##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511767_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1970667 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.498355125447375 31.0 30.0 34.0 25.0 34.0 2 26.82740412256358 31.0 26.0 34.0 10.0 34.0 3 28.496235538525788 31.0 28.0 34.0 16.0 34.0 4 33.01643961156299 35.0 33.0 37.0 27.0 37.0 5 33.47001954160698 35.0 35.0 37.0 28.0 37.0 6 33.72338045950939 37.0 35.0 37.0 29.0 37.0 7 33.771603218605684 37.0 35.0 37.0 29.0 37.0 8 33.793746482789835 37.0 35.0 37.0 29.0 37.0 9 35.39694174611946 39.0 35.0 39.0 30.0 39.0 10 35.27634450670763 39.0 35.0 39.0 29.0 39.0 11 35.29115979513535 39.0 35.0 39.0 30.0 39.0 12 35.200608220465455 39.0 35.0 39.0 29.0 39.0 13 35.19689577183766 39.0 35.0 39.0 29.0 39.0 14 36.35059246437881 40.0 36.0 41.0 29.0 41.0 15 36.29020834062782 40.0 36.0 41.0 29.0 41.0 16 36.22809942014557 40.0 36.0 41.0 28.0 41.0 17 36.20227161666583 40.0 36.0 41.0 28.0 41.0 18 36.14281053064774 40.0 36.0 41.0 28.0 41.0 19 36.17422476755332 40.0 36.0 41.0 28.0 41.0 20 36.10656442717111 40.0 36.0 41.0 27.0 41.0 21 36.02160080825426 40.0 36.0 41.0 27.0 41.0 22 35.91755329540709 40.0 36.0 41.0 27.0 41.0 23 35.81535236546814 40.0 36.0 41.0 27.0 41.0 24 35.70717274912504 40.0 36.0 41.0 27.0 41.0 25 35.601551149940605 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0265903101323133 1101 2 3.6100556025754003 1101 3 2.1249606757187607 1101 4 1.2483313783334395 1101 5 0.7446109280201796 1101 6 0.5535116228472745 1101 7 0.4855155760885239 1101 8 0.22762969190606697 1101 9 0.32856890303882835 1101 10 0.4228393631261582 1101 11 0.2301368066035394 1101 12 0.29682173531394795 1101 13 0.31246285255107153 1101 14 0.4735145594269383 1101 15 0.418431466393578 1101 16 0.2876546240525002 1101 17 0.5362284356694005 1101 18 0.4306040759135925 1101 19 0.5262613412004811 1101 20 0.6170724681533883 1101 21 0.5994960351155711 1101 22 0.6002769190080812 1101 23 0.5904358324171497 1101 24 0.7760366339738027 1101 25 0.5148812111026118 1102 1 1.4168996535666558 1102 2 3.9745652827100812 1102 3 2.66679569255378 1102 4 1.5806966981987642 1102 5 1.0090153892996412 1102 6 0.7731980706587223 1102 7 0.7760879666609171 1102 8 0.665140213791588 1102 9 0.7980238693687909 1102 10 0.8960506425874399 1102 11 0.8753598705766024 1102 12 0.8635199676371812 1102 13 0.8040137784769925 1102 14 0.8557010072383804 1102 15 0.9544584024205136 1102 16 0.9754176711905487 1102 17 0.9943934188343846 1102 18 0.9802778974624147 1102 19 0.9481363412004811 1102 20 0.874942838523765 1102 21 0.9842919105364416 1102 22 0.8249507405569005 1102 23 0.7595435765248908 1102 24 0.8195025430647149 1102 25 0.7398812111026132 1103 1 1.6266349252781858 1103 2 4.047682982049849 1103 3 2.683498333488263 1103 4 1.6497926860098318 1103 5 1.2386021084464218 1103 6 1.1227536826445004 1103 7 1.0103280631565994 1103 8 0.9126357699114465 1103 9 1.0017694254886464 1103 10 0.9061985603121769 1103 11 1.1018326994237313 1103 12 1.0604409935386556 1103 13 1.1372540019406827 1103 14 1.2657314795593564 1103 15 0.9404157411711509 1103 16 1.0089308758629727 1103 17 1.2665874259445928 1103 18 1.2647592331658117 1103 19 1.4528151121502049 1103 20 1.2390140675740398 1103 21 1.318960524045842 1103 22 1.3304516293329272 1103 23 1.1939106410246367 1103 24 1.395083421683303 1103 25 1.255314171996467 1104 1 1.5203316201311061 1104 2 4.089195191702068 1104 3 2.4667598334183545 1104 4 1.7347623822426854 1104 5 1.1571636977376727 1104 6 1.1391566224442542 1104 7 0.8587612033806664 1104 8 0.9589619320624365 1104 9 1.0096842709911087 1104 10 0.8912782862712874 1104 11 1.115188241335332 1104 12 0.9853210854529877 1104 13 0.8519463138415233 1104 14 1.0668445427018298 1104 15 0.9404461361231569 1104 16 0.7921589413458605 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-1.407961397426142 2315 12 -1.5255288398001454 2315 13 -1.3176542852279525 2315 14 -1.4434950607338806 2315 15 -1.5412018886714591 2315 16 -1.6663134497421623 2315 17 -1.5903471130561897 2315 18 -1.691799276202147 2315 19 -1.6798498281205596 2315 20 -1.7299974380898178 2315 21 -1.7627943121260543 2315 22 -1.577396760014615 2315 23 -1.4510785182705064 2315 24 -1.4196229427235991 2315 25 -1.4324679087632717 2316 1 -0.30114308585766025 2316 2 2.1034200901833096 2316 3 0.6382404028142794 2316 4 -0.37894862723603495 2316 5 -0.5118592404565661 2316 6 -0.5410781501964479 2316 7 -1.1246121738418609 2316 8 -1.0494127405640015 2316 9 -1.1368872721212995 2316 10 -1.3510049699286952 2316 11 -1.2828557442179616 2316 12 -1.2911194718706795 2316 13 -1.3951090285405456 2316 14 -1.7537506522132773 2316 15 -1.6713067680815001 2316 16 -1.6905417349004352 2316 17 -1.594689136037644 2316 18 -1.674741753938818 2316 19 -1.586899164229763 2316 20 -1.5643794254695536 2316 21 -1.8035384608465606 2316 22 -1.6560192032419891 2316 23 -1.718626754605964 2316 24 -1.5753496442216885 2316 25 -1.494256215797975 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 61641.0 3 3.0 4 515.0 5 2763.0 6 3293.0 7 2983.0 8 2841.0 9 2746.0 10 2606.0 11 2489.0 12 2513.0 13 2416.0 14 2543.0 15 2688.0 16 2764.0 17 3081.0 18 3450.0 19 4331.0 20 5516.0 21 7144.0 22 8850.0 23 10529.0 24 12301.0 25 14400.0 26 15987.0 27 18337.0 28 21667.0 29 26267.0 30 33294.0 31 42651.0 32 55487.0 33 74647.0 34 102492.0 35 147542.0 36 235427.0 37 300396.0 38 732067.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.66839603037956 18.95378569793882 13.287886791629433 25.089931480052186 2 16.428233044793902 21.23897420227256 39.63435757138818 22.69843518154536 3 18.523626772052303 24.680983646653644 29.886277082835406 26.909112498458644 4 12.386009406967286 17.17692537602751 38.53172555282044 31.90533966418477 5 13.322494363583498 37.58940500855802 35.8096522649438 13.278448362914688 6 31.256828271849074 37.28483807766609 18.472121368044423 12.986212282440412 7 27.566351900143456 31.714084622110178 22.656745152783298 18.06281832496307 8 24.81276643897726 36.9127305627993 20.963105385131023 17.31139761309242 9 26.167282448023943 15.641658382669421 20.588714379446145 37.60234478986049 10 15.072510982322228 29.112021462783922 34.50912812768468 21.306339427209164 11 34.01873578844118 22.65161998450271 24.726501230294108 18.60314299676201 12 22.993077978166784 24.744261714434757 31.937765233801553 20.32489507359691 13 28.689271195996074 22.04035486462198 25.972779774563637 23.297594164818307 14 20.970869253912507 22.09764511203567 27.97773545708128 28.953750176970537 15 22.99378839753241 30.397931258807297 23.913071056652395 22.6952092870079 16 22.238866333074032 28.096832189304433 27.519920920175757 22.14438055744578 17 21.71569321453092 28.301331478123902 27.8934492737738 22.089526033571374 18 21.576095809185418 27.591216577940365 29.522745344596522 21.309942268277695 19 23.241674011895462 27.07453872216869 27.795715866759835 21.888071399176017 20 23.29713746665469 27.399555581942558 28.245665046403072 21.057641904999677 21 23.068835209267135 26.80062496733333 27.50363710081328 22.626902722586255 22 23.059845584336173 27.663086638706417 27.678665215372554 21.598402561584855 23 22.534639498034917 27.38866011554534 27.863273886093708 22.213426500326033 24 22.87700998242217 26.88794233821697 28.310863749477335 21.92418392988353 25 22.77863281822855 27.232556286780056 28.122052076784154 21.866758818207234 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 312.0 1 312.0 2 460.5 3 609.0 4 609.0 5 609.0 6 1452.5 7 2296.0 8 2296.0 9 2296.0 10 3388.5 11 4481.0 12 4481.0 13 4481.0 14 7014.5 15 9548.0 16 9548.0 17 9548.0 18 16827.5 19 24107.0 20 24107.0 21 24107.0 22 38026.5 23 51946.0 24 51946.0 25 51946.0 26 73805.5 27 95665.0 28 95665.0 29 95665.0 30 123185.5 31 150706.0 32 150706.0 33 150706.0 34 169644.0 35 188582.0 36 188582.0 37 188582.0 38 202561.5 39 216541.0 40 216541.0 41 216541.0 42 229315.0 43 242089.0 44 242089.0 45 242089.0 46 248225.5 47 254362.0 48 254362.0 49 254362.0 50 246578.0 51 238794.0 52 238794.0 53 238794.0 54 214459.0 55 190124.0 56 190124.0 57 190124.0 58 162683.0 59 135242.0 60 135242.0 61 135242.0 62 110788.0 63 86334.0 64 86334.0 65 86334.0 66 66129.5 67 45925.0 68 45925.0 69 45925.0 70 33383.5 71 20842.0 72 20842.0 73 20842.0 74 14495.0 75 8148.0 76 8148.0 77 8148.0 78 5500.0 79 2852.0 80 2852.0 81 2852.0 82 1840.0 83 828.0 84 828.0 85 828.0 86 530.0 87 232.0 88 232.0 89 232.0 90 151.5 91 71.0 92 71.0 93 71.0 94 49.0 95 27.0 96 27.0 97 27.0 98 15.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.346548655861189 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 2.0297696160741517E-4 22 6.089308848222455E-4 23 6.089308848222455E-4 24 1.5223272120556137E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1970667.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.64980985430648 #Duplication Level Percentage of deduplicated Percentage of total 1 83.59084726642274 61.56450006732409 2 11.554199344527879 17.01929169486462 3 2.7494646667627434 6.074926497246305 4 0.923340006350434 2.7201526359433443 5 0.40351586289277264 1.4859433287624555 6 0.21602153793556872 0.9545967116033693 7 0.12580884883362395 0.6486058456209914 8 0.08478183005156624 0.4995332529918385 9 0.05902044950846878 0.3912160395432979 >10 0.2587336060952189 3.3468744312120275 >50 0.017280454738964272 0.8727483320624332 >100 0.014202976701777646 2.039864553657133 >500 0.0018786256953462548 0.9242988852697049 >1k 8.349447534871922E-4 1.1967664338076203 >5k 6.957872945726602E-5 0.2606812900907496 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5087 0.2581359509242302 No Hit TATCAACGCAGAGTACTTTTTTTTT 3196 0.1621785923243247 No Hit GGTATCAACGCAGAGTACTTTTTTT 3142 0.1594384033426246 No Hit GTCCTACAGTGGACATTTCTAAATT 2754 0.13974963806670532 No Hit CTGTAGGACGTGGAATATGGCAAGA 2571 0.1304634420731661 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2464 0.12503380835016772 No Hit CTTTAGGACGTGAAATATGGCGAGG 2219 0.11260146945171355 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.0744240401853786E-5 0.0 5 0.0 0.0 0.0 5.0744240401853786E-5 5.0744240401853786E-5 6 0.0 0.0 0.0 5.0744240401853786E-5 1.5223272120556137E-4 7 0.0 0.0 0.0 5.0744240401853786E-5 1.5223272120556137E-4 8 0.0 0.0 0.0 5.0744240401853786E-5 1.5223272120556137E-4 9 0.0 0.0 0.0 1.0148848080370757E-4 2.0297696160741514E-4 10 0.0 0.0 0.0 2.0297696160741514E-4 2.0297696160741514E-4 11 0.0 0.0 0.0 2.5372120200926895E-4 2.0297696160741514E-4 12 0.0 0.0 0.0 2.5372120200926895E-4 3.0446544241112273E-4 13 0.0 0.0 0.0 2.5372120200926895E-4 3.0446544241112273E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTAGG 40 0.0040520197 14.82933 1 GGTATCA 1045 0.0 14.001536 1 TAGGACC 330 0.0 13.18184 4 TCCAACG 170 0.0 12.794139 18 CCGTACT 75 1.4318434E-4 11.863465 2 GGCGAGG 670 0.0 11.855957 19 CCGGTTT 130 2.8121576E-9 11.638816 12 TGTCGAG 65 8.339739E-4 11.638816 18 CCAACGA 90 7.854029E-6 11.55799 19 TCCGAAT 75 2.1645818E-4 11.347845 17 CCAACGT 100 2.0396856E-6 11.347845 19 GCCGGTT 135 5.0822564E-9 11.207747 11 GATCTCG 60 0.0060805967 11.032626 19 AATACGT 60 0.0060805967 11.032626 5 GTCGAGG 70 0.0015498636 10.807471 19 GCGATTT 70 0.0015498636 10.807471 11 TGGCGAG 1430 0.0 10.382353 18 GTATCAA 2155 0.0 10.322039 1 GTATTAA 270 0.0 10.252377 1 GTCCTAC 1235 0.0 10.246447 1 >>END_MODULE