##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511769_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2280026 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.526994867602387 31.0 31.0 34.0 27.0 34.0 2 30.71849136808089 31.0 31.0 34.0 27.0 34.0 3 30.814763077263155 31.0 31.0 34.0 27.0 34.0 4 34.39634679604531 37.0 35.0 37.0 30.0 37.0 5 33.974721779488476 37.0 35.0 37.0 30.0 37.0 6 33.921018883118 37.0 35.0 37.0 30.0 37.0 7 33.72598733523214 37.0 35.0 37.0 29.0 37.0 8 33.61770348232871 37.0 35.0 37.0 28.0 37.0 9 35.061647542615745 38.0 35.0 39.0 28.0 39.0 10 34.83641063742255 38.0 35.0 39.0 28.0 39.0 11 34.831726918903556 38.0 35.0 39.0 27.0 39.0 12 34.623332804099604 38.0 35.0 39.0 27.0 39.0 13 34.608345255711995 38.0 35.0 39.0 27.0 39.0 14 35.53907016849808 39.0 36.0 41.0 27.0 41.0 15 35.44669183597029 39.0 35.0 41.0 27.0 41.0 16 35.27071270239901 39.0 35.0 40.0 27.0 41.0 17 35.22708030522459 39.0 35.0 40.0 27.0 41.0 18 35.13178709365595 39.0 35.0 40.0 26.0 41.0 19 35.14835795732154 39.0 35.0 40.0 26.0 41.0 20 35.2524357178383 39.0 35.0 40.0 26.0 41.0 21 35.18875398789312 39.0 35.0 41.0 26.0 41.0 22 35.0595918643033 39.0 35.0 41.0 25.0 41.0 23 34.93328102398832 39.0 35.0 40.0 25.0 41.0 24 34.747101129548525 39.0 34.0 41.0 24.0 41.0 25 34.18189792572541 38.0 34.0 40.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16036417240600898 1101 2 0.3195389938688393 1101 3 0.3766316734766839 1101 4 0.25056900601408216 1101 5 0.4510495769656302 1101 6 0.5191392704147546 1101 7 0.4149159347911109 1101 8 0.4080998324899596 1101 9 0.44280572921120864 1101 10 0.49661243811703315 1101 11 0.4399832977011897 1101 12 0.5709790191705295 1101 13 0.6160015904565697 1101 14 0.4630833746976464 1101 15 0.47559842220226756 1101 16 0.3410941442409907 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-1.226081526406567 2315 7 -1.1149332935813518 2315 8 -1.3042300777205895 2315 9 -1.2207917124431589 2315 10 -1.4369805071729758 2315 11 -1.1946628846172587 2315 12 -1.2873530941467948 2315 13 -1.2355015996524727 2315 14 -1.447195530275586 2315 15 -1.4540720319156293 2315 16 -1.320265732170455 2315 17 -1.542658995384258 2315 18 -1.486405188197466 2315 19 -1.468862755373891 2315 20 -1.3096898143741669 2315 21 -1.4522468041767667 2315 22 -1.4448641841284697 2315 23 -1.5251302122580128 2315 24 -1.5982198539144576 2315 25 -1.6569972540664182 2316 1 -1.1350150477028933 2316 2 -1.2049037986429916 2316 3 -1.2063326711127793 2316 4 -1.1762222478052777 2316 5 -1.1991148597174046 2316 6 -1.4166988224658255 2316 7 -1.4416769190788088 2316 8 -2.1430497194943783 2316 9 -1.6751951781813332 2316 10 -1.6972825255534048 2316 11 -1.7490056518226567 2316 12 -1.9068237215160906 2316 13 -2.070106425370888 2316 14 -1.921161627307491 2316 15 -2.125908406189943 2316 16 -1.9846142577173467 2316 17 -1.743196091039657 2316 18 -1.9411886366205238 2316 19 -2.0163477912424668 2316 20 -2.006057587031023 2316 21 -2.1491873267084713 2316 22 -2.2585810655686274 2316 23 -2.018947011872349 2316 24 -2.246116393758818 2316 25 -2.1350328977993414 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 47049.0 3 1.0 4 842.0 5 7177.0 6 8908.0 7 7022.0 8 5888.0 9 5011.0 10 4781.0 11 4726.0 12 4476.0 13 4191.0 14 4134.0 15 4028.0 16 4146.0 17 4409.0 18 4699.0 19 5283.0 20 6481.0 21 8266.0 22 10530.0 23 12982.0 24 15849.0 25 18923.0 26 21536.0 27 24638.0 28 28667.0 29 34307.0 30 43979.0 31 56234.0 32 72290.0 33 93179.0 34 123147.0 35 165546.0 36 246340.0 37 435331.0 38 735030.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.57367246018151 19.932978999793036 14.084626122337546 24.40872241768791 2 15.341272424086391 21.16888140749272 40.10691106154053 23.38293510688036 3 19.010090235813102 25.18541455229019 30.215137897550292 25.589357314346415 4 13.213577389029773 17.874357573115393 37.807551317397255 31.10451372045757 5 13.405439127886368 37.81320524924803 35.68017176932229 13.101183853543313 6 31.633191902197606 37.66680730833771 17.861682279061732 12.838318510402951 7 27.730166234946445 31.742752056336197 22.394349888992494 18.132731819724864 8 25.04725823302015 37.278697699061325 20.54494115417982 17.129102913738702 9 26.22864826980043 15.489954939110342 20.168892810871455 38.112503980217774 10 14.911847496475916 29.57080313996419 34.30250356794177 21.21484579561812 11 34.62675425631111 22.5054451133452 24.30981050216094 18.557990128182748 12 23.027149690398268 24.795638295352774 31.83529486067264 20.34191715357632 13 29.117474976162555 21.838347457441277 25.86347699543777 23.1807005709584 14 20.983576503074968 21.9843107052288 27.805428534586884 29.226684257109348 15 23.190349583732818 30.5605286957254 23.610213216866825 22.63890850367496 16 22.500927621000812 28.151345642549693 27.398722646145263 21.949004090304232 17 21.860408609375508 28.254984811576712 27.842270219725563 22.042336359322217 18 21.727208373939597 27.66442137063349 29.451681691349137 21.15668856407778 19 23.501442527409775 26.94631552447209 27.59937825270414 21.952863695414 20 23.414206680099262 27.29363612520208 28.12441612507928 21.16774106961938 21 23.253989208894986 26.703818289791432 27.348416202271373 22.693776299042202 22 23.109166298980803 27.672403735746876 27.634553290181778 21.583876675090547 23 22.82530988681708 27.287890576686408 27.583018790136606 22.303780746359912 24 23.060175629576154 26.86307085971827 28.214590535371087 21.862162975334492 25 22.996810932741866 27.184484755767276 27.91808864738548 21.900615664105384 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 238.0 1 238.0 2 441.5 3 645.0 4 645.0 5 645.0 6 1616.5 7 2588.0 8 2588.0 9 2588.0 10 3706.5 11 4825.0 12 4825.0 13 4825.0 14 7704.0 15 10583.0 16 10583.0 17 10583.0 18 18996.5 19 27410.0 20 27410.0 21 27410.0 22 43681.5 23 59953.0 24 59953.0 25 59953.0 26 85010.0 27 110067.0 28 110067.0 29 110067.0 30 141660.0 31 173253.0 32 173253.0 33 173253.0 34 194181.0 35 215109.0 36 215109.0 37 215109.0 38 232292.5 39 249476.0 40 249476.0 41 249476.0 42 265069.5 43 280663.0 44 280663.0 45 280663.0 46 287949.0 47 295235.0 48 295235.0 49 295235.0 50 286798.0 51 278361.0 52 278361.0 53 278361.0 54 248650.0 55 218939.0 56 218939.0 57 218939.0 58 188564.0 59 158189.0 60 158189.0 61 158189.0 62 129701.5 63 101214.0 64 101214.0 65 101214.0 66 77899.0 67 54584.0 68 54584.0 69 54584.0 70 39722.0 71 24860.0 72 24860.0 73 24860.0 74 17167.0 75 9474.0 76 9474.0 77 9474.0 78 6332.5 79 3191.0 80 3191.0 81 3191.0 82 2037.0 83 883.0 84 883.0 85 883.0 86 553.5 87 224.0 88 224.0 89 224.0 90 141.0 91 58.0 92 58.0 93 58.0 94 31.0 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3989428190731158 2 0.0 3 0.0 4 0.0 5 0.030262812792485702 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.002149098299756231 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2280026.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.29561788902619 #Duplication Level Percentage of deduplicated Percentage of total 1 81.23993943976133 57.10811740185091 2 12.650477242427236 17.785462286949734 3 3.34389926647266 7.051843952860713 4 1.2289964796253 3.4557226767479383 5 0.5334928397158608 1.8751104403598826 6 0.30428537272360584 1.2833956974119105 7 0.16690833888356296 0.8213047368855725 8 0.10976580223750146 0.6172843913095855 9 0.07670007691962429 0.48525113692807437 >10 0.30891800926393886 3.675342443856703 >50 0.01845945572682446 0.8741395646773812 >100 0.014916530638000452 2.0455244309225753 >500 0.0018075619718030584 0.8426513113820027 >1k 0.0013712539096398038 1.791466522899323 >5k 6.232972316544562E-5 0.28738300495748426 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6559 0.28767215812451263 No Hit TATCAACGCAGAGTACTTTTTTTTT 4356 0.19105045293343145 No Hit GGTATCAACGCAGAGTACTTTTTTT 4033 0.17688394781463018 No Hit GTCCTACAGTGGACATTTCTAAATT 3092 0.1356124886295156 No Hit CTGTAGGACGTGGAATATGGCAAGA 2921 0.1281125741548561 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2734 0.11991091329660276 No Hit CTTTAGGACGTGAAATATGGCGAGG 2503 0.10977944988346625 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.385914897461696E-5 0.0 0.0 0.0 6 0.0 4.385914897461696E-5 0.0 0.0 0.0 7 0.0 4.385914897461696E-5 0.0 4.385914897461696E-5 0.0 8 0.0 4.385914897461696E-5 0.0 4.385914897461696E-5 0.0 9 0.0 4.385914897461696E-5 0.0 8.771829794923392E-5 0.0 10 4.385914897461696E-5 4.385914897461696E-5 0.0 1.3157744692385086E-4 0.0 11 4.385914897461696E-5 4.385914897461696E-5 0.0 1.3157744692385086E-4 0.0 12 4.385914897461696E-5 4.385914897461696E-5 0.0 1.3157744692385086E-4 0.0 13 4.385914897461696E-5 4.385914897461696E-5 0.0 1.7543659589846784E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCACGAC 40 0.0052797003 14.250164 3 GGTATCA 1480 0.0 11.923263 1 TAGGACC 735 0.0 11.8912945 4 CCAACGA 195 0.0 11.689365 19 GCCGGTT 165 7.2759576E-12 11.511749 11 CAAGGCG 60 0.005888104 11.080544 19 TCCAACG 345 0.0 11.011238 18 GTCCTAA 870 0.0 10.963921 1 CCGGTTT 175 2.0008883E-11 10.853935 12 TGCCGGT 175 2.0008883E-11 10.853935 10 CTAGGAC 330 0.0 10.651638 3 GTAGGAC 1920 0.0 10.291785 3 TGTAGGA 2020 0.0 10.252594 2 CTGTAGG 1980 0.0 10.116708 1 GGCGAGG 695 0.0 10.112563 19 TACACCG 85 6.5947586E-4 10.05894 5 AAGTGCG 85 6.613402E-4 10.055851 7 GGACGTG 1580 0.0 10.038173 6 AGGACGT 1625 0.0 9.997038 5 ACTGTTC 800 0.0 9.972054 8 >>END_MODULE