FastQCFastQC Report
Thu 26 May 2016
SRR1511767_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511767_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1970667
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50870.2581359509242302No Hit
TATCAACGCAGAGTACTTTTTTTTT31960.1621785923243247No Hit
GGTATCAACGCAGAGTACTTTTTTT31420.1594384033426246No Hit
GTCCTACAGTGGACATTTCTAAATT27540.13974963806670532No Hit
CTGTAGGACGTGGAATATGGCAAGA25710.1304634420731661No Hit
GTCCTAAAGTGTGTATTTCTCATTT24640.12503380835016772No Hit
CTTTAGGACGTGAAATATGGCGAGG22190.11260146945171355No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG400.004052019714.829331
GGTATCA10450.014.0015361
TAGGACC3300.013.181844
TCCAACG1700.012.79413918
CCGTACT751.4318434E-411.8634652
GGCGAGG6700.011.85595719
CCGGTTT1302.8121576E-911.63881612
TGTCGAG658.339739E-411.63881618
CCAACGA907.854029E-611.5579919
TCCGAAT752.1645818E-411.34784517
CCAACGT1002.0396856E-611.34784519
GCCGGTT1355.0822564E-911.20774711
GATCTCG600.006080596711.03262619
AATACGT600.006080596711.0326265
GTCGAGG700.001549863610.80747119
GCGATTT700.001549863610.80747111
TGGCGAG14300.010.38235318
GTATCAA21550.010.3220391
GTATTAA2700.010.2523771
GTCCTAC12350.010.2464471
Produced by FastQC (version 0.11.5)