##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511770_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2299848 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.95716369081783 31.0 31.0 34.0 28.0 34.0 2 31.145223075611952 31.0 31.0 34.0 28.0 34.0 3 31.252815838264095 33.0 31.0 34.0 28.0 34.0 4 34.810640529287156 37.0 35.0 37.0 32.0 37.0 5 34.44884314093801 37.0 35.0 37.0 30.0 37.0 6 34.40008122275907 37.0 35.0 37.0 30.0 37.0 7 34.22716022971953 37.0 35.0 37.0 30.0 37.0 8 34.140028819295885 37.0 35.0 37.0 30.0 37.0 9 35.648369805308874 39.0 35.0 39.0 30.0 39.0 10 35.45035845847204 39.0 35.0 39.0 30.0 39.0 11 35.450067569682865 39.0 35.0 39.0 30.0 39.0 12 35.27520775286019 39.0 35.0 39.0 29.0 39.0 13 35.26246430198865 39.0 35.0 39.0 29.0 39.0 14 36.28941477871581 40.0 36.0 41.0 29.0 41.0 15 36.214360253373265 40.0 36.0 41.0 29.0 41.0 16 36.053202646435764 39.0 36.0 41.0 27.0 41.0 17 36.01977695917295 39.0 36.0 41.0 27.0 41.0 18 35.94408108709793 39.0 36.0 41.0 27.0 41.0 19 35.95973081699312 39.0 36.0 41.0 27.0 41.0 20 36.0684984398969 40.0 36.0 41.0 28.0 41.0 21 36.00784747513749 40.0 36.0 41.0 28.0 41.0 22 35.91210766972426 40.0 36.0 41.0 27.0 41.0 23 35.80425141139762 39.0 36.0 41.0 27.0 41.0 24 35.6508869281796 39.0 36.0 41.0 27.0 41.0 25 35.126838817174004 39.0 35.0 40.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3592512494362481 1101 2 0.38745107785864263 1101 3 0.4422969620613131 1101 4 0.35087550840652426 1101 5 0.5055759684030576 1101 6 0.43323811969226966 1101 7 0.4408395908760525 1101 8 0.3852228068399768 1101 9 0.5780195904984282 1101 10 0.5714595386884795 1101 11 0.6026163746362485 1101 12 0.7596889569399679 1101 13 0.5362831199121771 1101 14 0.5219228749176921 1101 15 0.7010742634425284 1101 16 0.48714319087250857 1101 17 0.43375636979416043 1101 18 0.5920963713883793 1101 19 0.43882326618137313 1101 20 0.13896239518454934 1101 21 0.5781586734486126 1101 22 0.39341379266132037 1101 23 0.5032118520491267 1101 24 0.21268411805025522 1101 25 0.47312108121361973 1102 1 0.3709220088776384 1102 2 0.3882635837715682 1102 3 0.31784128416207835 1102 4 0.3241806484137726 1102 5 0.5114585305695627 1102 6 0.5907154606251197 1102 7 0.6546835011829444 1102 8 0.5277331500789657 1102 9 0.549901272409862 1102 10 0.7761846549690077 1102 11 0.7385015766001004 1102 12 0.8468298531596332 1102 13 0.7141217428816091 1102 14 0.8764159624474246 1102 15 0.8617423475182591 1102 16 0.7969922481793361 1102 17 0.9694085146718479 1102 18 1.0269997187530464 1102 19 1.1232218218662169 1102 20 0.8808054576591857 1102 21 0.7558709926400411 1102 22 0.8099590552655016 1102 23 0.8787079028784106 1102 24 0.781916614512177 1102 25 1.0958071754338192 1103 1 0.40716140312264315 1103 2 0.500431207331431 1103 3 0.4978149293660614 1103 4 0.4655423955264695 1103 5 0.570019392767648 1103 6 0.5970233024763161 1103 7 0.5410305295211515 1103 8 0.5660699130643749 1103 9 0.5091576732945882 1103 10 0.6651318769508237 1103 11 0.4932722739007005 1103 12 0.6548873540125797 1103 13 0.6887106078253211 1103 14 0.8952307691030228 1103 15 0.7869458914682284 1103 16 0.941543082966426 1103 17 1.0694043900294474 1103 18 1.0147662201240735 1103 19 0.91907348065196 1103 20 1.005736822894363 1103 21 1.1713629110827455 1103 22 1.1273963028844776 1103 23 1.094671318015564 1103 24 0.9644544521457448 1103 25 1.0574680282620506 1104 1 0.3077949849852928 1104 2 0.4232210650538022 1104 3 0.45626021128791194 1104 4 0.4966044202193203 1104 5 0.5028205706944888 1104 6 0.6365113127652364 1104 7 0.7374779356487764 1104 8 0.8018618144953393 1104 9 0.7590736112317131 1104 10 0.8866852058775123 1104 11 0.96903572072587 1104 12 0.9463200613537808 1104 13 0.7986985148478496 1104 14 0.9542588966602423 1104 15 1.1210860366115583 1104 16 1.1593507627660955 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-0.7479889301679989 2315 3 -0.7764725305456324 2315 4 -0.7885443756849639 2315 5 -0.9098727670028666 2315 6 -0.9560109593940282 2315 7 -0.9787488102032995 2315 8 -1.025120624028716 2315 9 -1.0306084113298652 2315 10 -1.371269954491801 2315 11 -1.2030991032435097 2315 12 -1.2010829595850936 2315 13 -1.3806793698753523 2315 14 -1.3581754620493953 2315 15 -1.3544008664462481 2315 16 -1.4804524320601118 2315 17 -1.5138989964027445 2315 18 -1.392071716662592 2315 19 -1.5158570033056549 2315 20 -1.5328700943428615 2315 21 -1.6053488093235728 2315 22 -1.5507861896078765 2315 23 -1.5245081944331957 2315 24 -1.409033360119352 2315 25 -1.5320596057967535 2316 1 -0.9777611364881338 2316 2 -1.0013920755591137 2316 3 -1.0399132438550147 2316 4 -1.0151618583329736 2316 5 -1.0645718997400664 2316 6 -1.2436689649782267 2316 7 -1.3818883002622613 2316 8 -2.064475792551974 2316 9 -1.6962248183180009 2316 10 -1.7027724210690494 2316 11 -1.437518679868404 2316 12 -1.586949930540996 2316 13 -1.6413096411379158 2316 14 -1.554636881009614 2316 15 -1.7221640391105097 2316 16 -1.8837475303992903 2316 17 -1.7559123340720717 2316 18 -1.7789305781564693 2316 19 -1.7017986354479433 2316 20 -1.7902065501045072 2316 21 -1.7218506633455561 2316 22 -1.9073581379120554 2316 23 -1.8193765207077135 2316 24 -1.9691100225334068 2316 25 -2.106167953032852 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35408.0 3 0.0 4 630.0 5 5691.0 6 7159.0 7 5679.0 8 4734.0 9 4055.0 10 3849.0 11 3806.0 12 3495.0 13 3504.0 14 3465.0 15 3418.0 16 3437.0 17 3610.0 18 4028.0 19 4404.0 20 5387.0 21 6925.0 22 8974.0 23 11284.0 24 13955.0 25 16339.0 26 19054.0 27 21839.0 28 25309.0 29 30947.0 30 39216.0 31 50760.0 32 65382.0 33 84752.0 34 112227.0 35 151219.0 36 225531.0 37 406888.0 38 903488.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.344715849721794 19.90949179081635 14.12400515418227 24.62178720527959 2 14.652316153067508 20.97860380338179 41.244986625203055 23.12409341834765 3 18.553878343264422 25.28893213812391 30.403313610290766 25.7538759083209 4 12.50756571738654 17.909314006838713 38.63081386248135 30.9523064132934 5 12.976920273118656 37.88899356871506 36.20022669996703 12.933859458199256 6 31.01135379381594 38.066646143571226 18.273642432021596 12.648357630591239 7 27.23453897822813 31.810450081918457 22.814290335709142 18.140720604144274 8 24.390481457905043 37.89576528535799 20.854682570326386 16.85907068641058 9 25.937105408705268 15.659034857955831 20.55892389410083 37.84493583923807 10 14.472086850957105 29.817318361909134 34.797517053300915 20.913077733832843 11 34.24052372156768 22.657410402774445 24.862382209606896 18.239683666050972 12 22.76189556875063 24.815161697642626 32.49101679763184 19.9319259359749 13 28.66767716823025 22.098329976589756 26.32265262747799 22.911340227702006 14 20.575142357234043 22.388088256267373 28.108553260911158 28.92821612558743 15 22.627191014362687 31.156580782729986 23.839662447257385 22.37656575564994 16 21.911013249571276 28.53727724614844 28.027765313185917 21.52394419109437 17 21.37606485298159 28.612151759594546 28.27239017535072 21.739393212073146 18 21.079958327680785 28.035331030572458 30.14668795503007 20.738022686716686 19 23.0215214222853 27.202015089692882 27.919801656457295 21.85666183156452 20 23.005737770496136 27.802924367175567 28.51610193369301 20.67523592863528 21 22.733676312521524 27.081441903986697 27.686873219447545 22.498008564044234 22 22.543968123110744 28.16329600912756 28.08685617484286 21.205879692918835 23 22.30977873320324 27.696960842629597 28.082334136864695 21.910926287302466 24 22.60162410733231 27.181665918791154 28.77246670214727 21.444243271729263 25 22.675803712939633 27.42774899501912 28.42518572307532 21.47126156896593 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 194.0 1 194.0 2 413.0 3 632.0 4 632.0 5 632.0 6 1518.0 7 2404.0 8 2404.0 9 2404.0 10 3670.5 11 4937.0 12 4937.0 13 4937.0 14 8235.5 15 11534.0 16 11534.0 17 11534.0 18 20605.0 19 29676.0 20 29676.0 21 29676.0 22 47616.0 23 65556.0 24 65556.0 25 65556.0 26 93046.5 27 120537.0 28 120537.0 29 120537.0 30 155732.0 31 190927.0 32 190927.0 33 190927.0 34 211313.5 35 231700.0 36 231700.0 37 231700.0 38 247293.5 39 262887.0 40 262887.0 41 262887.0 42 275458.5 43 288030.0 44 288030.0 45 288030.0 46 291117.0 47 294204.0 48 294204.0 49 294204.0 50 281823.0 51 269442.0 52 269442.0 53 269442.0 54 238719.0 55 207996.0 56 207996.0 57 207996.0 58 177148.5 59 146301.0 60 146301.0 61 146301.0 62 118654.5 63 91008.0 64 91008.0 65 91008.0 66 69717.5 67 48427.0 68 48427.0 69 48427.0 70 35014.0 71 21601.0 72 21601.0 73 21601.0 74 14791.0 75 7981.0 76 7981.0 77 7981.0 78 5387.5 79 2794.0 80 2794.0 81 2794.0 82 1801.5 83 809.0 84 809.0 85 809.0 86 512.5 87 216.0 88 216.0 89 216.0 90 130.5 91 45.0 92 45.0 93 45.0 94 27.0 95 9.0 96 9.0 97 9.0 98 5.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4204190885658531 2 0.0 3 0.0 4 0.0 5 0.03365439802978284 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0023045001234864217 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2299848.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.97630985044398 #Duplication Level Percentage of deduplicated Percentage of total 1 84.57358183899727 61.71867913444547 2 10.824000809314226 15.797912737639427 3 2.523512677991328 5.524699293018565 4 0.8417859504483385 2.4572172939067376 5 0.3883312816753363 1.416949196807969 6 0.21573243440669362 0.9446014186832075 7 0.1256039478705879 0.6416278832770126 8 0.08885123821814941 0.5187228392642627 9 0.06721373234027612 0.4414509141721918 >10 0.3134506334507524 3.886292048269613 >50 0.019770874552912467 1.0061276733481124 >100 0.014535515634946823 2.10829504039848 >500 0.0020227575962748832 1.0318609775369925 >1k 0.0015468146324442442 2.2181081896045782 >5k 5.949287047862478E-5 0.28745535962755747 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6621 0.2878885908981811 No Hit TATCAACGCAGAGTACTTTTTTTTT 4117 0.17901183034704904 No Hit GTCCTACAGTGGACATTTCTAAATT 4066 0.1767942924923734 No Hit GGTATCAACGCAGAGTACTTTTTTT 3900 0.16957642418107632 No Hit CTGTAGGACGTGGAATATGGCAAGA 3569 0.15518416869288754 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3325 0.1445747718979689 No Hit CTTTAGGACGTGAAATATGGCGAGG 3056 0.13287834674291518 No Hit TTGTAGAACAGTGTATATCAATGAG 2425 0.10544175093310515 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.3481134405404184E-5 2 0.0 0.0 0.0 0.0 4.3481134405404184E-5 3 0.0 0.0 0.0 0.0 4.3481134405404184E-5 4 0.0 0.0 0.0 0.0 4.3481134405404184E-5 5 0.0 0.0 0.0 4.3481134405404184E-5 4.3481134405404184E-5 6 0.0 0.0 0.0 4.3481134405404184E-5 8.696226881080837E-5 7 0.0 0.0 0.0 4.3481134405404184E-5 8.696226881080837E-5 8 0.0 0.0 0.0 4.3481134405404184E-5 8.696226881080837E-5 9 0.0 0.0 0.0 4.3481134405404184E-5 8.696226881080837E-5 10 0.0 0.0 0.0 4.3481134405404184E-5 8.696226881080837E-5 11 0.0 0.0 0.0 8.696226881080837E-5 8.696226881080837E-5 12 0.0 0.0 0.0 1.3044340321621256E-4 8.696226881080837E-5 13 0.0 0.0 0.0 1.3044340321621256E-4 8.696226881080837E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1025 0.0 13.489822 1 CCAACGA 260 0.0 13.150745 19 TAGGACC 620 0.0 12.411602 4 TCCAACG 395 0.0 12.262398 18 CGTGCGC 90 7.4880427E-6 11.60787 10 GCGTGCG 95 1.3650888E-5 10.996928 9 ACTGCGC 70 0.0014953534 10.854112 8 CCGGTTT 245 0.0 10.854112 12 CGGTTTC 240 0.0 10.684516 13 TTAGGAC 1285 0.0 10.646183 3 GCCGGTT 250 0.0 10.63703 11 AGGACGT 2110 0.0 10.580835 5 GGCGAGG 955 0.0 10.542017 19 TGTAGGA 2295 0.0 10.473031 2 GGACGTG 2065 0.0 10.440184 6 GTAGGAC 2160 0.0 10.291925 3 GTATCAA 2305 0.0 10.259893 1 TAGGACG 2240 0.0 10.178827 4 GACGTGA 1095 0.0 10.14785 7 CCGTGTA 75 0.0026545387 10.130505 9 >>END_MODULE