##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511767_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1970667 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.20333623082946 31.0 31.0 34.0 28.0 34.0 2 31.384550002613327 33.0 31.0 34.0 28.0 34.0 3 31.490655194408795 34.0 31.0 34.0 28.0 34.0 4 35.04395364615128 37.0 35.0 37.0 32.0 37.0 5 34.70356128153564 37.0 35.0 37.0 32.0 37.0 6 34.66288672819913 37.0 35.0 37.0 32.0 37.0 7 34.503183439921614 37.0 35.0 37.0 31.0 37.0 8 34.42067888689464 37.0 35.0 37.0 30.0 37.0 9 35.96625609501758 39.0 37.0 39.0 31.0 39.0 10 35.786081057834735 39.0 35.0 39.0 30.0 39.0 11 35.78372956973451 39.0 35.0 39.0 30.0 39.0 12 35.62970151730353 39.0 35.0 39.0 30.0 39.0 13 35.61132246087239 39.0 35.0 39.0 30.0 39.0 14 36.68735966046014 40.0 36.0 41.0 30.0 41.0 15 36.61286610066541 40.0 36.0 41.0 30.0 41.0 16 36.46806842556353 40.0 36.0 41.0 30.0 41.0 17 36.44389234710887 40.0 36.0 41.0 30.0 41.0 18 36.37344817769821 40.0 36.0 41.0 30.0 41.0 19 36.39506826876382 40.0 36.0 41.0 30.0 41.0 20 36.49633905677621 40.0 37.0 41.0 30.0 41.0 21 36.43826836294514 40.0 37.0 41.0 30.0 41.0 22 36.34437578748718 40.0 36.0 41.0 30.0 41.0 23 36.240268903878736 40.0 36.0 41.0 30.0 41.0 24 36.099982899190984 40.0 36.0 41.0 29.0 41.0 25 35.59977865362337 39.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18114211292790827 1101 2 0.2114153018100211 1101 3 0.2700572108476429 1101 4 0.3690844803818436 1101 5 0.35462770683380995 1101 6 0.37188263816419465 1101 7 0.5467618885445589 1101 8 0.3532494360945222 1101 9 0.3138902260150971 1101 10 0.4372466750216901 1101 11 0.42355563866212975 1101 12 0.38535586340759664 1101 13 0.4705149326201763 1101 14 0.48967013555525085 1101 15 0.3072633997445777 1101 16 0.22851244510864177 1101 17 0.2998378371052297 1101 18 0.32701877845202887 1101 19 0.21595598145634654 1101 20 -0.02348014209740512 1101 21 0.12893511422105064 1101 22 0.10900756839205883 1101 23 0.18894285950268852 1101 24 -0.04002765531298991 1101 25 0.26404853317405497 1102 1 0.3627498570356522 1102 2 0.3845213624160806 1102 3 0.344615291655721 1102 4 0.4538908776882451 1102 5 0.43167947451057387 1102 6 0.5693152980968534 1102 7 0.5495817538644303 1102 8 0.6501875674076558 1102 9 0.6306095021093725 1102 10 0.7461692339442507 1102 11 0.6188839214904149 1102 12 0.5643226142493489 1102 13 0.6419585353137762 1102 14 0.8530960614811747 1102 15 0.7279389047950815 1102 16 0.7016185057147055 1102 17 0.7624009684183619 1102 18 0.84169680875506 1102 19 1.0612106110859756 1102 20 0.8654087467914806 1102 21 0.8452966462075864 1102 22 0.9195820633415508 1102 23 1.0477601995700283 1102 24 0.7940001224647872 1102 25 1.0018010584265795 1103 1 0.5052174801946165 1103 2 0.5620321546964249 1103 3 0.5231608731691786 1103 4 0.5335759970381702 1103 5 0.6604567726314485 1103 6 0.6540727892090956 1103 7 0.6501785034835237 1103 8 0.732507272841886 1103 9 0.823673874887433 1103 10 0.9595865777736066 1103 11 0.7766670601394736 1103 12 0.8664112379161821 1103 13 0.915066712053239 1103 14 1.0766644972353632 1103 15 1.0842206467960978 1103 16 1.0675371192240988 1103 17 1.1936033554168333 1103 18 1.1360562551745588 1103 19 1.1459173149965878 1103 20 1.1670148920428787 1103 21 1.3383514963853358 1103 22 1.3504860755304833 1103 23 1.4090571777315333 1103 24 1.3175336420178638 1103 25 1.3851301594931087 1104 1 0.4434050899963822 1104 2 0.4671847069605448 1104 3 0.5259815247153732 1104 4 0.5188824693392462 1104 5 0.4440459301441706 1104 6 0.599490142295096 1104 7 0.5835193342383533 1104 8 0.6466597703950896 1104 9 0.6983579193613281 1104 10 0.7794904081454561 1104 11 0.8608440560239998 1104 12 0.8772281934362098 1104 13 0.6654870742394863 1104 14 0.9584755500557094 1104 15 0.8694705844864501 1104 16 1.0997606315431767 1104 17 1.0310712127550872 1104 18 1.098469857523483 1104 19 1.080810719899901 1104 20 1.0590295302517632 1104 21 1.000833791325796 1104 22 1.066386890723095 1104 23 1.1153750724555778 1104 24 1.2591388602232243 1104 25 1.3360848649356285 1105 1 0.42569746007569975 1105 2 0.45327623428590513 1105 3 0.5170204119909094 1105 4 0.46845776846092235 1105 5 0.5268599772875433 1105 6 0.5986451724026125 1105 7 0.6217365818874185 1105 8 0.5151990163017359 1105 9 0.6608351914789523 1105 10 0.6938971156552469 1105 11 0.7346712156539112 1105 12 0.7702943086856706 1105 13 0.6075095437908331 1105 14 0.8895711418670302 1105 15 0.6565008506198922 1105 16 0.9053948138117534 1105 17 1.0315388910530672 1105 18 0.8333324172515972 1105 19 0.8091607718576768 1105 20 0.9466654456124886 1105 21 0.9552328247128941 1105 22 0.7774550049765523 1105 23 0.9832816114379028 1105 24 1.0158516765912609 1105 25 1.1753784724380765 1106 1 0.1371892008044675 1106 2 0.2876181414648471 1106 3 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15 0.6806893870714248 1107 16 1.0452841073544477 1107 17 0.9597755765688305 1107 18 0.9580173416704199 1107 19 0.7900899214307984 1107 20 0.6390493303458555 1107 21 0.7347046519948961 1107 22 0.8403561168742257 1107 23 0.9035550512700539 1107 24 0.8378661702101482 1107 25 0.7680236289594902 1108 1 0.049470312742439404 1108 2 0.061435528347235646 1108 3 0.06695438906827533 1108 4 0.1261164548703846 1108 5 0.1107586541454566 1108 6 0.17616309388818507 1108 7 0.04153255982902948 1108 8 0.0865935923995238 1108 9 0.2675587402753976 1108 10 0.24178084958971624 1108 11 0.1855176837424679 1108 12 0.07495983727159938 1108 13 0.07681011704833907 1108 14 0.2624119787014081 1108 15 0.44409681114525057 1108 16 0.49620001962746585 1108 17 0.26342119882668413 1108 18 0.30967452278859753 1108 19 0.3458490883470873 1108 20 0.40338140902959907 1108 21 0.42654229121153264 1108 22 0.47370711560150625 1108 23 0.32752775891410835 1108 24 0.45745822159611294 1108 25 0.35474503575736804 1109 1 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-0.9632273164350948 2314 24 -1.0891144742061485 2314 25 -1.1777017157945053 2315 1 -0.6624992285392892 2315 2 -0.683431373238875 2315 3 -0.757627431044881 2315 4 -0.6653272888895216 2315 5 -0.8011493374988703 2315 6 -0.7709451225386701 2315 7 -0.9551027694915106 2315 8 -0.9470948756312865 2315 9 -0.9985277950025662 2315 10 -1.0820974543130077 2315 11 -0.9687618150027646 2315 12 -0.9044621529532719 2315 13 -0.9584796266918616 2315 14 -1.2586757323159787 2315 15 -1.204473177045962 2315 16 -1.1161456297726104 2315 17 -1.1210927068083407 2315 18 -1.108826604336464 2315 19 -1.290350579190644 2315 20 -1.1320241953711374 2315 21 -1.2874264354048535 2315 22 -1.4471519151694565 2315 23 -1.2252809305069334 2315 24 -1.3139315623633792 2315 25 -1.2987075905104035 2316 1 -0.9179685108537683 2316 2 -1.0121383740462875 2316 3 -0.9452727807981383 2316 4 -0.9783640019350557 2316 5 -0.9902057309272578 2316 6 -1.224364925188631 2316 7 -1.2369508655629318 2316 8 -1.9747450292354713 2316 9 -1.5854718945641295 2316 10 -1.5541815483144248 2316 11 -1.536814035523335 2316 12 -1.6501539179903446 2316 13 -1.8597464848122982 2316 14 -1.9023080738571707 2316 15 -1.865212059737459 2316 16 -1.7035834195530128 2316 17 -1.753133131984164 2316 18 -1.7398019191252487 2316 19 -1.6931440255055037 2316 20 -1.7082650209612353 2316 21 -1.78898033412203 2316 22 -1.948675702284632 2316 23 -1.9038759931845846 2316 24 -1.8982408967499182 2316 25 -1.9958198878785822 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24730.0 3 0.0 4 416.0 5 4166.0 6 5306.0 7 4175.0 8 3420.0 9 3139.0 10 2971.0 11 2851.0 12 2695.0 13 2719.0 14 2564.0 15 2598.0 16 2639.0 17 2828.0 18 2997.0 19 3285.0 20 4255.0 21 5317.0 22 6831.0 23 8765.0 24 11028.0 25 12777.0 26 14871.0 27 17207.0 28 19951.0 29 24736.0 30 31258.0 31 40781.0 32 52352.0 33 69175.0 34 90975.0 35 123357.0 36 184448.0 37 335084.0 38 844000.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.62252588700674 19.676876753847573 13.858293956955286 24.842303402190403 2 14.849490045756081 20.883538416180915 40.645426142519256 23.62154539554374 3 18.430815556357313 25.123118213275 30.276855501208473 26.169210729159214 4 12.6711412937853 17.718061955672876 38.24771003929126 31.363086711250553 5 13.277015399953097 37.71912369810041 35.86973846780162 13.13412243414487 6 31.242721373017357 37.60401934979375 18.243417076553268 12.909842200635621 7 27.468567748889082 31.8597205920635 22.666285069978844 18.005426589068573 8 24.817790118777044 37.0468983344218 20.825994447565215 17.309317099235944 9 26.212952264385613 15.558539316891185 20.467384900645314 37.76112351807789 10 15.005274863789772 29.312562700852045 34.410075370420266 21.27208706493791 11 34.115403566406705 22.72753336814388 24.600757002578316 18.5563060628711 12 22.94314564561136 24.90253300024814 31.887579180044117 20.266742174096386 13 28.77629756828526 21.952415096005566 26.0840111495245 23.187276186184675 14 20.926924741724502 22.107185029231218 27.97464005841677 28.99125017062751 15 22.930662562472502 30.45415587717255 23.906372816919347 22.7088087434356 16 22.27438730135533 28.184771957920844 27.491148935867905 22.049691804855918 17 21.639729086649343 28.322897780294692 28.003868740888237 22.03350439216773 18 21.524336683975527 27.58705555022741 29.622356288505365 21.266251477291696 19 23.18296292575052 27.12589189345536 27.86259677560948 21.82854840518464 20 23.207523138105017 27.358858701140278 28.253631892146164 21.17998626860855 21 22.96674171739822 26.83761386373243 27.666064332533097 22.52958008633625 22 22.977803961805826 27.64454877460271 27.856000024357236 21.52164723923423 23 22.480611894348463 27.387681429688527 27.9343491315377 22.197357544425316 24 22.882709255292752 26.891402758558396 28.471984358595336 21.753903627553512 25 22.815396770865632 27.219338472491668 28.154881126304854 21.81038363033785 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 320.0 1 320.0 2 491.5 3 663.0 4 663.0 5 663.0 6 1582.5 7 2502.0 8 2502.0 9 2502.0 10 3541.0 11 4580.0 12 4580.0 13 4580.0 14 7222.0 15 9864.0 16 9864.0 17 9864.0 18 17382.5 19 24901.0 20 24901.0 21 24901.0 22 39287.5 23 53674.0 24 53674.0 25 53674.0 26 75923.5 27 98173.0 28 98173.0 29 98173.0 30 125092.5 31 152012.0 32 152012.0 33 152012.0 34 170081.0 35 188150.0 36 188150.0 37 188150.0 38 202421.0 39 216692.0 40 216692.0 41 216692.0 42 229221.5 43 241751.0 44 241751.0 45 241751.0 46 247975.5 47 254200.0 48 254200.0 49 254200.0 50 245822.0 51 237444.0 52 237444.0 53 237444.0 54 213050.5 55 188657.0 56 188657.0 57 188657.0 58 161829.5 59 135002.0 60 135002.0 61 135002.0 62 109993.0 63 84984.0 64 84984.0 65 84984.0 66 65068.0 67 45152.0 68 45152.0 69 45152.0 70 32796.5 71 20441.0 72 20441.0 73 20441.0 74 14161.0 75 7881.0 76 7881.0 77 7881.0 78 5279.0 79 2677.0 80 2677.0 81 2677.0 82 1701.5 83 726.0 84 726.0 85 726.0 86 454.0 87 182.0 88 182.0 89 182.0 90 107.5 91 33.0 92 33.0 93 33.0 94 19.0 95 5.0 96 5.0 97 5.0 98 3.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.41589979433359364 2 0.0 3 0.0 4 0.0 5 0.03354194290562535 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0023342350584852744 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1970667.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.54696653543687 #Duplication Level Percentage of deduplicated Percentage of total 1 82.39887142798412 58.953892966157454 2 12.166804907272706 17.40995967087659 3 3.064015752140303 6.5766309744730105 4 1.0386285427233026 2.972428863558948 5 0.4677648428263288 1.6733577778074615 6 0.2425041426453709 1.0410261467131894 7 0.13632081337401772 0.6827338470788067 8 0.0902256950979679 0.5164299830248672 9 0.06294254988206449 0.4053013659060421 >10 0.292269866269743 3.6582241298767713 >50 0.02049189351196133 0.9830204915245141 >100 0.016038719227276423 2.2609003645634527 >500 0.001915065232092155 0.9429314389322118 >1k 0.0011348534708694195 1.5983299392179553 >5k 7.092834192933872E-5 0.32483204028873924 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6401 0.3248138828122661 No Hit TATCAACGCAGAGTACTTTTTTTTT 4194 0.2128213442453748 No Hit GGTATCAACGCAGAGTACTTTTTTT 3845 0.1951116043451278 No Hit GTCCTACAGTGGACATTTCTAAATT 2820 0.14309875793322768 No Hit CTGTAGGACGTGGAATATGGCAAGA 2685 0.13624828547897744 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2680 0.13599456427696816 No Hit CTTTAGGACGTGAAATATGGCGAGG 2507 0.12721581068744744 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1996 0.10128550384210017 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 5.0744240401853786E-5 0.0 4 0.0 0.0 0.0 1.0148848080370757E-4 0.0 5 0.0 0.0 0.0 1.0148848080370757E-4 0.0 6 0.0 0.0 0.0 1.0148848080370757E-4 0.0 7 0.0 0.0 0.0 1.0148848080370757E-4 0.0 8 0.0 0.0 0.0 1.0148848080370757E-4 0.0 9 0.0 0.0 0.0 1.0148848080370757E-4 0.0 10 0.0 0.0 0.0 1.0148848080370757E-4 0.0 11 0.0 0.0 0.0 2.0297696160741514E-4 0.0 12 0.0 0.0 0.0 2.0297696160741514E-4 1.5223272120556137E-4 13 0.0 0.0 0.0 2.0297696160741514E-4 1.5223272120556137E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTTTC 160 0.0 12.465553 13 TCCAACG 245 0.0 12.404982 18 GGTATCA 1215 0.0 12.241303 1 CGTGCGC 70 1.0938017E-4 12.211154 10 CCCGTTT 165 7.2759576E-12 11.512198 12 GGCGAGG 695 0.0 11.206043 19 TAGGACC 340 0.0 11.176441 4 AGGGCCG 60 0.0058767237 11.083303 5 GCACCGT 60 0.005887834 11.080491 6 TGGACCG 70 0.0014917427 10.857114 5 CGTTTCC 175 2.0008883E-11 10.854359 14 CTAGGAC 195 1.8189894E-12 10.71792 3 GCCGGTT 160 5.475158E-10 10.68476 11 TCCCGTT 185 6.002665E-11 10.267637 11 CGGTTTC 160 6.199116E-9 10.091162 13 CCGGTTT 160 6.199116E-9 10.091162 12 TGGCGAG 1485 0.0 9.97724 18 TAGACTG 200 2.5465852E-11 9.974974 5 ATCCCGT 210 7.2759576E-12 9.949829 10 GATATAC 595 0.0 9.934684 1 >>END_MODULE