FastQCFastQC Report
Thu 26 May 2016
SRR1511765_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511765_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2315141
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51220.22123922473836366No Hit
GGTATCAACGCAGAGTACTTTTTTT30130.13014326125277034No Hit
TATCAACGCAGAGTACTTTTTTTTT29440.12716288122408095No Hit
GTCCTACAGTGGACATTTCTAAATT27790.12003588550330195No Hit
GTCCTAAAGTGTGTATTTCTCATTT24070.10396774969645478No Hit
CTGTAGGACGTGGAATATGGCAAGA23980.10357900447532137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGT903.8673534E-813.6594876
CCCGTTT1001.532353E-712.29353812
GCCGGTT1354.0199666E-1011.9082711
CCGGTTT1407.348717E-1011.48297512
TAGGACC6650.011.3763224
TGCGCCA1105.2748874E-711.17594412
TCCAACG2300.011.10118918
TGTACCG1201.3596036E-711.0326615
GTATCAA22800.010.8832021
GGTATCA10600.010.6320451
CCAACGA1252.3874236E-710.59135619
GTCTAGC1251.5440164E-610.2813741
GTCCTAC16850.010.2672931
GTGCGCC1401.0365511E-710.13203711
TGGCGAG16700.010.07945618
CCTACAC3300.010.0296933
CGAAATC1151.0842859E-59.867722513
TTAGGAC10300.09.8238133
TCCTACA19100.09.6789012
GACGTGA10000.09.64569957
Produced by FastQC (version 0.11.5)