATCAACGCAGAGTACTTTTTTTTT30390.13213916745802332No Hit
GGTATCAACGCAGAGTACTTTTTTT29760.12939985599048284No Hit
CTTTAGGACGTGAAATATGGCGAGG28650.12457345007148299No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACT400.005442641714.1850214
AGGACCG957.847666E-812.94072155
GGCGAGG8450.012.19882419
TCCAACG3100.011.89737318
ACACCGT658.339607E-411.6389928
CCAACGA2050.011.53278819
GGTATCA10000.011.2681271
GGACCGA700.00154983810.8076356
TAGGACC6050.010.7853054
CCAACGT1252.3862776E-710.59171319
TTAGGAC13050.010.5074233
TGTAGAA11750.010.4311142
AGGACGT19650.010.395135
GTATCAA20850.010.3346881
GGACGTG20100.010.11535556
GACGTGA10600.010.0811797
GACCGAG856.8784493E-410.0129577
GGACCGC856.8784493E-410.0129576
TAGGACG21400.09.9869624
GGCCTAA1104.4277513E-59.8843221
Produced by FastQC (version 0.11.5)