FastQCFastQC Report
Thu 26 May 2016
SRR1511768_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511768_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2191554
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT76210.3477441121688081No Hit
CTGTAGGACGTGGAATATGGCAAGA73220.334100825259154No Hit
GTCCTAAAGTGTGTATTTCTCATTT64940.2963194153554966No Hit
GTATCAACGCAGAGTACTTTTTTTT63830.2912545162017455No Hit
CTTTAGGACGTGAAATATGGCGAGG62530.28532265232798276No Hit
TTGTAGAACAGTGTATATCAATGAG48730.2223536358218871No Hit
GATATACACTGTTCTACAAATCCCG42560.1942000972825675No Hit
TATCAACGCAGAGTACTTTTTTTTT42090.1920555003435918No Hit
GGTATCAACGCAGAGTACTTTTTTT39950.1822907398129364No Hit
GTCCTACAGTGTGCATTTCTCATTT34890.159202100427368No Hit
CTGAAGGACCTGGAATATGGCGAGA34830.1589283220947328No Hit
GTGTATATCAATGAGTTACAATGAA34370.15682935487786293No Hit
TTTCTAAATTTTCCACCTTTTTCAG27870.12717003550904973No Hit
CTGTAGGACCTGGAATATGGCGAGA27020.1232915091300511No Hit
ATTTAGAAATGTCCACTGTAGGACG26110.11913920441841724No Hit
GAATATGGCAAGAAAACTGAAAATC26030.11877416664157033No Hit
GATATACACTGTTCTACAATGCCGG25670.11713149664575914No Hit
GTGTATATCAATGAGTTACAATGAG24300.11088022471725542No Hit
GTCCTTCAGTGTGCATTTCTCATTT22710.10362509890242268No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTTCG400.005289828414.2460139
TTACGAC500.001499957913.299923
TCCAACG6500.012.8579418
CCAACGA5150.012.35576519
GGTATCA11400.011.8801651
AATGTCC9400.011.4170188
CCACCTT8500.011.396811513
AAATGTC9350.011.274927
TAGGACC11550.011.2683314
CACCTTT9000.010.76365514
TAGAAAT10250.010.6584744
GGCGAGG16950.010.645987519
TGTAGAA18000.010.5027142
ACTGTTC17550.010.49848658
GAAATGT10700.010.3849446
GTGCTAG1855.638867E-1110.3109171
GTATCAA22950.010.1817491
TCTAGGA4300.010.162732
ATTTAGA10700.010.1615531
TGTAGGA40500.010.1567292
Produced by FastQC (version 0.11.5)