FastQCFastQC Report
Thu 26 May 2016
SRR1511767_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511767_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1970667
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64010.3248138828122661No Hit
TATCAACGCAGAGTACTTTTTTTTT41940.2128213442453748No Hit
GGTATCAACGCAGAGTACTTTTTTT38450.1951116043451278No Hit
GTCCTACAGTGGACATTTCTAAATT28200.14309875793322768No Hit
CTGTAGGACGTGGAATATGGCAAGA26850.13624828547897744No Hit
GTCCTAAAGTGTGTATTTCTCATTT26800.13599456427696816No Hit
CTTTAGGACGTGAAATATGGCGAGG25070.12721581068744744No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19960.10128550384210017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTTC1600.012.46555313
TCCAACG2450.012.40498218
GGTATCA12150.012.2413031
CGTGCGC701.0938017E-412.21115410
CCCGTTT1657.2759576E-1211.51219812
GGCGAGG6950.011.20604319
TAGGACC3400.011.1764414
AGGGCCG600.005876723711.0833035
GCACCGT600.00588783411.0804916
TGGACCG700.001491742710.8571145
CGTTTCC1752.0008883E-1110.85435914
CTAGGAC1951.8189894E-1210.717923
GCCGGTT1605.475158E-1010.6847611
TCCCGTT1856.002665E-1110.26763711
CGGTTTC1606.199116E-910.09116213
CCGGTTT1606.199116E-910.09116212
TGGCGAG14850.09.9772418
TAGACTG2002.5465852E-119.9749745
ATCCCGT2107.2759576E-129.94982910
GATATAC5950.09.9346841
Produced by FastQC (version 0.11.5)