FastQCFastQC Report
Thu 26 May 2016
SRR1511764_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511764_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175746
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55310.2542116588976838No Hit
GGTATCAACGCAGAGTACTTTTTTT33560.1542459459881806No Hit
TATCAACGCAGAGTACTTTTTTTTT33240.15277518607410975No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCA500.001551889113.23996459
ACCGTCC550.003174875212.036338
GGTATCA13400.011.4293231
TAGGACC3250.011.0575524
TGCGATG600.00607816711.03330312
GACGTGA3350.010.7274767
TCCAACG3050.010.54236118
GTCAACG750.001910409310.5395572
AGGACCG909.964782E-510.5079085
AACAGTG7700.010.4396757
GTATCAA26700.010.2138961
GGCGAGG4300.010.11691619
ATCGCGC750.00274955410.0875928
TCGCGCG750.00274955410.0875929
GTATAGA3550.010.0200011
GAACAGT6450.09.9702946
GTGCGCC1054.315479E-59.90745611
GTCCTAA5600.09.8808351
GATATAC9500.09.8808351
ACTGTTC11250.09.83540258
Produced by FastQC (version 0.11.5)