FastQCFastQC Report
Thu 26 May 2016
SRR1511770_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511770_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2299848
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66210.2878885908981811No Hit
TATCAACGCAGAGTACTTTTTTTTT41170.17901183034704904No Hit
GTCCTACAGTGGACATTTCTAAATT40660.1767942924923734No Hit
GGTATCAACGCAGAGTACTTTTTTT39000.16957642418107632No Hit
CTGTAGGACGTGGAATATGGCAAGA35690.15518416869288754No Hit
GTCCTAAAGTGTGTATTTCTCATTT33250.1445747718979689No Hit
CTTTAGGACGTGAAATATGGCGAGG30560.13287834674291518No Hit
TTGTAGAACAGTGTATATCAATGAG24250.10544175093310515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10250.013.4898221
CCAACGA2600.013.15074519
TAGGACC6200.012.4116024
TCCAACG3950.012.26239818
CGTGCGC907.4880427E-611.6078710
GCGTGCG951.3650888E-510.9969289
ACTGCGC700.001495353410.8541128
CCGGTTT2450.010.85411212
CGGTTTC2400.010.68451613
TTAGGAC12850.010.6461833
GCCGGTT2500.010.6370311
AGGACGT21100.010.5808355
GGCGAGG9550.010.54201719
TGTAGGA22950.010.4730312
GGACGTG20650.010.4401846
GTAGGAC21600.010.2919253
GTATCAA23050.010.2598931
TAGGACG22400.010.1788274
GACGTGA10950.010.147857
CCGTGTA750.002654538710.1305059
Produced by FastQC (version 0.11.5)