FastQCFastQC Report
Thu 26 May 2016
SRR1511766_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511766_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2177338
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61500.28245499780006594No Hit
TATCAACGCAGAGTACTTTTTTTTT40620.18655808147379965No Hit
GGTATCAACGCAGAGTACTTTTTTT39620.18196531728192866No Hit
GTCCTACAGTGGACATTTCTAAATT22700.10425574715547149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13200.013.0062941
TCCAACG2250.012.66294718
GTACTAA1302.4574547E-911.7390161
TATACCG658.023815E-411.6926345
GTCGTAG855.1129602E-511.2211171
GGCGAGG5550.010.95198919
ACCGGGC700.001495478210.8539548
TGTAGGA13500.010.274362
CCAACGA1856.002665E-1110.26748919
AGGACGT12600.010.2542535
CTAGTAC1303.8414692E-710.2310543
GTAGGAC13450.010.1006533
TAGGACC4050.010.08669954
TTAGGAC7900.09.981293
CTACACT4100.09.9636924
TACACCG1054.105008E-59.9526595
TAGGACG13650.09.9526584
TAGAACA8500.09.9473354
CTGTAGG13800.09.74529651
GTATCAA26500.09.6819181
Produced by FastQC (version 0.11.5)