##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511765_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2315141 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.09098754676281 31.0 29.0 34.0 22.0 34.0 2 26.431877799235554 31.0 25.0 34.0 10.0 34.0 3 28.10139123275861 31.0 27.0 34.0 16.0 34.0 4 32.56505759260451 35.0 32.0 37.0 25.0 37.0 5 33.004730165462924 35.0 33.0 37.0 26.0 37.0 6 33.253124107775726 37.0 35.0 37.0 27.0 37.0 7 33.28900615556461 37.0 35.0 37.0 27.0 37.0 8 33.31155294645121 37.0 35.0 37.0 27.0 37.0 9 34.872195689161046 39.0 35.0 39.0 28.0 39.0 10 34.74292451302102 39.0 35.0 39.0 27.0 39.0 11 34.75532289394037 39.0 35.0 39.0 27.0 39.0 12 34.650280911616186 39.0 35.0 39.0 27.0 39.0 13 34.63862157855612 39.0 35.0 39.0 27.0 39.0 14 35.75008563193343 40.0 36.0 41.0 27.0 41.0 15 35.67997111191068 40.0 36.0 41.0 27.0 41.0 16 35.61831180044758 40.0 36.0 41.0 27.0 41.0 17 35.5841691715537 40.0 36.0 41.0 27.0 41.0 18 35.51249362349853 40.0 36.0 41.0 27.0 41.0 19 35.53771541344566 40.0 36.0 41.0 26.0 41.0 20 35.46298475989151 40.0 36.0 41.0 26.0 41.0 21 35.37218899410446 40.0 36.0 41.0 25.0 41.0 22 35.25943387465386 40.0 36.0 41.0 25.0 41.0 23 35.14296882997623 40.0 36.0 41.0 25.0 41.0 24 35.01463582563654 40.0 35.0 41.0 24.0 41.0 25 34.90304521409279 39.0 35.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9941264984044977 1101 2 3.660977809437995 1101 3 2.1815845483837073 1101 4 1.170741876695999 1101 5 0.7425575269865092 1101 6 0.6371762617467667 1101 7 0.4296962614509994 1101 8 0.16832354154765028 1101 9 0.2887125900904266 1101 10 0.4412105076022499 1101 11 0.26542547614954515 1101 12 0.39630870569945387 1101 13 0.4946570392549745 1101 14 0.5375282741835861 1101 15 0.3393025552758999 1101 16 0.3620886057859636 1101 17 0.5327958050196813 1101 18 0.5659458469543424 1101 19 0.5234181493873464 1101 20 0.6608547559935829 1101 21 0.5951220974594946 1101 22 0.42071668860104694 1101 23 0.6179265652109152 1101 24 0.6546920304749975 1101 25 0.4655887241426484 1102 1 1.3922917673627992 1102 2 4.172407757236137 1102 3 2.6659629264744993 1102 4 1.6400981344134635 1102 5 1.1707709060035185 1102 6 1.0245534050433491 1102 7 0.9551703610016276 1102 8 0.9381812137404992 1102 9 1.0761512070822263 1102 10 1.0562799786818005 1102 11 0.9861854477294472 1102 12 0.9207521172525404 1102 13 1.058965972867739 1102 14 1.1600089288324895 1102 15 1.1553300680192393 1102 16 1.2307426317326033 1102 17 1.2634834639939925 1102 18 1.2099077129494233 1102 19 1.032059774188859 1102 20 1.2295558424480433 1102 21 1.1698909907056532 1102 22 1.1106176874155835 1102 23 1.1279045536619847 1102 24 0.9398083347402846 1102 25 0.9758358716132562 1103 1 1.5314597438444117 1103 2 4.032959021505697 1103 3 2.684285962143008 1103 4 1.645500084517451 1103 5 1.0756951626075235 1103 6 0.9327894361453133 1103 7 0.8951440336026977 1103 8 0.7301421642752217 1103 9 0.9993513507551413 1103 10 0.893353135775719 1103 11 1.0002916453299378 1103 12 1.1840794152492222 1103 13 1.0880680313424236 1103 14 1.1856238863584707 1103 15 0.919520967249646 1103 16 0.8656193748684586 1103 17 1.0554065061069338 1103 18 1.0263546608239196 1103 19 0.8969193305939882 1103 20 1.0194865600686853 1103 21 1.1572186791590795 1103 22 1.1147864291734635 1103 23 1.0178447957735983 1103 24 1.0881281150230677 1103 25 0.9773176170496782 1104 1 1.273837078770427 1104 2 3.758262606565175 1104 3 2.304535377579139 1104 4 1.407811440161538 1104 5 0.9195335714190875 1104 6 0.6965122594175028 1104 7 0.5926326001457696 1104 8 0.596226213529981 1104 9 0.6571756280438734 1104 10 0.6248799271387213 1104 11 0.6859090861524493 1104 12 0.6683000039632603 1104 13 0.5362342730890433 1104 14 0.6654885038473353 1104 15 0.73837503310736 1104 16 0.3833131460390504 1104 17 0.693098246585393 1104 18 0.6685323641429903 1104 19 0.8538320046854295 1104 20 0.882300557547623 1104 21 0.8401254191674283 1104 22 0.9335082268550607 1104 23 0.6960256234445552 1104 24 0.7014292395757522 1104 25 0.7295784125573874 1105 1 1.5644225619727052 1105 2 4.01390199918033 1105 3 2.587990113755289 1105 4 1.6562761273197282 1105 5 1.2078286910851403 1105 6 1.0797653470663064 1105 7 0.9511099404366021 1105 8 0.9407803673000217 1105 9 0.9994674310032252 1105 10 0.8932388210066904 1105 11 0.9641103588783153 1105 12 0.9164535343855107 1105 13 1.0173970080619341 1105 14 1.0367094794314369 1105 15 1.0986559619576255 1105 16 1.040302493445978 1105 17 1.0182462615174686 1105 18 1.05983630911048 1105 19 1.1072719673810951 1105 20 1.1512080698710676 1105 21 1.0622286232198803 1105 22 1.1390235877408514 1105 23 1.2324546646249885 1105 24 1.2344532262261794 1105 25 1.1860084966731321 1106 1 1.123735882171978 1106 2 3.6725091878714835 1106 3 2.1398411911765365 1106 4 1.3479943806561536 1106 5 0.7492638012251547 1106 6 0.6022935510828731 1106 7 0.5387265499694749 1106 8 0.5721767466735344 1106 9 0.5841312751900603 1106 10 0.530406928647146 1106 11 0.6158519005508154 1106 12 0.6052204611116636 1106 13 0.6628057145233939 1106 14 0.8655733002148409 1106 15 0.8780176812276679 1106 16 0.6214462308210713 1106 17 0.5219879336353443 1106 18 0.6435664137928967 1106 19 0.628336497557143 1106 20 0.6855967318659992 1106 21 0.5019882776163698 1106 22 0.5484278360397852 1106 23 0.5506406682199056 1106 24 0.6304268762604224 1106 25 0.6713895026755381 1107 1 1.5731062914059777 1107 2 3.9643068539820874 1107 3 2.6097085089889553 1107 4 1.7198744450037395 1107 5 1.2715851146776487 1107 6 1.070818971752253 1107 7 0.9861818051877975 1107 8 1.0338131669323118 1107 9 1.1137278927761542 1107 10 1.0579143323885916 1107 11 1.1115747893687384 1107 12 1.1366449548504676 1107 13 1.1417563767441834 1107 14 1.202123682353033 1107 15 1.3236847320491734 1107 16 1.3169074844088797 1107 17 1.2676890029875238 1107 18 1.1545460681328876 1107 19 1.2250150789744936 1107 20 1.4159542537300922 1107 21 1.4467275052469546 1107 22 1.4441144252010645 1107 23 1.546521252267496 1107 24 1.4571406465252537 1107 25 1.4642697118250396 1108 1 1.4530448339871889 1108 2 4.023199259518066 1108 3 2.5168991763571107 1108 4 1.4709304072695844 1108 5 1.1297401307681127 1108 6 0.9414480348212066 1108 7 0.8221524855345379 1108 8 0.7749235828279311 1108 9 0.8506663241597678 1108 10 0.7696362188973325 1108 11 0.8759657501657756 1108 12 0.8964112573311667 1108 13 0.8424933512732906 1108 14 0.8370735853455713 1108 15 0.8156440669215641 1108 16 0.9086650039389994 1108 17 0.9794947026112766 1108 18 0.9068480243807784 1108 19 0.7993568970718172 1108 20 0.7837788967775055 1108 21 0.8356045920690391 1108 22 0.8767473519716376 1108 23 0.9409901675598249 1108 24 1.0261528633240573 1108 25 1.0139273228027292 1109 1 0.9682126743847022 1109 2 3.015230716750054 1109 3 1.8897342500499725 1109 4 1.10317332285436 1109 5 0.8645119537558017 1109 6 0.5962945780749038 1109 7 0.6081409412534384 1109 8 0.5762360443002876 1109 9 0.49489549121560117 1109 10 0.4745068594368149 1109 11 0.44928986799963155 1109 12 0.5613125331797093 1109 13 0.31289702751038106 1109 14 0.3674384070443395 1109 15 0.4438210792317321 1109 16 0.37512531813771943 1109 17 0.5488433286923851 1109 18 0.3534902824272166 1109 19 0.47128262512741514 1109 20 0.45154569482730267 1109 21 0.5350587605258781 1109 22 0.5900714698054941 1109 23 0.6726584842990349 1109 24 0.627766073867086 1109 25 0.5707737539760274 1110 1 0.8260598779038695 1110 2 3.490716846564222 1110 3 1.8637049835089883 1110 4 1.0718617149900993 1110 5 0.4317424053223675 1110 6 0.39666265420381563 1110 7 0.25400143142311293 1110 8 0.3077397699489097 1110 9 0.13961584313229025 1110 10 0.21113312750397029 1110 11 0.22519614324875903 1110 12 0.1558560290051858 1110 13 0.21050363492576452 1110 14 0.14713496867464926 1110 15 0.2707534789085386 1110 16 0.28703302978149026 1110 17 0.3431677743338284 1110 18 0.32381975544993225 1110 19 0.3653574971985165 1110 20 0.26115337953867623 1110 21 0.2143082443215505 1110 22 0.2234460994850025 1110 23 0.33505187688364657 1110 24 0.34090190293612466 1110 25 0.1736214063685182 1111 1 0.3616322545550581 1111 2 2.888812811621573 1111 3 1.43533316661863 1111 4 0.5140208227652678 1111 5 0.005441067409172717 1111 6 -0.15137818289306182 1111 7 -0.551277070792608 1111 8 -0.3872223825859109 1111 9 -0.6324597245796824 1111 10 -0.6204562540027254 1111 11 -0.7578265652303458 1111 12 -0.7382145347842197 1111 13 -0.8965993907511418 1111 14 -0.9423716360790806 1111 15 -1.0029642659338123 1111 16 -0.9897740011537763 1111 17 -0.8374452061805613 1111 18 -0.9989830772791137 1111 19 -0.924814883693287 1111 20 -1.0662250739099974 1111 21 -0.9529393862363023 1111 22 -0.8211153151832775 1111 23 -1.0276943436706034 1111 24 -0.9694907777669712 1111 25 -1.1052635246155162 1112 1 0.7055539564623388 1112 2 3.192865280083044 1112 3 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-2.3087907757786894 2315 21 -2.39477134370604 2315 22 -2.234779503453467 2315 23 -2.2280487228741777 2315 24 -2.150402282197007 2315 25 -2.081882310872686 2316 1 -0.8008403378075037 2316 2 1.6721348525377664 2316 3 0.180827962696398 2316 4 -0.7927859169256983 2316 5 -1.045154254707029 2316 6 -1.3016614343563688 2316 7 -1.7766325466738877 2316 8 -1.4787044888099174 2316 9 -1.60602370553314 2316 10 -1.7295921723585437 2316 11 -1.6711255037431343 2316 12 -1.8659011890292305 2316 13 -1.9433323533167766 2316 14 -2.077283084707993 2316 15 -2.0919367500773802 2316 16 -2.275959728479542 2316 17 -2.137895256055401 2316 18 -2.1423126699992707 2316 19 -2.0093562704021153 2316 20 -1.9482863635040388 2316 21 -2.0971217917093554 2316 22 -1.9909197446338993 2316 23 -2.2207329213906633 2316 24 -2.1609331252722868 2316 25 -1.9914283139178295 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 91154.0 3 5.0 4 804.0 5 4092.0 6 4767.0 7 4296.0 8 3921.0 9 3728.0 10 3720.0 11 3591.0 12 3415.0 13 3429.0 14 3591.0 15 3594.0 16 3812.0 17 4230.0 18 4653.0 19 5903.0 20 7602.0 21 9604.0 22 11927.0 23 14385.0 24 16408.0 25 18953.0 26 20989.0 27 23883.0 28 28189.0 29 33605.0 30 42215.0 31 53631.0 32 68892.0 33 90277.0 34 123387.0 35 174547.0 36 270863.0 37 346928.0 38 806151.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.187719020137436 18.11755741874901 12.812869712903016 24.881853848210543 2 16.663882007519483 20.620370880473097 39.38977129970729 23.325975812300126 3 19.00627218817342 24.173214503997812 29.129759267362115 27.690754040466647 4 12.652447518315299 16.4690617115761 37.808971462213314 33.069519307895284 5 13.712296572865323 37.40994608967661 35.3032493485278 13.574507988930264 6 32.37725045688362 36.63824363181335 17.861590287589397 13.122915623713633 7 28.521113832807593 31.28336459852769 21.847222264216303 18.34829930444841 8 25.413354953326817 36.802423696872026 20.202095682293216 17.58212566750794 9 26.336063332643672 15.824651716677298 19.968373416565125 37.8709115341139 10 15.25453525292844 28.862432136962717 33.89240655320778 21.990626056901068 11 34.79472740537186 22.444594087357963 24.009725541554488 18.750952965715694 12 23.53359903349299 24.304178449606308 31.355152882696995 20.807069634203703 13 29.09900520097912 21.63846608046767 25.628849387575098 23.633679330978115 14 21.395500317259295 21.62434167076649 27.351638625897944 29.62851938607627 15 23.366956915367144 30.11211844116622 23.330889997628653 23.190034645837986 16 22.772565472254175 27.791482246653658 26.974944506619686 22.46100777447248 17 22.241971439320544 28.051250442197688 27.358981591186023 22.347796527295746 18 22.0632350254261 27.335786459658394 28.982899961600612 21.618078553314895 19 23.654023664217426 26.665028177549445 27.2565688223741 22.424379335859026 20 23.716870808300662 26.881991204855343 27.81156741641222 21.58957057043178 21 23.45358575976295 26.43704673171701 26.97045090340499 23.13891660511505 22 23.44048985840031 27.335539700352207 27.216323314837513 22.00764712640997 23 23.015547344805153 27.02207486257744 27.356139330886236 22.606238461731174 24 23.26667069810033 26.441338320792696 28.02107000008639 22.270920981020588 25 23.214093655634795 26.789469842225593 27.64479571654599 22.35164078559362 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 217.0 1 217.0 2 362.0 3 507.0 4 507.0 5 507.0 6 1321.0 7 2135.0 8 2135.0 9 2135.0 10 3242.0 11 4349.0 12 4349.0 13 4349.0 14 7008.0 15 9667.0 16 9667.0 17 9667.0 18 17423.5 19 25180.0 20 25180.0 21 25180.0 22 39387.5 23 53595.0 24 53595.0 25 53595.0 26 76772.5 27 99950.0 28 99950.0 29 99950.0 30 129311.0 31 158672.0 32 158672.0 33 158672.0 34 181587.5 35 204503.0 36 204503.0 37 204503.0 38 224900.0 39 245297.0 40 245297.0 41 245297.0 42 262965.0 43 280633.0 44 280633.0 45 280633.0 46 291224.0 47 301815.0 48 301815.0 49 301815.0 50 296784.5 51 291754.0 52 291754.0 53 291754.0 54 264787.5 55 237821.0 56 237821.0 57 237821.0 58 206771.5 59 175722.0 60 175722.0 61 175722.0 62 144851.0 63 113980.0 64 113980.0 65 113980.0 66 88353.0 67 62726.0 68 62726.0 69 62726.0 70 46041.0 71 29356.0 72 29356.0 73 29356.0 74 20517.5 75 11679.0 76 11679.0 77 11679.0 78 7834.5 79 3990.0 80 3990.0 81 3990.0 82 2565.0 83 1140.0 84 1140.0 85 1140.0 86 733.5 87 327.0 88 327.0 89 327.0 90 208.0 91 89.0 92 89.0 93 89.0 94 58.0 95 27.0 96 27.0 97 27.0 98 18.5 99 10.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.346473929665623 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 4.7513304805193285E-4 22 9.934600095631325E-4 23 8.206843557260658E-4 24 4.3193913459266626E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2315141.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.61991421816818 #Duplication Level Percentage of deduplicated Percentage of total 1 84.16014156700471 62.800225443187806 2 10.997364460346349 16.41244785313952 3 2.6457577600061297 5.922786512811305 4 0.9402254900269484 2.8063818164618413 5 0.4203775080793319 1.5684266796063522 6 0.23345182413046814 1.0452093054414249 7 0.1482592354103637 0.7744163999860778 8 0.09600155886042609 0.5730902469580335 9 0.06112830860823305 0.41052502301832555 >10 0.2697370006504035 3.352442627700974 >50 0.014496500794056929 0.752945063874257 >100 0.010895645624999723 1.6003430309411943 >500 0.0014031181347371369 0.7512264810630052 >1k 7.015590673671171E-4 1.0060850816636586 >5k 5.846325561392642E-5 0.2234484341462692 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5122 0.22123922473836366 No Hit GGTATCAACGCAGAGTACTTTTTTT 3013 0.13014326125277034 No Hit TATCAACGCAGAGTACTTTTTTTTT 2944 0.12716288122408095 No Hit GTCCTACAGTGGACATTTCTAAATT 2779 0.12003588550330195 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2407 0.10396774969645478 No Hit CTGTAGGACGTGGAATATGGCAAGA 2398 0.10357900447532137 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.3193913459266626E-5 2 0.0 0.0 0.0 0.0 4.3193913459266626E-5 3 0.0 0.0 0.0 0.0 4.3193913459266626E-5 4 0.0 0.0 0.0 0.0 4.3193913459266626E-5 5 0.0 0.0 0.0 0.0 4.3193913459266626E-5 6 0.0 0.0 0.0 0.0 4.3193913459266626E-5 7 0.0 0.0 0.0 0.0 4.3193913459266626E-5 8 0.0 0.0 0.0 0.0 4.3193913459266626E-5 9 0.0 0.0 0.0 0.0 4.3193913459266626E-5 10 0.0 0.0 0.0 0.0 4.3193913459266626E-5 11 0.0 0.0 0.0 0.0 4.3193913459266626E-5 12 0.0 0.0 0.0 0.0 4.3193913459266626E-5 13 0.0 0.0 0.0 0.0 1.2958174037779987E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCACCGT 90 3.8673534E-8 13.659487 6 CCCGTTT 100 1.532353E-7 12.293538 12 GCCGGTT 135 4.0199666E-10 11.90827 11 CCGGTTT 140 7.348717E-10 11.482975 12 TAGGACC 665 0.0 11.376322 4 TGCGCCA 110 5.2748874E-7 11.175944 12 TCCAACG 230 0.0 11.101189 18 TGTACCG 120 1.3596036E-7 11.032661 5 GTATCAA 2280 0.0 10.883202 1 GGTATCA 1060 0.0 10.632045 1 CCAACGA 125 2.3874236E-7 10.591356 19 GTCTAGC 125 1.5440164E-6 10.281374 1 GTCCTAC 1685 0.0 10.267293 1 GTGCGCC 140 1.0365511E-7 10.132037 11 TGGCGAG 1670 0.0 10.079456 18 CCTACAC 330 0.0 10.029693 3 CGAAATC 115 1.0842859E-5 9.8677225 13 TTAGGAC 1030 0.0 9.823813 3 TCCTACA 1910 0.0 9.678901 2 GACGTGA 1000 0.0 9.6456995 7 >>END_MODULE