##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511764_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2175746 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.581637746317814 31.0 30.0 34.0 25.0 34.0 2 26.902108058569336 31.0 26.0 34.0 10.0 34.0 3 28.581733805324703 31.0 28.0 34.0 16.0 34.0 4 33.113471425432934 35.0 33.0 37.0 28.0 37.0 5 33.56920201163187 35.0 35.0 37.0 28.0 37.0 6 33.8224356151867 37.0 35.0 37.0 30.0 37.0 7 33.87106904942029 37.0 35.0 37.0 30.0 37.0 8 33.89539817607386 37.0 35.0 37.0 30.0 37.0 9 35.51087856762692 39.0 35.0 39.0 30.0 39.0 10 35.39361028355332 39.0 35.0 39.0 30.0 39.0 11 35.40964754157884 39.0 35.0 39.0 30.0 39.0 12 35.321854205408165 39.0 35.0 39.0 30.0 39.0 13 35.3154573190069 39.0 35.0 39.0 30.0 39.0 14 36.47657952720584 40.0 37.0 41.0 30.0 41.0 15 36.41161973870112 40.0 36.0 41.0 29.0 41.0 16 36.34885276130578 40.0 36.0 41.0 29.0 41.0 17 36.32282077043919 40.0 36.0 41.0 29.0 41.0 18 36.258818814328514 40.0 36.0 41.0 29.0 41.0 19 36.299060644027385 40.0 37.0 41.0 29.0 41.0 20 36.22322274750821 40.0 37.0 41.0 29.0 41.0 21 36.14485422471189 40.0 36.0 41.0 28.0 41.0 22 36.0436080314522 40.0 36.0 41.0 28.0 41.0 23 35.942618761565 40.0 36.0 41.0 27.0 41.0 24 35.82409435660229 40.0 36.0 41.0 27.0 41.0 25 35.7173659057629 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9429606091361897 1101 2 3.537314301598464 1101 3 2.1240650220346495 1101 4 1.0058436329381308 1101 5 0.5972400679321197 1101 6 0.5608180183063283 1101 7 0.2778603393930723 1101 8 0.12184899286715023 1101 9 0.1792658069498856 1101 10 0.3668968434581359 1101 11 0.18091082662112257 1101 12 0.20761758456717416 1101 13 0.15560901069044064 1101 14 0.42534134140471735 1101 15 0.20049652927473716 1101 16 0.2960267637798353 1101 17 0.3920655524265442 1101 18 0.4105149639621146 1101 19 0.4070902192236545 1101 20 0.4131648930171963 1101 21 0.41191996360717553 1101 22 0.2982964824301675 1101 23 0.38319969838481427 1101 24 0.33734974281260577 1101 25 0.3724003719015698 1102 1 1.4747491208855372 1102 2 4.136663967737231 1102 3 2.713377182288042 1102 4 1.6661652418924575 1102 5 1.2836566240250846 1102 6 0.9702913308090046 1102 7 0.8086207832573038 1102 8 0.7806853473592952 1102 9 0.9725974103398585 1102 10 0.9789677461675481 1102 11 0.8634775453644323 1102 12 0.8964158446365147 1102 13 0.8743132652810317 1102 14 1.0191258510995311 1102 15 1.1722823123505393 1102 16 1.134346168393968 1102 17 1.1428148675829917 1102 18 1.0684447032935367 1102 19 1.1366596773411928 1102 20 1.0302025166100535 1102 21 1.1308323464347225 1102 22 0.9664020339432469 1102 23 0.8840124150735988 1102 24 0.7808988375350268 1102 25 0.6489286966030221 1103 1 1.2117123110695864 1103 2 3.955226650026276 1103 3 2.5785754792473767 1103 4 1.4824210248553484 1103 5 1.0445855857737172 1103 6 0.8121440580207775 1103 7 0.7488687382675252 1103 8 0.6166534907767769 1103 9 0.7102572799298557 1103 10 0.6422002548000876 1103 11 0.6700803899116892 1103 12 0.7593627670945224 1103 13 0.7516453079241003 1103 14 0.9444061990830406 1103 15 0.7364491047337864 1103 16 0.7891923332000346 1103 17 0.8329538682199527 1103 18 0.9398554948051512 1103 19 0.893340466170855 1103 20 0.8051363058755356 1103 21 0.8014213705387832 1103 22 0.9089998414460112 1103 23 0.7415985728395285 1103 24 0.9405819479309514 1103 25 0.7656617835318471 1104 1 1.4175709030637549 1104 2 4.018766102130996 1104 3 2.4299978607493316 1104 4 1.6038569721310552 1104 5 1.1378199092060655 1104 6 0.9466709769703385 1104 7 0.8369004862275986 1104 8 0.946335201279112 1104 9 0.9150063131153843 1104 10 0.8754070417364517 1104 11 0.945238826000697 1104 12 0.9710252830393671 1104 13 0.851068256353912 1104 14 1.1124041338458426 1104 15 1.0602398273643345 1104 16 0.7853088773697863 1104 17 0.9485089513356257 1104 18 0.8521831386124745 1104 19 0.9887139298975924 1104 20 1.0644096258455704 1104 21 1.054310677874426 1104 22 1.0600962394293703 1104 23 1.1505742556838854 1104 24 1.056038831042578 1104 25 1.008140878067067 1105 1 1.3492610779377578 1105 2 3.7156701702830546 1105 3 2.3949051567497968 1105 4 1.3423294943466715 1105 5 1.0045660640558083 1105 6 0.8624828436621357 1105 7 0.7741069960440257 1105 8 0.6454861998343162 1105 9 0.7267070219856819 1105 10 0.44912762023646735 1105 11 0.4900479335585999 1105 12 0.5537664414890315 1105 13 0.6064951972910535 1105 14 0.49585730346733925 1105 15 0.72261099127428 1105 16 0.5548284208321874 1105 17 0.7432849589510298 1105 18 0.6722163351971417 1105 19 0.647220856724978 1105 20 0.742347185340904 1105 21 0.8081379493277794 1105 22 0.8752560130934128 1105 23 0.8828578134686538 1105 24 0.820185794787271 1105 25 0.9844584732233628 1106 1 0.686197719016377 1106 2 3.304139961122175 1106 3 1.8297039336106025 1106 4 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-1.5843879622226495 2315 9 -1.5925014361397842 2315 10 -1.8067185112443767 2315 11 -1.6480810151019583 2315 12 -1.7779342101743936 2315 13 -1.7223999491292474 2315 14 -1.9306380883095073 2315 15 -2.0029677623915987 2315 16 -2.0949673873387553 2315 17 -1.8655360093914481 2315 18 -2.0328028675869305 2315 19 -2.0835166518231887 2315 20 -2.0811259003514238 2315 21 -2.201134866680029 2315 22 -2.03526097866326 2315 23 -2.117678625835268 2315 24 -2.0660510603173776 2315 25 -2.094959576355272 2316 1 -0.2529226348396527 2316 2 2.1550369811272923 2316 3 0.7018329450211169 2316 4 -0.2603294083390537 2316 5 -0.531623065625773 2316 6 -0.7309006351527003 2316 7 -1.080739903765597 2316 8 -1.0919612237832368 2316 9 -1.1865355204572907 2316 10 -1.1671294256507423 2316 11 -1.0500978832427492 2316 12 -1.3801726611825345 2316 13 -1.326348416611097 2316 14 -1.5309977254240579 2316 15 -1.6070264412472568 2316 16 -1.7023677910604178 2316 17 -1.6047863400255693 2316 18 -1.6700751940910266 2316 19 -1.7277322948775549 2316 20 -1.459617280661874 2316 21 -1.9526615842372834 2316 22 -1.7223540666699435 2316 23 -1.8391808648663357 2316 24 -1.7027624689347505 2316 25 -1.7174467570323202 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 64715.0 3 2.0 4 560.0 5 2830.0 6 3395.0 7 3102.0 8 2976.0 9 2881.0 10 2852.0 11 2727.0 12 2747.0 13 2721.0 14 2598.0 15 2787.0 16 2993.0 17 3155.0 18 3676.0 19 4439.0 20 5786.0 21 7439.0 22 9390.0 23 11034.0 24 13185.0 25 14761.0 26 16706.0 27 19820.0 28 23130.0 29 28234.0 30 35803.0 31 46504.0 32 61008.0 33 82848.0 34 114378.0 35 164747.0 36 263003.0 37 338094.0 38 808720.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.14667245165566 18.63884846852528 13.307573586255014 24.906905493564047 2 16.509462502941275 21.192981276681568 39.56488333341337 22.732672886963787 3 18.823566721483115 24.40045850940321 29.744970230900115 27.03100453821356 4 12.30226322374027 17.107327785504374 38.47558492581395 32.1148240649414 5 13.504839259729767 37.351602622732614 35.69502138576838 13.448536731769241 6 31.35742867044223 37.20797372487413 18.395161935262664 13.039435669420971 7 27.69261669330887 31.65585504925667 22.256917857139573 18.394610400294887 8 24.883695063670118 36.53965122767088 21.012563047341004 17.564090661318 9 26.15282298577132 15.653573532939966 20.34249402273979 37.85110945854893 10 14.807840621101912 29.303282644205712 34.274405192517875 21.614471542174503 11 34.27284251010917 22.975246191421242 24.074501343447256 18.677409955022323 12 22.54877177758801 24.854096020399442 31.919580686348496 20.677551515664053 13 28.535683852802673 21.84106049143604 26.085627642197206 23.53762801356408 14 21.233314918193578 21.814494890488138 28.023445751480182 28.928744439838106 15 23.356494737896796 29.522977406370043 24.119083753342533 23.00144410239063 16 22.51214066347818 28.271728409474267 27.050124417096484 22.16600650995107 17 21.82924845087616 27.842909972027986 27.75521591215151 22.572625664944347 18 21.552423858299637 27.866993665620893 29.032387052532787 21.54819542354668 19 23.06469737951828 27.09044488209786 27.745990453844545 22.09886728453932 20 22.984254595894924 27.347125997244166 28.247231064655526 21.421388342205386 21 23.373278345170682 26.805534298429823 27.443339993133375 22.377847363266124 22 22.7499317471713 27.32480165314326 27.845591418803956 22.079675180881488 23 23.101639769218004 26.901416671990543 27.426159367900382 22.570784190891068 24 22.590744779374422 27.011115732771994 28.108716784365818 22.289422703487773 25 23.08831085981544 26.8790106933438 27.820113193359887 22.212565253480875 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 234.0 1 234.0 2 394.0 3 554.0 4 554.0 5 554.0 6 1400.5 7 2247.0 8 2247.0 9 2247.0 10 3348.5 11 4450.0 12 4450.0 13 4450.0 14 7307.5 15 10165.0 16 10165.0 17 10165.0 18 18073.5 19 25982.0 20 25982.0 21 25982.0 22 40652.5 23 55323.0 24 55323.0 25 55323.0 26 78598.5 27 101874.0 28 101874.0 29 101874.0 30 131964.5 31 162055.0 32 162055.0 33 162055.0 34 181279.0 35 200503.0 36 200503.0 37 200503.0 38 218194.5 39 235886.0 40 235886.0 41 235886.0 42 251911.5 43 267937.0 44 267937.0 45 267937.0 46 275003.0 47 282069.0 48 282069.0 49 282069.0 50 275180.0 51 268291.0 52 268291.0 53 268291.0 54 241986.0 55 215681.0 56 215681.0 57 215681.0 58 185617.5 59 155554.0 60 155554.0 61 155554.0 62 126649.0 63 97744.0 64 97744.0 65 97744.0 66 75120.0 67 52496.0 68 52496.0 69 52496.0 70 38050.0 71 23604.0 72 23604.0 73 23604.0 74 16276.0 75 8948.0 76 8948.0 77 8948.0 78 5931.0 79 2914.0 80 2914.0 81 2914.0 82 1892.5 83 871.0 84 871.0 85 871.0 86 561.5 87 252.0 88 252.0 89 252.0 90 166.5 91 81.0 92 81.0 93 81.0 94 51.0 95 21.0 96 21.0 97 21.0 98 15.5 99 10.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.288919754419863 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 4.596124731471413E-5 20 0.0 21 2.2980623657357064E-4 22 5.515349677765695E-4 23 5.974962150912836E-4 24 4.596124731471413E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2175746.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.33071987383188 #Duplication Level Percentage of deduplicated Percentage of total 1 80.97791178788604 57.76212741709569 2 12.88072767008851 18.375831544123976 3 3.4490761098700307 7.380752454499949 4 1.2343291983695102 3.5218236112394794 5 0.550544178137118 1.9635356274433882 6 0.270476462290066 1.157596845844665 7 0.16108230815065946 0.8043081899527469 8 0.09811817596972067 0.559907209970199 9 0.07487002189278884 0.4806479302723963 >10 0.270097760940566 3.366862257890107 >50 0.01829573181348973 0.902783215710166 >100 0.012256167576114729 1.7939648132073591 >500 0.0012374739203877092 0.6097127582382144 >1k 9.11822888706258E-4 1.063187783623593 >5k 6.513020633616129E-5 0.2569583408880093 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5531 0.2542116588976838 No Hit GGTATCAACGCAGAGTACTTTTTTT 3356 0.1542459459881806 No Hit TATCAACGCAGAGTACTTTTTTTTT 3324 0.15277518607410975 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.596124731471413E-5 0.0 5 0.0 0.0 0.0 4.596124731471413E-5 0.0 6 0.0 0.0 0.0 4.596124731471413E-5 0.0 7 0.0 0.0 0.0 4.596124731471413E-5 0.0 8 0.0 0.0 0.0 4.596124731471413E-5 0.0 9 0.0 0.0 0.0 4.596124731471413E-5 0.0 10 0.0 0.0 0.0 4.596124731471413E-5 0.0 11 0.0 0.0 0.0 4.596124731471413E-5 0.0 12 0.0 0.0 0.0 4.596124731471413E-5 0.0 13 0.0 4.596124731471413E-5 0.0 4.596124731471413E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTCCA 50 0.0015518891 13.2399645 9 ACCGTCC 55 0.0031748752 12.03633 8 GGTATCA 1340 0.0 11.429323 1 TAGGACC 325 0.0 11.057552 4 TGCGATG 60 0.006078167 11.033303 12 GACGTGA 335 0.0 10.727476 7 TCCAACG 305 0.0 10.542361 18 GTCAACG 75 0.0019104093 10.539557 2 AGGACCG 90 9.964782E-5 10.507908 5 AACAGTG 770 0.0 10.439675 7 GTATCAA 2670 0.0 10.213896 1 GGCGAGG 430 0.0 10.116916 19 ATCGCGC 75 0.002749554 10.087592 8 TCGCGCG 75 0.002749554 10.087592 9 GTATAGA 355 0.0 10.020001 1 GAACAGT 645 0.0 9.970294 6 GTGCGCC 105 4.315479E-5 9.907456 11 GTCCTAA 560 0.0 9.880835 1 GATATAC 950 0.0 9.880835 1 ACTGTTC 1125 0.0 9.8354025 8 >>END_MODULE