FastQCFastQC Report
Thu 26 May 2016
SRR1511768_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511768_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2191554
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT77510.3536759760425707No Hit
CTGTAGGACGTGGAATATGGCAAGA69560.3174003469684069No Hit
GTCCTAAAGTGTGTATTTCTCATTT65440.2986009014607899No Hit
CTTTAGGACGTGAAATATGGCGAGG56110.25602837073601653No Hit
GTATCAACGCAGAGTACTTTTTTTT49630.2264603108114151No Hit
TTGTAGAACAGTGTATATCAATGAG42200.19255742728675634No Hit
GATATACACTGTTCTACAAATCCCG37510.17115708761910498No Hit
GTCCTACAGTGTGCATTTCTCATTT35710.16294373764004902No Hit
GTGTATATCAATGAGTTACAATGAA33110.15108000989252374No Hit
CTGAAGGACCTGGAATATGGCGAGA31110.14195406547135048No Hit
TATCAACGCAGAGTACTTTTTTTTT29990.13684353659549342No Hit
GGTATCAACGCAGAGTACTTTTTTT29400.13415138299124732No Hit
CTGTAGGACCTGGAATATGGCGAGA25300.11544319692784208No Hit
GATATACACTGTTCTACAATGCCGG25160.11480438081835993No Hit
ATTTAGAAATGTCCACTGTAGGACG24050.10973948166460876No Hit
GTCCTTCAGTGTGCATTTCTCATTT23950.10928318444355009No Hit
TTTCTAAATTTTCCACCTTTTTCAG23710.10818807111300931No Hit
GTGTATATCAATGAGTTACAATGAG22580.1030319125150464No Hit
GTTCTACAGTGTGGTTTTTATCATT22420.10230183696135255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGA1054.7293724E-1115.0653562
CCGTAGG807.91224E-814.8299611
CGGTAGA551.3972615E-414.38056851
CGTTAGG851.66061E-713.9576091
CGTAGAA851.66061E-713.9576092
GCGTAAC450.00884181513.1821861
CGGTAGG1453.6379788E-1212.9549071
TAGGACC10250.012.8239254
TCCAACG6850.012.70069118
CCAACGT2850.012.60865619
TGTAGAA17600.012.1336052
GGTATCA10050.012.0016921
AAATGTC9400.011.9715167
CCAACGA4300.011.87559519
AATGTCC10000.011.6314858
GGCGAGG16750.011.57361719
TAGAAAT11100.011.4159464
CGTAACT700.001068959711.2990172
TTGTAGA11300.011.2865181
GAAATGT10700.011.224066
Produced by FastQC (version 0.11.5)