FastQCFastQC Report
Thu 26 May 2016
SRR1511769_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511769_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2280026
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52810.23162016573495214No Hit
TATCAACGCAGAGTACTTTTTTTTT32120.14087558650646967No Hit
GGTATCAACGCAGAGTACTTTTTTT31750.13925279799440882No Hit
GTCCTACAGTGGACATTTCTAAATT31290.13723527714157643No Hit
CTGTAGGACGTGGAATATGGCAAGA28030.12293719457585134No Hit
GTCCTAAAGTGTGTATTTCTCATTT26810.11758637840094807No Hit
CTTTAGGACGTGAAATATGGCGAGG23870.10469178860241068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2450.012.35182118
GGCGAGG8100.012.14214419
GTCCTAA8800.011.1203771
GTACTAG1457.774361E-910.9073421
TAGGACC5500.010.832174
TAATACT2550.010.7546284
GGTATCA12850.010.6924851
TTAATAC2300.010.6901333
AATCCCG4250.010.68069319
GTAGGAC18150.010.5247633
GTACTAA1707.8398443E-1010.4662371
AGGACGT19700.010.46472555
GGACGTG19550.010.3999026
TGGCGAG17250.010.3066118
GTATAAG2400.010.2966451
GTCCTAG4150.010.2420611
GACGTGA10350.010.2332897
CTAGAGT3450.010.1419214
TAGGACG20600.010.099344
AAACGGG750.002750252410.08732119
Produced by FastQC (version 0.11.5)