FastQCFastQC Report
Thu 26 May 2016
SRR1511766_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511766_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2177338
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50050.2298678478031431No Hit
TATCAACGCAGAGTACTTTTTTTTT30520.14017116313590264No Hit
GGTATCAACGCAGAGTACTTTTTTT29820.13695622820159295No Hit
GTCCTACAGTGGACATTTCTAAATT22450.10310755610750376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10750.013.3370221
GTCAACG853.540307E-511.6326972
TAGGACC4000.011.1112334
GTATCAA22300.010.8189291
GTATTAG1904.5474735E-1110.4082021
TTAGGAC6700.010.3032073
AGGACCG1201.6169852E-610.2443985
ACTGTTC6850.010.2156388
TTATGCC1304.0935265E-710.1837814
TCCAACG1304.0935265E-710.18378118
GTCCTAC10700.09.9802021
AGGACGT11950.09.9707325
TCCTACA12200.09.968842
CTCCTAG2400.09.8877931
GGCGTGC1451.7298589E-79.782458
TAGGACG12500.09.7589714
GTATAAT2051.9826984E-109.6466261
GGCGAGG5600.09.62523219
AGGACCT9950.09.5989265
GTAGGAC13250.09.4920533
Produced by FastQC (version 0.11.5)