FastQCFastQC Report
Thu 26 May 2016
SRR1511765_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511765_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2315141
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68830.2973037063401322No Hit
TATCAACGCAGAGTACTTTTTTTTT42420.18322858089420904No Hit
GGTATCAACGCAGAGTACTTTTTTT41540.17942751650979358No Hit
GTCCTACAGTGGACATTTCTAAATT30950.13368516215643023No Hit
GTCCTAAAGTGTGTATTTCTCATTT27180.1174010567822867No Hit
CTGTAGGACGTGGAATATGGCAAGA26620.11498219762856776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5600.014.2512154
CGACGGC400.00529078114.24567412
ATCCCGT1251.4188117E-912.15630910
CTCCGCG550.003076210912.08723915
TCCAACG1750.011.93923318
GGGTCGT802.8767043E-511.8713957
GGCGAGG6600.011.51190619
GGTATCA13850.011.2929991
TAGGACT3650.010.9324394
TGGCGAG16350.010.629818
GTAGGAC18300.010.5910673
GTCCTAA8300.010.4563121
TGTAGGA19250.010.3645212
GACCGCT750.002655085210.1302597
TTAGGAC11550.010.1177463
GTATCAA28850.010.0494671
GTCCTAC16750.010.0210481
CGGTTTC951.6496565E-49.99696513
CTGTAGG20000.09.8231891
GTCTTAT1951.5279511E-109.7816151
Produced by FastQC (version 0.11.5)