##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511768_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2191554 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.282332992935608 31.0 30.0 34.0 26.0 34.0 2 30.490895501548216 31.0 31.0 34.0 27.0 34.0 3 30.58693511544776 31.0 31.0 34.0 27.0 34.0 4 34.15445250265337 37.0 35.0 37.0 30.0 37.0 5 33.726825804885486 37.0 35.0 37.0 28.0 37.0 6 33.68524526431929 37.0 35.0 37.0 29.0 37.0 7 33.47816480908068 37.0 35.0 37.0 28.0 37.0 8 33.35953255087486 37.0 35.0 37.0 27.0 37.0 9 34.76249090827787 38.0 35.0 39.0 28.0 39.0 10 34.50921218459595 38.0 35.0 39.0 27.0 39.0 11 34.49705825181584 38.0 35.0 39.0 27.0 39.0 12 34.2939142727033 38.0 34.0 39.0 27.0 39.0 13 34.28522226693935 38.0 35.0 39.0 27.0 39.0 14 35.14669864397592 39.0 34.0 40.0 26.0 41.0 15 35.07811625905636 39.0 34.0 40.0 26.0 41.0 16 34.893756211345924 38.0 34.0 40.0 25.0 41.0 17 34.84934890949527 38.0 34.0 40.0 25.0 41.0 18 34.760815841179365 38.0 34.0 40.0 25.0 41.0 19 34.786685155830064 39.0 34.0 40.0 25.0 41.0 20 34.893965195473164 39.0 34.0 40.0 25.0 41.0 21 34.82121088506147 39.0 34.0 40.0 25.0 41.0 22 34.687984416537304 39.0 34.0 40.0 24.0 41.0 23 34.554703192346615 39.0 34.0 40.0 24.0 41.0 24 34.36448291942612 39.0 34.0 40.0 21.0 41.0 25 33.811694806516286 38.0 33.0 40.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.39405557185153484 1101 2 0.43019534663873316 1101 3 0.43835927784502005 1101 4 0.4826266259711076 1101 5 0.6967970611670182 1101 6 0.5119838589042871 1101 7 0.578976304936873 1101 8 0.3793347434213601 1101 9 0.5577328172968237 1101 10 0.6230343725024383 1101 11 0.6472727432254786 1101 12 0.6318819951918542 1101 13 0.5641344411403253 1101 14 0.3558654289497909 1101 15 0.6174263404195202 1101 16 0.46777022699225057 1101 17 0.3680745435782171 1101 18 0.6178591061549952 1101 19 0.5180029967480877 1101 20 0.3673435888542542 1101 21 0.654123764840854 1101 22 0.47295621034353985 1101 23 0.5781970843363524 1101 24 0.2977878800250906 1101 25 0.5776196889025442 1102 1 0.611901144620262 1102 2 0.6005699250524934 1102 3 0.5771539122485052 1102 4 0.696068253869754 1102 5 0.8640394905273325 1102 6 0.7743985820519939 1102 7 0.8622899343913559 1102 8 0.8672027837298657 1102 9 0.8983002890076861 1102 10 0.900713204395089 1102 11 0.8983769281246339 1102 12 1.064412843277509 1102 13 1.1183456974289356 1102 14 1.230750916147386 1102 15 1.1760671310312603 1102 16 1.0470745009426992 1102 17 1.3955601751003286 1102 18 1.2418937440386486 1102 19 1.3940809524578626 1102 20 1.2761018350554991 1102 21 1.0061757830810123 1102 22 1.1818608282162373 1102 23 1.4715018598400817 1102 24 1.426331379536336 1102 25 1.5471784755530393 1103 1 0.3735311757119213 1103 2 0.5241099447970115 1103 3 0.6820586329238374 1103 4 0.6242447053739397 1103 5 0.8173511937741225 1103 6 0.8052948621822225 1103 7 0.679523197360588 1103 8 0.826569367382028 1103 9 0.7949363241683045 1103 10 0.9336680224552723 1103 11 0.8879603574788533 1103 12 0.9612422317168239 1103 13 1.0285931289217345 1103 14 1.349463084386322 1103 15 1.1450512416635092 1103 16 1.2815409819152777 1103 17 1.3581038229167746 1103 18 1.106963507475406 1103 19 1.3291974376909508 1103 20 1.2207843613374934 1103 21 1.3497818711912046 1103 22 1.3365969605706027 1103 23 1.0610344327854193 1103 24 1.046033359181628 1103 25 1.2211551701877355 1104 1 0.3487374241327892 1104 2 0.4690750665687169 1104 3 0.46044116195241 1104 4 0.5797754586338186 1104 5 0.45653608683252855 1104 6 0.514281251434241 1104 7 0.5845590642630683 1104 8 0.6056613250667695 1104 9 0.5349789470110693 1104 10 0.7787801271229142 1104 11 0.6706576803688549 1104 12 0.8227210685965716 1104 13 0.644179907052262 1104 14 1.0021570018430097 1104 15 0.9466377341770453 1104 16 1.1368975088127087 1104 17 1.0624654140231016 1104 18 1.2274833303928716 1104 19 0.9027855560242699 1104 20 0.928420221648814 1104 21 0.880777246393059 1104 22 0.8515481310509898 1104 23 0.9878094874371328 1104 24 0.9848937474010242 1104 25 1.1448710471966663 1105 1 0.5779993795108282 1105 2 0.5333586028267412 1105 3 0.5144350205278592 1105 4 0.5529103767123686 1105 5 0.540414632646268 1105 6 0.5921064140960937 1105 7 0.6540542219860157 1105 8 0.5646473458101795 1105 9 0.5728328841795758 1105 10 0.7389446142280676 1105 11 0.7891210358199103 1105 12 0.7098193528629224 1105 13 0.8078827995343829 1105 14 0.8437872709061196 1105 15 0.7499665580727708 1105 16 0.845082352807637 1105 17 0.9613732711812943 1105 18 0.9941422665950697 1105 19 0.7478928162188154 1105 20 0.8422964053761888 1105 21 0.894360101940741 1105 22 0.8076244908611585 1105 23 1.0878923751950822 1105 24 1.1150747691107483 1105 25 1.3042642057356062 1106 1 0.3283283006075983 1106 2 0.4490574217933343 1106 3 0.5132209198660931 1106 4 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1.3776837411999239 1107 17 1.2373600311087003 1107 18 1.2349692027796664 1107 19 1.1552419981090694 1107 20 1.1581603162958132 1107 21 1.1143369776212282 1107 22 1.221129483669806 1107 23 1.1207633069668859 1107 24 1.2039235622732107 1107 25 1.2102887865649024 1108 1 0.47046352232400324 1108 2 0.47729212083228134 1108 3 0.3606375443813512 1108 4 0.40680388240642884 1108 5 0.5566497819229639 1108 6 0.6345293134497396 1108 7 0.5973549298961416 1108 8 0.5945605498729734 1108 9 0.6379556911971136 1108 10 0.6027697912056027 1108 11 0.6358566467771283 1108 12 0.6258735233769315 1108 13 0.5213478657085915 1108 14 0.8177435651505007 1108 15 0.8972217135052176 1108 16 0.9481429868488789 1108 17 0.8160054657026876 1108 18 0.7125479298760808 1108 19 0.8668475715281474 1108 20 0.8792419270751708 1108 21 0.9383896494938355 1108 22 1.079496451464422 1108 23 0.8964193065585775 1108 24 1.0721495613545144 1108 25 0.9099976621837484 1109 1 -0.17857416306379292 1109 2 -0.02019876173518753 1109 3 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-1.6117352429322125 2315 13 -1.5945096817678461 2315 14 -1.8319426492711912 2315 15 -1.6191926431832755 2315 16 -1.5209693314870094 2315 17 -1.7366527503905473 2315 18 -1.5708155840766551 2315 19 -1.6369641014068037 2315 20 -1.5428302278210566 2315 21 -1.8299892333589298 2315 22 -1.8650388134285478 2315 23 -1.9014658213608158 2315 24 -1.8966561218882774 2315 25 -1.8694499860495277 2316 1 -1.3990640220270727 2316 2 -1.4269655822489504 2316 3 -1.3719469560855018 2316 4 -1.4766228216085082 2316 5 -1.7017461097670434 2316 6 -1.641313823805156 2316 7 -1.7579541270599712 2316 8 -2.651753175617227 2316 9 -2.214178762584396 2316 10 -2.1270826831174645 2316 11 -2.1633739788064403 2316 12 -2.1691218080889882 2316 13 -2.453994804692904 2316 14 -2.2106747355030905 2316 15 -2.3370714704350277 2316 16 -2.1712557059615776 2316 17 -2.20019434755595 2316 18 -2.3325967946804127 2316 19 -2.082625817827598 2316 20 -2.358847708779173 2316 21 -2.313952087200846 2316 22 -2.376757516789546 2316 23 -2.487209078128629 2316 24 -2.478825228561451 2316 25 -2.651544821476385 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 50596.0 3 2.0 4 867.0 5 7461.0 6 9190.0 7 7413.0 8 6008.0 9 5364.0 10 4965.0 11 4831.0 12 4431.0 13 4378.0 14 4312.0 15 4260.0 16 4193.0 17 4469.0 18 4742.0 19 5530.0 20 6553.0 21 8564.0 22 10952.0 23 13431.0 24 16431.0 25 19192.0 26 22164.0 27 25111.0 28 29504.0 29 35422.0 30 44536.0 31 57517.0 32 73329.0 33 94745.0 34 124478.0 35 168728.0 36 251778.0 37 431701.0 38 624406.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.26590449628909 21.099881853818495 14.706611284403376 23.927602365489037 2 14.007548981225195 21.870280175619676 42.87167918289944 21.25049166025569 3 19.362516278403362 25.952543263821013 30.89401401927582 23.790926438499802 4 12.651388010516737 18.85219346637135 39.22276156553751 29.273656957574396 5 12.210156834821346 38.94843986781326 36.84011611984444 12.001287177520952 6 30.208792482412022 39.283220947327784 18.490304140349725 12.017682429910465 7 26.361887500832744 32.08996903567058 23.257971284303284 18.290172179193394 8 23.044378555125725 40.58065646568599 21.05081599632042 15.324148982867863 9 25.680681379514265 15.649717050093221 20.980683113443703 37.68891845694881 10 14.204213083501479 30.823972395843313 35.48167190952174 19.49014261113347 11 34.047894781511204 22.318820343920343 26.11375307202104 17.519531802547416 12 22.990170445263956 24.741119771632366 33.926017793766434 18.342691989337247 13 29.325218543554026 22.805370070735194 26.40487069905647 21.46454068665431 14 19.576428415635664 23.88743330075371 28.511458079518004 28.024680204092622 15 21.71787690378608 33.95531207535839 23.74757820249923 20.57923281835629 16 20.423498576808967 29.080825751955004 30.37949327281007 20.11618239842596 17 20.153598770552765 29.811585751480457 29.557975756016052 20.476839721950725 18 19.875805022372255 28.564023519383962 32.66608990697925 18.894081551264534 19 23.05094923510897 26.9915776658937 28.292846080908795 21.664627018088535 20 22.469945983534974 29.14598499512218 29.41031797528147 18.97375104606138 21 21.810687758549413 27.786858092476844 27.961300520087573 22.44115362888617 22 21.765696852553027 29.915256480104986 28.747044334750594 19.572002332591392 23 21.35302164582757 29.237198809611808 28.850669433653014 20.559110110907604 24 22.550163034997084 27.78215823109994 30.027094928986465 19.64058380491651 25 22.02433753499867 28.63443138800974 29.577250774807528 19.763980302184063 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 157.0 1 157.0 2 372.5 3 588.0 4 588.0 5 588.0 6 1493.5 7 2399.0 8 2399.0 9 2399.0 10 3587.5 11 4776.0 12 4776.0 13 4776.0 14 7841.5 15 10907.0 16 10907.0 17 10907.0 18 20005.5 19 29104.0 20 29104.0 21 29104.0 22 49774.0 23 70444.0 24 70444.0 25 70444.0 26 105335.0 27 140226.0 28 140226.0 29 140226.0 30 185068.5 31 229911.0 32 229911.0 33 229911.0 34 244452.0 35 258993.0 36 258993.0 37 258993.0 38 265737.5 39 272482.0 40 272482.0 41 272482.0 42 277758.0 43 283034.0 44 283034.0 45 283034.0 46 278099.0 47 273164.0 48 273164.0 49 273164.0 50 251505.5 51 229847.0 52 229847.0 53 229847.0 54 195565.5 55 161284.0 56 161284.0 57 161284.0 58 134158.0 59 107032.0 60 107032.0 61 107032.0 62 85215.0 63 63398.0 64 63398.0 65 63398.0 66 47994.0 67 32590.0 68 32590.0 69 32590.0 70 23261.5 71 13933.0 72 13933.0 73 13933.0 74 9434.5 75 4936.0 76 4936.0 77 4936.0 78 3337.0 79 1738.0 80 1738.0 81 1738.0 82 1098.0 83 458.0 84 458.0 85 458.0 86 297.0 87 136.0 88 136.0 89 136.0 90 76.0 91 16.0 92 16.0 93 16.0 94 8.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39538154204733267 2 0.0 3 0.0 4 0.0 5 0.03257962158358863 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0019164483284463902 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2191554.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.29526555079279 #Duplication Level Percentage of deduplicated Percentage of total 1 86.7804876815512 61.002574262019436 2 9.502556144318968 13.359694151524387 3 1.9837878353959715 4.1835267805677665 4 0.6681653009165652 1.8787542903902128 5 0.28273808134227757 0.9937574254638527 6 0.16096433327856158 0.6789018331213678 7 0.1116128060622323 0.5492096288709628 8 0.06946305019235419 0.39063388473916677 9 0.052320233127009626 0.3310078213208275 >10 0.3302977182932316 4.284807364776294 >50 0.026525435669887267 1.3079116862311262 >100 0.02442874473900233 3.6188610542937343 >500 0.0031648458303288347 1.4829459741084707 >1k 0.0031648458303285654 4.390404529738088 >5k 3.229434520743434E-4 1.5470093128343196 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 7621 0.3477441121688081 No Hit CTGTAGGACGTGGAATATGGCAAGA 7322 0.334100825259154 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6494 0.2963194153554966 No Hit GTATCAACGCAGAGTACTTTTTTTT 6383 0.2912545162017455 No Hit CTTTAGGACGTGAAATATGGCGAGG 6253 0.28532265232798276 No Hit TTGTAGAACAGTGTATATCAATGAG 4873 0.2223536358218871 No Hit GATATACACTGTTCTACAAATCCCG 4256 0.1942000972825675 No Hit TATCAACGCAGAGTACTTTTTTTTT 4209 0.1920555003435918 No Hit GGTATCAACGCAGAGTACTTTTTTT 3995 0.1822907398129364 No Hit GTCCTACAGTGTGCATTTCTCATTT 3489 0.159202100427368 No Hit CTGAAGGACCTGGAATATGGCGAGA 3483 0.1589283220947328 No Hit GTGTATATCAATGAGTTACAATGAA 3437 0.15682935487786293 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2787 0.12717003550904973 No Hit CTGTAGGACCTGGAATATGGCGAGA 2702 0.1232915091300511 No Hit ATTTAGAAATGTCCACTGTAGGACG 2611 0.11913920441841724 No Hit GAATATGGCAAGAAAACTGAAAATC 2603 0.11877416664157033 No Hit GATATACACTGTTCTACAATGCCGG 2567 0.11713149664575914 No Hit GTGTATATCAATGAGTTACAATGAG 2430 0.11088022471725542 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2271 0.10362509890242268 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 4.562972210586643E-5 0.0 12 0.0 0.0 0.0 4.562972210586643E-5 0.0 13 0.0 0.0 0.0 4.562972210586643E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGTTCG 40 0.0052898284 14.246013 9 TTACGAC 50 0.0014999579 13.29992 3 TCCAACG 650 0.0 12.85794 18 CCAACGA 515 0.0 12.355765 19 GGTATCA 1140 0.0 11.880165 1 AATGTCC 940 0.0 11.417018 8 CCACCTT 850 0.0 11.3968115 13 AAATGTC 935 0.0 11.27492 7 TAGGACC 1155 0.0 11.268331 4 CACCTTT 900 0.0 10.763655 14 TAGAAAT 1025 0.0 10.658474 4 GGCGAGG 1695 0.0 10.6459875 19 TGTAGAA 1800 0.0 10.502714 2 ACTGTTC 1755 0.0 10.4984865 8 GAAATGT 1070 0.0 10.384944 6 GTGCTAG 185 5.638867E-11 10.310917 1 GTATCAA 2295 0.0 10.181749 1 TCTAGGA 430 0.0 10.16273 2 ATTTAGA 1070 0.0 10.161553 1 TGTAGGA 4050 0.0 10.156729 2 >>END_MODULE