##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511766_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2177338 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.71792757945712 31.0 31.0 34.0 27.0 34.0 2 30.903029295405673 31.0 31.0 34.0 27.0 34.0 3 31.001912427009493 31.0 31.0 34.0 27.0 34.0 4 34.58366776311257 37.0 35.0 37.0 31.0 37.0 5 34.18110647037805 37.0 35.0 37.0 30.0 37.0 6 34.13271435119398 37.0 35.0 37.0 30.0 37.0 7 33.942400766440485 37.0 35.0 37.0 30.0 37.0 8 33.844050395482924 37.0 35.0 37.0 29.0 37.0 9 35.31143993261497 39.0 35.0 39.0 30.0 39.0 10 35.09537012627346 38.0 35.0 39.0 29.0 39.0 11 35.09943747824178 38.0 35.0 39.0 29.0 39.0 12 34.89938172208449 38.0 35.0 39.0 27.0 39.0 13 34.87636646216619 38.0 35.0 39.0 27.0 39.0 14 35.85042790783976 39.0 36.0 41.0 27.0 41.0 15 35.76281863449772 39.0 36.0 41.0 27.0 41.0 16 35.5849394995173 39.0 36.0 41.0 27.0 41.0 17 35.54270535856169 39.0 36.0 41.0 27.0 41.0 18 35.44973357374923 39.0 35.0 40.0 27.0 41.0 19 35.46007050811588 39.0 35.0 40.0 27.0 41.0 20 35.56287999382732 39.0 36.0 41.0 27.0 41.0 21 35.50067835127114 39.0 36.0 41.0 27.0 41.0 22 35.39060954247802 39.0 36.0 41.0 27.0 41.0 23 35.26226153220125 39.0 35.0 41.0 26.0 41.0 24 35.082582033657616 39.0 35.0 41.0 25.0 41.0 25 34.52185558695986 38.0 34.0 40.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3748091577856094 1101 2 0.4421207929753166 1101 3 0.5113880127653516 1101 4 0.4965393584650144 1101 5 0.6287837450360172 1101 6 0.5188941751972038 1101 7 0.49314099020212865 1101 8 0.40543186979346757 1101 9 0.25251321726815945 1101 10 0.6926011398999137 1101 11 0.6178208422570464 1101 12 0.7547730252485465 1101 13 0.6352940343122526 1101 14 0.5221618289812682 1101 15 0.616988649043634 1101 16 0.5082666039339898 1101 17 0.4941689546196528 1101 18 0.6456383993374075 1101 19 0.48553879415104007 1101 20 0.22124531031261796 1101 21 0.4094852717944235 1101 22 0.31959857926165114 1101 23 0.38006711404948135 1101 24 0.021898352992266723 1101 25 0.3288674670735432 1102 1 0.722292523291312 1102 2 0.625859875794113 1102 3 0.6322529919440889 1102 4 0.540542879269573 1102 5 0.9036287294146277 1102 6 0.7532891240627464 1102 7 0.9302748751862779 1102 8 0.8863521302516659 1102 9 0.711368897688331 1102 10 0.9460780070229546 1102 11 0.9738941574925164 1102 12 1.0829181102838206 1102 13 1.0259341264574005 1102 14 1.2269095409666733 1102 15 0.9081093658701462 1102 16 0.9941325490100184 1102 17 1.165211934870321 1102 18 1.2537331996145369 1102 19 1.3696325718842743 1102 20 1.2070720385062117 1102 21 1.1637980395041154 1102 22 1.2959980046616977 1102 23 1.4056118292840765 1102 24 1.20681975219253 1102 25 1.2746326973855844 1103 1 0.3202477248614244 1103 2 0.4285996812796178 1103 3 0.5818362159248274 1103 4 0.5694633124199555 1103 5 0.601029655963913 1103 6 0.684627105356796 1103 7 0.5756800693394339 1103 8 0.5134017782313975 1103 9 0.6697435948291925 1103 10 0.8376996014027966 1103 11 0.6581627883219596 1103 12 0.7992722187083459 1103 13 0.6852874817753616 1103 14 0.8839360632498767 1103 15 0.9464745350718928 1103 16 0.900110237277147 1103 17 1.190276024999676 1103 18 1.1275160576919845 1103 19 1.1578018573078594 1103 20 0.9395594582033766 1103 21 1.2235972681404235 1103 22 1.3158046122209086 1103 23 1.241606709680866 1103 24 1.2359046896808792 1103 25 1.2423694029627512 1104 1 0.5149860730788625 1104 2 0.48320399822742743 1104 3 0.5424950744756636 1104 4 0.5685323590568174 1104 5 0.6063556940419517 1104 6 0.6262539138405501 1104 7 0.8106830796077915 1104 8 0.7467282362604521 1104 9 0.8370652735926782 1104 10 0.8080814409369168 1104 11 0.881969737210575 1104 12 0.9196685521271561 1104 13 0.9538359378050032 1104 14 1.1831957602161935 1104 15 1.2198658996897151 1104 16 1.2958100131793913 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2313 19 -0.45895282121724534 2313 20 -0.5095758115478688 2313 21 -0.5580261541626115 2313 22 -0.44669487745163394 2313 23 -0.6596841832508673 2313 24 -0.5164647999602536 2313 25 -0.4122115318315309 2314 1 -0.48431499145949175 2314 2 -0.6177856760334706 2314 3 -0.6042928429643055 2314 4 -0.7002855606084282 2314 5 -0.7499721571597391 2314 6 -0.6424049483016887 2314 7 -0.7782602813847248 2314 8 -0.8181809977169863 2314 9 -0.9044394870448684 2314 10 -2.1885638217871133 2314 11 -1.3146146554229219 2314 12 -1.255333722854239 2314 13 -1.0683418808587177 2314 14 -1.3267881581505918 2314 15 -1.123067090446419 2314 16 -1.1228435343004222 2314 17 -1.2913789487847964 2314 18 -1.1722517721360788 2314 19 -1.1272779575167604 2314 20 -0.9417930946560347 2314 21 -1.0723052125990336 2314 22 -1.1627482689945694 2314 23 -1.1196854368768498 2314 24 -1.1753667399141534 2314 25 -1.2188287384297354 2315 1 -0.7336861241432366 2315 2 -0.7692728609465895 2315 3 -0.7711476805813788 2315 4 -0.789886653247251 2315 5 -0.9065506028332777 2315 6 -1.1313898509700024 2315 7 -1.030823331018631 2315 8 -1.1102096246475384 2315 9 -1.0937716135034918 2315 10 -1.2142820595521826 2315 11 -1.1308057227005932 2315 12 -1.3484564079483263 2315 13 -1.3223778251661358 2315 14 -1.4476639079042997 2315 15 -1.622706447379187 2315 16 -1.467233470100794 2315 17 -1.7447859935180858 2315 18 -1.5194765301858695 2315 19 -1.4682579131170215 2315 20 -1.46458618811549 2315 21 -1.5535675794627082 2315 22 -1.713391200745285 2315 23 -1.3452670302740444 2315 24 -1.1427288649082712 2315 25 -1.5151873402185032 2316 1 -0.9426078298118057 2316 2 -1.132876932712282 2316 3 -1.1575177902690896 2316 4 -1.0407630037874611 2316 5 -1.1515570985822663 2316 6 -1.1966831463703542 2316 7 -1.32502945182312 2316 8 -2.120811673686333 2316 9 -1.6010790844370675 2316 10 -1.6899915762355704 2316 11 -1.5845569766268213 2316 12 -1.6326363813075346 2316 13 -1.9783629589456737 2316 14 -1.851702783728605 2316 15 -2.0358485809449434 2316 16 -2.0262424983696405 2316 17 -1.9730397059619662 2316 18 -1.8985519959886332 2316 19 -2.02276234502677 2316 20 -1.9722899819617936 2316 21 -1.8476030018508425 2316 22 -2.0464573587153225 2316 23 -1.8771060672587012 2316 24 -2.129643630814634 2316 25 -2.124414921662037 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 39156.0 3 1.0 4 674.0 5 6130.0 6 7646.0 7 6106.0 8 5100.0 9 4524.0 10 4293.0 11 3971.0 12 4007.0 13 3807.0 14 3697.0 15 3689.0 16 3819.0 17 4011.0 18 4281.0 19 4725.0 20 5721.0 21 7553.0 22 9449.0 23 11786.0 24 14433.0 25 17141.0 26 19451.0 27 22128.0 28 26092.0 29 32162.0 30 40261.0 31 51715.0 32 66475.0 33 86434.0 34 113280.0 35 153096.0 36 228329.0 37 408200.0 38 753995.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.04309951255954 19.444078082703474 13.919906662301068 24.59291574243591 2 15.629452110788494 20.743495038436844 39.573644514540234 24.05340833623443 3 19.00283740971774 24.793027081693335 29.992311712742808 26.211823795846122 4 13.336284949787307 17.44749781614063 37.316484624803316 31.89973260926875 5 13.715605434401091 37.4891346894221 35.3826427008084 13.41261717536841 6 32.266832251125 37.04270995132589 17.608428273423787 13.08202952412533 7 28.21762170136194 31.56101625011826 21.964297688278073 18.257064360241728 8 25.57425627073059 36.635010274013496 20.192684828905755 17.598048626350156 9 26.406327359371858 15.507743859703913 19.808913453033018 38.27701532789121 10 15.166960756667086 29.256642744488914 33.82795872758387 21.748437771260136 11 35.044490106726656 22.436663485412005 23.74656576057553 18.772280647285815 12 23.248480483967118 24.56237846397757 31.308184581355764 20.88095647069954 13 29.07977539545996 21.548468818346073 25.72650640369111 23.645249382502854 14 21.459093627172262 21.440952208614373 27.348854426827625 29.751099737385744 15 23.53116511997678 29.769930070572414 23.382543270727833 23.316361538722973 16 23.06660702196903 27.89070874618456 26.567533382506532 22.47515084933988 17 22.371767727380863 27.93415629543966 27.235918355349515 22.45815762182996 18 22.24565042267209 27.310504845825502 28.635746953389873 21.80809777811254 19 23.745922773588664 26.720793923589266 27.29034261102318 22.242940691798886 20 23.706103508045146 26.935964925978418 27.667638189385386 21.69029337659105 21 23.703301921888105 26.341569384266478 27.039899179640457 22.91522951420496 22 23.459747636793185 27.04159850239145 27.32396164490768 22.174692215907683 23 23.196077044537873 26.767088986643323 27.319231097790052 22.717602871028753 24 23.36173804893866 26.4653443792374 27.72872195313727 22.44419561868667 25 23.414712336644158 26.672138270207807 27.473221445623984 22.439927947524048 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 246.0 1 246.0 2 398.5 3 551.0 4 551.0 5 551.0 6 1378.5 7 2206.0 8 2206.0 9 2206.0 10 3228.0 11 4250.0 12 4250.0 13 4250.0 14 6808.0 15 9366.0 16 9366.0 17 9366.0 18 16864.5 19 24363.0 20 24363.0 21 24363.0 22 38268.0 23 52173.0 24 52173.0 25 52173.0 26 74131.0 27 96089.0 28 96089.0 29 96089.0 30 123499.0 31 150909.0 32 150909.0 33 150909.0 34 171685.5 35 192462.0 36 192462.0 37 192462.0 38 209882.5 39 227303.0 40 227303.0 41 227303.0 42 244806.5 43 262310.0 44 262310.0 45 262310.0 46 271463.0 47 280616.0 48 280616.0 49 280616.0 50 276500.0 51 272384.0 52 272384.0 53 272384.0 54 248613.0 55 224842.0 56 224842.0 57 224842.0 58 195386.0 59 165930.0 60 165930.0 61 165930.0 62 137251.0 63 108572.0 64 108572.0 65 108572.0 66 83811.5 67 59051.0 68 59051.0 69 59051.0 70 43294.0 71 27537.0 72 27537.0 73 27537.0 74 19230.5 75 10924.0 76 10924.0 77 10924.0 78 7366.0 79 3808.0 80 3808.0 81 3808.0 82 2464.0 83 1120.0 84 1120.0 85 1120.0 86 692.0 87 264.0 88 264.0 89 264.0 90 158.5 91 53.0 92 53.0 93 53.0 94 30.0 95 7.0 96 7.0 97 7.0 98 4.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40728632853511954 2 0.0 3 0.0 4 0.0 5 0.031506362356235 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0016533951090735568 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2177338.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.49349733086545 #Duplication Level Percentage of deduplicated Percentage of total 1 83.17035850587551 60.2931016235281 2 11.384399715327394 16.505899007531838 3 2.839620648041575 6.1756209560841695 4 1.079869642851733 3.1313410828701898 5 0.4963308333171822 1.7990378970152692 6 0.2763027096930171 1.2018089848584979 7 0.17266795643426666 0.8762112827225452 8 0.12624447459773191 0.7321522785829753 9 0.08258517529613554 0.5388199366409512 >10 0.3432787880552888 4.126604975686048 >50 0.014193405165250923 0.7263079193679457 >100 0.011549602353449524 1.6047213132110338 >500 0.0017746147258918068 0.8879011210948102 >1k 7.605491682385919E-4 1.1179054166800406 >5k 6.337909735321599E-5 0.28256620412569355 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6150 0.28245499780006594 No Hit TATCAACGCAGAGTACTTTTTTTTT 4062 0.18655808147379965 No Hit GGTATCAACGCAGAGTACTTTTTTT 3962 0.18196531728192866 No Hit GTCCTACAGTGGACATTTCTAAATT 2270 0.10425574715547149 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.592764191870991E-5 0.0 0.0 0.0 0.0 6 4.592764191870991E-5 0.0 0.0 0.0 0.0 7 4.592764191870991E-5 0.0 0.0 0.0 0.0 8 4.592764191870991E-5 0.0 0.0 0.0 4.592764191870991E-5 9 4.592764191870991E-5 0.0 0.0 4.592764191870991E-5 4.592764191870991E-5 10 4.592764191870991E-5 0.0 0.0 9.185528383741982E-5 4.592764191870991E-5 11 4.592764191870991E-5 0.0 0.0 1.3778292575612974E-4 4.592764191870991E-5 12 4.592764191870991E-5 0.0 0.0 1.8371056767483963E-4 4.592764191870991E-5 13 4.592764191870991E-5 0.0 0.0 1.8371056767483963E-4 4.592764191870991E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1320 0.0 13.006294 1 TCCAACG 225 0.0 12.662947 18 GTACTAA 130 2.4574547E-9 11.739016 1 TATACCG 65 8.023815E-4 11.692634 5 GTCGTAG 85 5.1129602E-5 11.221117 1 GGCGAGG 555 0.0 10.951989 19 ACCGGGC 70 0.0014954782 10.853954 8 TGTAGGA 1350 0.0 10.27436 2 CCAACGA 185 6.002665E-11 10.267489 19 AGGACGT 1260 0.0 10.254253 5 CTAGTAC 130 3.8414692E-7 10.231054 3 GTAGGAC 1345 0.0 10.100653 3 TAGGACC 405 0.0 10.0866995 4 TTAGGAC 790 0.0 9.98129 3 CTACACT 410 0.0 9.963692 4 TACACCG 105 4.105008E-5 9.952659 5 TAGGACG 1365 0.0 9.952658 4 TAGAACA 850 0.0 9.947335 4 CTGTAGG 1380 0.0 9.7452965 1 GTATCAA 2650 0.0 9.681918 1 >>END_MODULE