FastQCFastQC Report
Thu 26 May 2016
SRR1511764_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511764_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175746
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT70280.32301564612781086No Hit
TATCAACGCAGAGTACTTTTTTTTT46950.2157880561425828No Hit
GGTATCAACGCAGAGTACTTTTTTT43230.19869047214150914No Hit
TTGTAGAACAGTGTATATCAATGAG21980.10102282159774165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG3150.012.05952318
TCGGAAC658.015347E-411.6941043
CCAACGA2050.011.58155419
GCGACTA1104.992271E-711.22357816
CGACTAA1104.992271E-711.22357817
AGCATCG600.005870219311.0850375
TAACCGC600.00589134911.0796866
GGTATCA15600.011.0666231
CGGTTTC2500.011.01637613
TAGGACC2950.010.9508354
GTGTTCG909.54997E-510.5520839
TATACTG3700.010.5286455
GTCCTAA5800.010.5247811
GTATTAG2001.8189894E-1210.4918921
GTACTAA2001.8189894E-1210.4918921
CGCCCTA1752.3646862E-1010.31089316
GCCGGTT2600.010.22740311
TAAGCGA1303.860332E-710.22740313
CCTATAC1409.670839E-810.1801363
GTCCTAC10050.010.1549751
Produced by FastQC (version 0.11.5)