##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511768_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2191554 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.939646935462232 31.0 30.0 34.0 25.0 34.0 2 27.23852663452509 31.0 27.0 34.0 10.0 34.0 3 28.943526830732896 31.0 28.0 34.0 16.0 34.0 4 33.48404556766568 35.0 33.0 37.0 28.0 37.0 5 33.949690037297735 37.0 35.0 37.0 30.0 37.0 6 34.21335134794762 37.0 35.0 37.0 30.0 37.0 7 34.2808833366643 37.0 35.0 37.0 30.0 37.0 8 34.30388254179454 37.0 35.0 37.0 30.0 37.0 9 35.964352692199235 39.0 37.0 39.0 31.0 39.0 10 35.833108835100575 39.0 37.0 39.0 31.0 39.0 11 35.85420573711622 39.0 37.0 39.0 31.0 39.0 12 35.77133577361087 39.0 37.0 39.0 30.0 39.0 13 35.77890300672491 39.0 37.0 39.0 30.0 39.0 14 36.97704140532243 40.0 37.0 41.0 31.0 41.0 15 36.94362767241875 40.0 37.0 41.0 31.0 41.0 16 36.893963826581505 40.0 37.0 41.0 31.0 41.0 17 36.88283382476544 40.0 37.0 41.0 31.0 41.0 18 36.83652832647519 40.0 37.0 41.0 31.0 41.0 19 36.874555224283775 40.0 37.0 41.0 31.0 41.0 20 36.811243072267445 40.0 37.0 41.0 31.0 41.0 21 36.732858054147876 40.0 37.0 41.0 30.0 41.0 22 36.64197551144074 40.0 37.0 41.0 30.0 41.0 23 36.55373629853519 40.0 37.0 41.0 30.0 41.0 24 36.47461299151196 40.0 37.0 41.0 30.0 41.0 25 36.38543380633103 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0206047161618415 1101 2 3.6309530509630257 1101 3 2.213468947804788 1101 4 1.1917880106787067 1101 5 0.6786848583241749 1101 6 0.4546972854229452 1101 7 0.29344400466258236 1101 8 0.07818072693926581 1101 9 0.21223786904143793 1101 10 0.4619065472954773 1101 11 0.2832366769152159 1101 12 0.33668062257556386 1101 13 0.369953207383233 1101 14 0.531401008168551 1101 15 0.4136625132221923 1101 16 0.4460058549211894 1101 17 0.41345366038125064 1101 18 0.39255764864057596 1101 19 0.513742316469191 1101 20 0.5021646044533483 1101 21 0.6295355250087411 1101 22 0.45789099243896203 1101 23 0.524170187062893 1101 24 0.5316661054576315 1101 25 0.4306554851888649 1102 1 1.3111115408689713 1102 2 4.048286322881669 1102 3 2.5718031767689773 1102 4 1.462509070286778 1102 5 1.1410555676126322 1102 6 0.8665953575135532 1102 7 0.8325395710339407 1102 8 0.8274578248905726 1102 9 0.833902923572559 1102 10 0.8401263402256234 1102 11 0.84794728579066 1102 12 0.7906042534118782 1102 13 0.8488137602177801 1102 14 0.991350955198385 1102 15 1.0264147612556371 1102 16 0.8752510172350938 1102 17 1.0063971128079032 1102 18 0.9579387011859595 1102 19 0.9182450366280506 1102 20 1.0567648204283344 1102 21 1.1047982007443125 1102 22 0.8248156007923839 1102 23 0.884876558324919 1102 24 0.8368177741458638 1102 25 0.7754273040360076 1103 1 1.4552609067264228 1103 2 4.098819427098864 1103 3 2.6620975841153225 1103 4 1.636325573978361 1103 5 1.1830804530789223 1103 6 0.9446208248383101 1103 7 0.8786754619665089 1103 8 0.614603743888587 1103 9 0.826626994729267 1103 10 0.69256249036399 1103 11 0.8541770834644424 1103 12 0.9442749192688638 1103 13 0.966037782698919 1103 14 1.1116749253617257 1103 15 0.8103633814439277 1103 16 0.7316410170934518 1103 17 0.9413101274395714 1103 18 1.0153554069542068 1103 19 1.048384608467451 1103 20 0.9955323101879188 1103 21 1.0844194396576938 1103 22 1.1152149135338902 1103 23 1.091507586534938 1103 24 1.202562138178827 1103 25 0.9982078276052135 1104 1 1.2930605573948775 1104 2 3.8494476746189363 1104 3 2.351265669712536 1104 4 1.4480470754448973 1104 5 0.9600197374985129 1104 6 0.731909770362364 1104 7 0.6487551051649803 1104 8 0.6609464183621228 1104 9 0.6436484489591408 1104 10 0.49087515308328733 1104 11 0.616704134688753 1104 12 0.6926309737997371 1104 13 0.6617497928932465 1104 14 0.8104953272028581 1104 15 0.8300584303832963 1104 16 0.5906551990754139 1104 17 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-1.1665521342391827 2315 14 -1.2806938971822177 2315 15 -1.414015272956874 2315 16 -1.5897127345293853 2315 17 -1.366733665065766 2315 18 -1.5140384064059305 2315 19 -1.671924251751328 2315 20 -1.5625345281993575 2315 21 -1.689639128663032 2315 22 -1.578903560262198 2315 23 -1.5865018651388851 2315 24 -1.472992112814218 2315 25 -1.5085546336041773 2316 1 -0.29448683365456674 2316 2 2.158607338484483 2316 3 0.6815920328159173 2316 4 -0.2612435245160185 2316 5 -0.4603965220285531 2316 6 -0.6232104172475701 2316 7 -1.1168868725563428 2316 8 -1.09294062482725 2316 9 -1.1874068568841238 2316 10 -1.115613023582732 2316 11 -1.1138020212620034 2316 12 -1.253421400638409 2316 13 -1.3167180593639287 2316 14 -1.3728425660939934 2316 15 -1.472593514115431 2316 16 -1.4890672945732106 2316 17 -1.4687649748734373 2316 18 -1.6317730807186166 2316 19 -1.4965914358000418 2316 20 -1.283612110924885 2316 21 -1.5988145246885566 2316 22 -1.4383849959622665 2316 23 -1.5318731686958316 2316 24 -1.511807530330998 2316 25 -1.752231393902946 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 54828.0 3 1.0 4 505.0 5 2501.0 6 2936.0 7 2778.0 8 2496.0 9 2361.0 10 2271.0 11 2197.0 12 2175.0 13 2183.0 14 2243.0 15 2262.0 16 2354.0 17 2574.0 18 2934.0 19 3618.0 20 4755.0 21 6082.0 22 7438.0 23 9032.0 24 10625.0 25 12347.0 26 14284.0 27 16527.0 28 19397.0 29 24430.0 30 30681.0 31 41277.0 32 54673.0 33 77348.0 34 108702.0 35 163806.0 36 271050.0 37 354163.0 38 871720.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.87841139209894 19.426762927128422 13.6077869858557 25.08703869491694 2 14.999072140702587 21.154476408282896 42.56684522591346 21.27960622510105 3 18.83608617446798 24.9451758888898 30.249813602585196 25.96892433405702 4 11.538068420855703 17.492199599006003 39.95507297561456 31.014659004523732 5 12.22351810633003 38.34101281556375 37.10969476453695 12.325774313569275 6 30.08682423522304 38.553829839465514 19.033389092853746 12.325956832457699 7 26.30275138098354 31.67533175089457 23.327967278013684 18.693949590108204 8 22.77749031052851 40.21032564107478 21.3991532948766 15.613030753520105 9 25.589513194746743 15.722907124351032 21.104978476460083 37.58260120444214 10 14.105607254030703 30.185612583582245 35.85236777190979 19.85641239047726 11 33.70005940989818 22.205293595320946 26.343772501156714 17.75087449362416 12 22.89462180717427 24.31534883466253 34.09151679584441 18.698512562318793 13 29.093373925534117 22.62978690007182 26.423305106787236 21.853534067606823 14 19.365892877839194 23.661657435773883 28.587705345156905 28.38474434123001 15 21.651576917566256 33.63312060756888 23.781846123800737 20.93345635106413 16 20.164778052468705 28.596876919300186 30.896021727048478 20.34232330118263 17 20.061016064399965 29.383031401462155 29.89887540986898 20.657077124268895 18 19.697712216993057 28.21422606972039 32.9573900529031 19.130671660383452 19 22.771239038600008 26.832421195188438 28.18420171257473 22.212138053636828 20 22.194068683682904 28.87193288415435 29.674605325718645 19.259393106444104 21 21.574573635487546 27.63270047893 27.945629398709226 22.84709648687323 22 21.50917344119032 29.90097392523262 28.718656800867333 19.871195832709724 23 21.144098016785435 28.980494564788373 29.007370606006816 20.868036812419376 24 22.34771415521322 27.47307502944259 30.202235583624947 19.976975231719244 25 21.766198779496193 28.354719984084355 29.753636004406005 20.125445232013448 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 182.0 1 182.0 2 337.0 3 492.0 4 492.0 5 492.0 6 1336.0 7 2180.0 8 2180.0 9 2180.0 10 3255.0 11 4330.0 12 4330.0 13 4330.0 14 7290.0 15 10250.0 16 10250.0 17 10250.0 18 19031.5 19 27813.0 20 27813.0 21 27813.0 22 47332.5 23 66852.0 24 66852.0 25 66852.0 26 100323.0 27 133794.0 28 133794.0 29 133794.0 30 178914.5 31 224035.0 32 224035.0 33 224035.0 34 240116.0 35 256197.0 36 256197.0 37 256197.0 38 263564.5 39 270932.0 40 270932.0 41 270932.0 42 277042.0 43 283152.0 44 283152.0 45 283152.0 46 280163.0 47 277174.0 48 277174.0 49 277174.0 50 256308.5 51 235443.0 52 235443.0 53 235443.0 54 200113.5 55 164784.0 56 164784.0 57 164784.0 58 137723.5 59 110663.0 60 110663.0 61 110663.0 62 88771.5 63 66880.0 64 66880.0 65 66880.0 66 50569.5 67 34259.0 68 34259.0 69 34259.0 70 24312.5 71 14366.0 72 14366.0 73 14366.0 74 9818.0 75 5270.0 76 5270.0 77 5270.0 78 3512.5 79 1755.0 80 1755.0 81 1755.0 82 1140.0 83 525.0 84 525.0 85 525.0 86 340.5 87 156.0 88 156.0 89 156.0 90 101.0 91 46.0 92 46.0 93 46.0 94 32.0 95 18.0 96 18.0 97 18.0 98 12.0 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.349927038074353 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 9.125944421173286E-5 22 4.562972210586643E-4 23 5.019269431645307E-4 24 4.562972210586643E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2191554.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.32064149649673 #Duplication Level Percentage of deduplicated Percentage of total 1 86.819009353158 61.91987441157577 2 9.511288829641988 13.5670244157706 3 1.9966327639706787 4.272033886779365 4 0.6240897414659762 1.7804192285094487 5 0.2918059932869562 1.0405895316874068 6 0.16453635979122072 0.7040903237884951 7 0.10284197635247344 0.5134329008358166 8 0.07333834728387155 0.4184430379662861 9 0.057211147837176804 0.3672302188048524 >10 0.3039907468039701 4.030268653558774 >50 0.024633356303737433 1.227947424996327 >100 0.02466868717235675 3.578164573672816 >500 0.0026528323282026646 1.270466365154359 >1k 0.003041051693305274 4.070420842134288 >5k 2.5881291006853396E-4 1.239594184765336 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 7751 0.3536759760425707 No Hit CTGTAGGACGTGGAATATGGCAAGA 6956 0.3174003469684069 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6544 0.2986009014607899 No Hit CTTTAGGACGTGAAATATGGCGAGG 5611 0.25602837073601653 No Hit GTATCAACGCAGAGTACTTTTTTTT 4963 0.2264603108114151 No Hit TTGTAGAACAGTGTATATCAATGAG 4220 0.19255742728675634 No Hit GATATACACTGTTCTACAAATCCCG 3751 0.17115708761910498 No Hit GTCCTACAGTGTGCATTTCTCATTT 3571 0.16294373764004902 No Hit GTGTATATCAATGAGTTACAATGAA 3311 0.15108000989252374 No Hit CTGAAGGACCTGGAATATGGCGAGA 3111 0.14195406547135048 No Hit TATCAACGCAGAGTACTTTTTTTTT 2999 0.13684353659549342 No Hit GGTATCAACGCAGAGTACTTTTTTT 2940 0.13415138299124732 No Hit CTGTAGGACCTGGAATATGGCGAGA 2530 0.11544319692784208 No Hit GATATACACTGTTCTACAATGCCGG 2516 0.11480438081835993 No Hit ATTTAGAAATGTCCACTGTAGGACG 2405 0.10973948166460876 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2395 0.10928318444355009 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2371 0.10818807111300931 No Hit GTGTATATCAATGAGTTACAATGAG 2258 0.1030319125150464 No Hit GTTCTACAGTGTGGTTTTTATCATT 2242 0.10230183696135255 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 4.562972210586643E-5 0.0 12 4.562972210586643E-5 0.0 0.0 4.562972210586643E-5 0.0 13 4.562972210586643E-5 0.0 0.0 4.562972210586643E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTAGGA 105 4.7293724E-11 15.065356 2 CCGTAGG 80 7.91224E-8 14.829961 1 CGGTAGA 55 1.3972615E-4 14.3805685 1 CGTTAGG 85 1.66061E-7 13.957609 1 CGTAGAA 85 1.66061E-7 13.957609 2 GCGTAAC 45 0.008841815 13.182186 1 CGGTAGG 145 3.6379788E-12 12.954907 1 TAGGACC 1025 0.0 12.823925 4 TCCAACG 685 0.0 12.700691 18 CCAACGT 285 0.0 12.608656 19 TGTAGAA 1760 0.0 12.133605 2 GGTATCA 1005 0.0 12.001692 1 AAATGTC 940 0.0 11.971516 7 CCAACGA 430 0.0 11.875595 19 AATGTCC 1000 0.0 11.631485 8 GGCGAGG 1675 0.0 11.573617 19 TAGAAAT 1110 0.0 11.415946 4 CGTAACT 70 0.0010689597 11.299017 2 TTGTAGA 1130 0.0 11.286518 1 GAAATGT 1070 0.0 11.22406 6 >>END_MODULE