FastQCFastQC Report
Thu 26 May 2016
SRR1511769_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511769_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2280026
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65590.28767215812451263No Hit
TATCAACGCAGAGTACTTTTTTTTT43560.19105045293343145No Hit
GGTATCAACGCAGAGTACTTTTTTT40330.17688394781463018No Hit
GTCCTACAGTGGACATTTCTAAATT30920.1356124886295156No Hit
CTGTAGGACGTGGAATATGGCAAGA29210.1281125741548561No Hit
GTCCTAAAGTGTGTATTTCTCATTT27340.11991091329660276No Hit
CTTTAGGACGTGAAATATGGCGAGG25030.10977944988346625No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGAC400.005279700314.2501643
GGTATCA14800.011.9232631
TAGGACC7350.011.89129454
CCAACGA1950.011.68936519
GCCGGTT1657.2759576E-1211.51174911
CAAGGCG600.00588810411.08054419
TCCAACG3450.011.01123818
GTCCTAA8700.010.9639211
CCGGTTT1752.0008883E-1110.85393512
TGCCGGT1752.0008883E-1110.85393510
CTAGGAC3300.010.6516383
GTAGGAC19200.010.2917853
TGTAGGA20200.010.2525942
CTGTAGG19800.010.1167081
GGCGAGG6950.010.11256319
TACACCG856.5947586E-410.058945
AAGTGCG856.613402E-410.0558517
GGACGTG15800.010.0381736
AGGACGT16250.09.9970385
ACTGTTC8000.09.9720548
Produced by FastQC (version 0.11.5)