##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511765_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2315141 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.306601196212238 33.0 31.0 34.0 28.0 34.0 2 31.487263194768698 33.0 31.0 34.0 28.0 34.0 3 31.599430013117992 34.0 31.0 34.0 28.0 34.0 4 35.13413740243035 37.0 35.0 37.0 32.0 37.0 5 34.81245202775987 37.0 35.0 37.0 32.0 37.0 6 34.777902080262066 37.0 35.0 37.0 32.0 37.0 7 34.62640936340378 37.0 35.0 37.0 31.0 37.0 8 34.547066463770456 37.0 35.0 37.0 31.0 37.0 9 36.10968921547327 39.0 37.0 39.0 32.0 39.0 10 35.943952441773526 39.0 37.0 39.0 31.0 39.0 11 35.940922388744355 39.0 37.0 39.0 30.0 39.0 12 35.78416433383539 39.0 35.0 39.0 30.0 39.0 13 35.766190914505856 39.0 35.0 39.0 30.0 39.0 14 36.88054464069359 40.0 37.0 41.0 31.0 41.0 15 36.80636946086653 40.0 37.0 41.0 30.0 41.0 16 36.657811770427806 40.0 36.0 41.0 30.0 41.0 17 36.639502302451554 40.0 36.0 41.0 30.0 41.0 18 36.57185717846127 40.0 36.0 41.0 30.0 41.0 19 36.5842266194586 40.0 37.0 41.0 30.0 41.0 20 36.68654522553918 40.0 37.0 41.0 30.0 41.0 21 36.63379984199666 40.0 37.0 41.0 30.0 41.0 22 36.547825380829934 40.0 37.0 41.0 30.0 41.0 23 36.45077254473917 40.0 37.0 41.0 30.0 41.0 24 36.31027786212589 40.0 37.0 41.0 30.0 41.0 25 35.81810308745774 39.0 36.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1466609528826872 1101 2 0.25945616232319324 1101 3 0.28264661035030514 1101 4 0.22498597251571795 1101 5 0.27059413094870877 1101 6 0.26750793942355955 1101 7 0.23325856780843424 1101 8 0.14099042569099396 1101 9 0.15874830325081035 1101 10 0.39151197463394993 1101 11 0.3381140842735135 1101 12 0.34274458111651995 1101 13 0.2875304874292084 1101 14 0.09646312325782702 1101 15 0.23469157723354073 1101 16 0.23931842719468932 1101 17 0.1635262411449787 1101 18 0.339449174140519 1101 19 0.15524007449088373 1101 20 0.06333110177597945 1101 21 0.32029877844201593 1101 22 0.3311065570234959 1101 23 0.2514100455830999 1101 24 0.2609686486322431 1101 25 0.40144604561077557 1102 1 0.35345415895112353 1102 2 0.44481918359812056 1102 3 0.3079475581866582 1102 4 0.37726081362000485 1102 5 0.4574168501948108 1102 6 0.38580344942548805 1102 7 0.5451933157300104 1102 8 0.5434569186577889 1102 9 0.40059844382775367 1102 10 0.49907207835085643 1102 11 0.5072613024074286 1102 12 0.478742435582987 1102 13 0.6259855001471522 1102 14 0.6437954502475804 1102 15 0.5231693463429892 1102 16 0.5209823377826268 1102 17 0.6875532179886008 1102 18 0.80993236899279 1102 19 0.8291477556073374 1102 20 0.7406234636088911 1102 21 0.7165112539746588 1102 22 0.7757340414241227 1102 23 0.8052563594104285 1102 24 0.6578843716301321 1102 25 0.7817346092302486 1103 1 0.33485083788906067 1103 2 0.36870736512639013 1103 3 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22 -0.7686724141103198 2314 23 -0.6808368083913621 2314 24 -0.8157967281021854 2314 25 -0.8143681622736878 2315 1 -0.6610250810023537 2315 2 -0.7028168457150947 2315 3 -0.6118379572471291 2315 4 -0.5428773488071812 2315 5 -0.7773616279645736 2315 6 -0.7343391689558132 2315 7 -0.6307127833919921 2315 8 -0.7350450870437442 2315 9 -0.8295232141631459 2315 10 -0.997596921889695 2315 11 -0.9633165199608342 2315 12 -0.9528186348346637 2315 13 -0.9809516545381598 2315 14 -1.162178905040257 2315 15 -1.049807345883913 2315 16 -1.1194167694948192 2315 17 -1.117467284622137 2315 18 -1.0719120896627459 2315 19 -1.0097181553341557 2315 20 -1.1761057198618872 2315 21 -1.2503129492363456 2315 22 -1.2817923261621544 2315 23 -1.186820919366248 2315 24 -1.3088058048360978 2315 25 -1.3555129548771774 2316 1 -0.9395558971735198 2316 2 -1.0165076018601944 2316 3 -1.1080615413932335 2316 4 -1.0128695466141977 2316 5 -1.093953668930574 2316 6 -1.1774187832106051 2316 7 -1.2176562917282823 2316 8 -1.9047852580741917 2316 9 -1.4083010748675946 2316 10 -1.4975745017949151 2316 11 -1.4671159786899253 2316 12 -1.4761142343181035 2316 13 -1.7152118761084836 2316 14 -1.7437199178705285 2316 15 -1.7756630212568822 2316 16 -1.8123580019423713 2316 17 -1.6057872871331895 2316 18 -1.6560310397247235 2316 19 -1.5380286317960454 2316 20 -1.6482957097379725 2316 21 -1.6562555448256617 2316 22 -1.7265746220595801 2316 23 -1.683551446766593 2316 24 -1.8650158472449476 2316 25 -1.6885973421210423 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26627.0 3 1.0 4 419.0 5 4366.0 6 5743.0 7 4614.0 8 3850.0 9 3488.0 10 3222.0 11 3216.0 12 2978.0 13 2879.0 14 2907.0 15 2784.0 16 2990.0 17 3090.0 18 3401.0 19 3716.0 20 4687.0 21 6039.0 22 7790.0 23 9807.0 24 11990.0 25 14618.0 26 16890.0 27 19412.0 28 22646.0 29 27925.0 30 35572.0 31 46060.0 32 59811.0 33 78620.0 34 104687.0 35 141329.0 36 209005.0 37 379352.0 38 1038610.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.57323142174645 18.934396114855705 13.574061213751904 24.91831124964594 2 15.15432537370294 20.09834390216406 40.56785310268359 24.179477621449404 3 18.975950060925015 24.31221251750973 29.556039999291624 27.15579742227363 4 12.846949710622377 16.89473772871717 37.58099398697531 32.67731857368514 5 13.621539222006565 37.406216135404804 35.45077610954889 13.521468533039746 6 32.43776512964005 36.8183622509385 17.69278847379058 13.05108414563087 7 28.52526044849968 31.30021022477681 21.805324168160816 18.369205158562696 8 25.40082871842363 36.93403555118241 20.05990131918531 17.60523441120865 9 26.254297254465282 15.61796884077471 19.94746756245084 38.180266342309174 10 15.06901739462089 29.032659349905686 33.93469339448439 21.963629860989027 11 34.91169652301955 22.443946178656073 23.927095585106912 18.717261713217468 12 23.449198126593586 24.3435281047677 31.37135060024422 20.835923168394498 13 29.05913721885622 21.61008767932493 25.739814551251953 23.590960550566898 14 21.299005114591292 21.597906995729417 27.3966466837225 29.706441205956786 15 23.276724830150734 30.1602796546733 23.36471083186726 23.198284683308707 16 22.80284440558912 27.844135627160504 26.92315500438202 22.42986496286835 17 22.086214187386428 28.166103058085877 27.455390406027107 22.292292348500588 18 22.0110567779673 27.249873765787914 29.05654558404866 21.68252387219612 19 23.552258804107396 26.67725205505842 27.447097174642927 22.32339196619126 20 23.6532029798617 26.852230598481906 27.940328472434295 21.5542379492221 21 23.382204366818264 26.33498348480719 27.189359093031484 23.093453055343065 22 23.380217446799136 27.23432395694258 27.364596799935725 22.020861796322556 23 22.899728353478256 26.971445799629485 27.53871146509003 22.59011438180223 24 23.18459221274212 26.43009648224449 28.103903822704535 22.281407482308854 25 23.107558018991803 26.790027575435055 27.779831921157754 22.32258248441539 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 218.0 1 218.0 2 392.5 3 567.0 4 567.0 5 567.0 6 1479.0 7 2391.0 8 2391.0 9 2391.0 10 3494.0 11 4597.0 12 4597.0 13 4597.0 14 7269.5 15 9942.0 16 9942.0 17 9942.0 18 17769.5 19 25597.0 20 25597.0 21 25597.0 22 40305.5 23 55014.0 24 55014.0 25 55014.0 26 78588.5 27 102163.0 28 102163.0 29 102163.0 30 131630.5 31 161098.0 32 161098.0 33 161098.0 34 183154.5 35 205211.0 36 205211.0 37 205211.0 38 224990.5 39 244770.0 40 244770.0 41 244770.0 42 262338.5 43 279907.0 44 279907.0 45 279907.0 46 290285.5 47 300664.0 48 300664.0 49 300664.0 50 296095.5 51 291527.0 52 291527.0 53 291527.0 54 264582.0 55 237637.0 56 237637.0 57 237637.0 58 205751.0 59 173865.0 60 173865.0 61 173865.0 62 143383.0 63 112901.0 64 112901.0 65 112901.0 66 87509.5 67 62118.0 68 62118.0 69 62118.0 70 45414.5 71 28711.0 72 28711.0 73 28711.0 74 19811.5 75 10912.0 76 10912.0 77 10912.0 78 7363.0 79 3814.0 80 3814.0 81 3814.0 82 2467.0 83 1120.0 84 1120.0 85 1120.0 86 720.5 87 321.0 88 321.0 89 321.0 90 191.0 91 61.0 92 61.0 93 61.0 94 37.0 95 13.0 96 13.0 97 13.0 98 7.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.420881492747094 2 0.0 3 0.0 4 0.0 5 0.03356167075785017 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.001598174797992865 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2315141.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.60704177725768 #Duplication Level Percentage of deduplicated Percentage of total 1 82.33187131249615 58.95541748673714 2 11.93970731428494 17.099342409244617 3 3.1095998089763843 6.680077302957735 4 1.1157171214945079 3.1957281012183563 5 0.504626787066605 1.8067415711700854 6 0.2827166162420245 1.2146700330220543 7 0.16794095266629408 0.8418028369581443 8 0.11575200398332997 0.6630926868028485 9 0.07468118482989979 0.4812928849880763 >10 0.3252026369711244 3.8929287391332306 >50 0.016789365053153513 0.8292310487712753 >100 0.012681730882200867 1.7630243366557699 >500 0.0016278990321309803 0.8128315567306014 >1k 0.0010249734646729425 1.4666543163356969 >5k 6.0292556745467204E-5 0.2971646892743399 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6883 0.2973037063401322 No Hit TATCAACGCAGAGTACTTTTTTTTT 4242 0.18322858089420904 No Hit GGTATCAACGCAGAGTACTTTTTTT 4154 0.17942751650979358 No Hit GTCCTACAGTGGACATTTCTAAATT 3095 0.13368516215643023 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2718 0.1174010567822867 No Hit CTGTAGGACGTGGAATATGGCAAGA 2662 0.11498219762856776 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.3193913459266626E-5 0.0 4 0.0 0.0 0.0 4.3193913459266626E-5 0.0 5 0.0 0.0 0.0 4.3193913459266626E-5 0.0 6 0.0 0.0 0.0 4.3193913459266626E-5 4.3193913459266626E-5 7 0.0 0.0 0.0 4.3193913459266626E-5 4.3193913459266626E-5 8 0.0 0.0 0.0 4.3193913459266626E-5 4.3193913459266626E-5 9 0.0 0.0 0.0 4.3193913459266626E-5 4.3193913459266626E-5 10 0.0 0.0 0.0 4.3193913459266626E-5 4.3193913459266626E-5 11 0.0 0.0 0.0 4.3193913459266626E-5 4.3193913459266626E-5 12 0.0 0.0 0.0 4.3193913459266626E-5 4.3193913459266626E-5 13 0.0 0.0 0.0 4.3193913459266626E-5 4.3193913459266626E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 560 0.0 14.251215 4 CGACGGC 40 0.005290781 14.245674 12 ATCCCGT 125 1.4188117E-9 12.156309 10 CTCCGCG 55 0.0030762109 12.087239 15 TCCAACG 175 0.0 11.939233 18 GGGTCGT 80 2.8767043E-5 11.871395 7 GGCGAGG 660 0.0 11.511906 19 GGTATCA 1385 0.0 11.292999 1 TAGGACT 365 0.0 10.932439 4 TGGCGAG 1635 0.0 10.6298 18 GTAGGAC 1830 0.0 10.591067 3 GTCCTAA 830 0.0 10.456312 1 TGTAGGA 1925 0.0 10.364521 2 GACCGCT 75 0.0026550852 10.130259 7 TTAGGAC 1155 0.0 10.117746 3 GTATCAA 2885 0.0 10.049467 1 GTCCTAC 1675 0.0 10.021048 1 CGGTTTC 95 1.6496565E-4 9.996965 13 CTGTAGG 2000 0.0 9.823189 1 GTCTTAT 195 1.5279511E-10 9.781615 1 >>END_MODULE